Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis RPL11-MDM2-p53 Pathway in Mouse Spermatogonia.

Objective: The expression patterns of ribosomal large subunit protein 23a (RPL23a) in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23a expression and spermatogonia apoptosis upon exposure to X-ray.

Methods: Male mice and GC-1 cells were irradiated with X-ray, terminal dUTP nick end-labelling (TUNEL) was performed to detect apoptotic spermatogonia . Apoptotic rate and cell cycle phase of GC-1 cells were analyzed with flow cytometry.