Publications by authors named "Xiang Man Zhang"
[Detection of microvasculature in rat hind limb using synchrotron radiation].
Zhonghua Yi Xue Za Zhi
March 2012
Article Synopsis
- The study aimed to use synchrotron radiation and microangiographic techniques to identify deep-level microvascular structures in rat hind limbs.
- It involved innovative imaging methods, including in vivo and ex vivo microangiography, using specific contrast media to visualize blood vessels in high detail.
- The findings revealed that small blood vessels down to 9 µm could be effectively visualized, highlighting the potential of these techniques for analyzing microvasculature and angiogenesis in lower limbs.
Article Synopsis
- The study aimed to assess the impact of superparamagnetic iron oxide (SPIO) labeling on murine embryonic stem (ES) cell viability, proliferation, and differentiation into endothelial cells.
- Labeling was accomplished using CLIO-Tat, which showed over 96% cell incorporation without negatively affecting ES cell viability.
- The resulting endothelial cells from the labeled ES cells closely resembled normal aortic endothelial cells in terms of their structure and marker expression.
Background: Varicose veins (VVs), a common vascular disease, are functionally characterized by dilation and tortuosity and are widely prevalent in the adult population. The pathophysiology and molecular mechanism of VVs are still unclear. A genetic risk for VVs has been demonstrated, although no genetic variant pertaining to VVs has been identified.
View Article and Find Full Text PDFDeveloping cost-effective methods for high throughput production of recombinant baculoviruses in insect cells is very challenging, because the baculovirus DNA preparation and the following transfection procedure are labour-intensive and time consuming. We developed a new method of introducing recombinant Bacmid DNA from bacteria into insect cells simply using invasive diaminopimelate (DAP) auxotrophic Escherichia coli to infectSpodoptera frugiperda 9 cells. The E.
View Article and Find Full Text PDF[Screening for peptides of anti-rotavirus by phage-displayed technique].
Sheng Wu Gong Cheng Xue Bao
May 2007
In this study, a 15-mer phage display peptide library was employed to pan against human rotavirus immobilized on solid phase. 4 different peptides were selected and could bind with rotavirus particles specifically. Plaque reduction neutralization test and MTT analysis results indicated that 3 of the peptides can inhibit rotavirus infecting in vitro.
View Article and Find Full Text PDFArticle Synopsis
- In the post-genomic era, efficient and cost-effective systems for eukaryotic protein expression, like the baculovirus system, are increasingly important.
- A new method using MAGIC allows for the quick and high-efficiency creation of recombinant BmBacmid vectors in silkworms, achieving a 99.8% success rate in producing positive recombinants.
- This research enabled the high-level expression of various genes in silkworm larvae and proposed the potential to create a high-quality baculovirus cDNA library through plasmid transfer.