Publications by authors named "Xianfei Gao"
Assay for interferon gamma release as a novel marker in pediatric patients with systemic lupus erythematosus.
Pediatr Rheumatol Online J
August 2024
Background: The interferon-gamma (IFN-γ) release assay (IGRA) is an important laboratory diagnosis for latent Mycobacterium tuberculosis (TB) infection. The TB-IGRA measures the release of IFN-γ from peripheral blood cells, who are exposed to TB antigen (Ag), mitogen (MT), or negative/nil control (NL) in vitro. While, an exceptional higher TB Ag-NL level will reflect an elevation of peripheral lymphocytes released IFN-γ in a same condition.
View Article and Find Full Text PDFMulticentric carpotarsal osteolysis syndrome with variants of MAFB gene: a case report and literature review.
Pediatr Rheumatol Online J
March 2024
Article Synopsis
- MCTO is a rare genetic disorder leading to bone loss in the hands, feet, and other areas, often mimicking juvenile idiopathic arthritis, which complicates diagnosis.
- Identification of MAFB mutations is a key advancement in understanding MCTO's causes.
- Early MRI and whole exome sequencing proved effective for accurate diagnosis, and Denosumab treatment showed positive outcomes in a patient case.
Background: The range of pulmonary complications beyond infections in pediatric immunocompromised patients is broad but not well characterized. Our goal was to assess the spectrum of disorders with a focus on interstitial lung diseases (ILD) in immunodeficient patients.
Methods: We reviewed 217 immunocompromised children attending a specialized pneumology service during a period of 23 years.
Article Synopsis
- The study focuses on a specific genetic deficiency related to ZNFX1, which is crucial for regulating antiviral immune responses to viral nucleic acids.
- Researchers examined 15 patients from 8 families who showed severe reactions to both RNA and DNA viral infections, including chronic inflammation and organ issues.
- The findings revealed harmful genetic variants in ZNFX1 among patients, leading to unbalanced interferon-stimulated gene expression and difficulties in clearing viral infections.
[The effect of Bcl-2 gene silencing on the sensitivity of cell line A549 to chemotherapeutic drugs].
Zhonghua Jie He He Hu Xi Za Zhi
March 2013
Objective: To investigate the effects of miRNA-mediated down-regulation of the Bcl-2 gene on the chemotherapeutic sensitivities and mRNA transcriptions of sensitivity associated genes in human lung adenocarcinoma cell line A549 cells, and therefore to provide experimental data for improving the chemotherapeutic effects on non-small cell lung cancer (NSCLC).
Methods: The miRNA recombinant plasmid targeting to human Bcl-2 gene was designed, synthesized and stably transferred into A549 cells by lipofectin technique as the experiment group. The transcription of Bcl-2 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) by agarose gel electrophoresis, real-time PCR, and the protein level of Bcl-2 was measured by Western blot to confirm the function of miRNA plasmid.