Substance P augments PGE2 and IL-6 production in titanium particles-stimulated fibroblasts from hip periprosthetic membrane.

Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Implant-derived particles are thought to be a main cause of osteolysis that leads to failure of total joint arthroplasty. The nervous system has been implicated in the etiology and pathogenesis of joint diseases.

Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development.

Sea-island cotton (Gossypium barbadense L.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. We constructed a normalized fiber cDNA library (from -2 to 25 dpa) of G.

[Advance in quantitative traits from phenotypic variation to gene discovery].

With the rapid development of molecular biotechnology, many improved approaches have been provided for studying on the genetic bases of quantitative traits. Many studies paid attention to dissect the genetic bases using different emendatory approaches from phenotypic variation to gene discovery, mainly containing QTL mapping, chemical mutagenesis etc. Especially, QTL analysis was executed for a lot of important quantitative traits.

Relationship between gene differential expression of leaves in full opening flower stages of hybrids & their parents and heterosis in pest-resistant cotton.

cDNAs of leaves of 24 pest-resistant cotton (authorization) crosses and their parents in full opening flower stages were amplified and different bands were displayed by DDRT-PCR, with 45 primer combinations. The mean percentage of bands duplicated was 70.1%, which implied that there was a high proportion of pseudopositive fragments in the amplified cDNA.

[Expression of transforming growth factor beta1 and insulin-like growth factor I in the regenerated bones after low frequency micro movement].

Objective: To investigate the expression of transforming growth factor beta1 (TGF-beta1) and insulin-like growth factor I (IGF-I) in new bone after low frequency micro movement.

Methods: Fifteen female sheep from Shandong province were involved in the study and their bilateral tibias transversely osteotomized in the middle shafts with a defect of 2 mm. The hind limbs were fixed with unilateral external fixators connected to a controlled micro movement device.

[Minimally invasive total hip arthroplasty with anterior incision].

Objective: To report the clinical outcome of minimally invasive total hip arthroplasty with anterior incision.

Methods: One hundred and twenty cases were randomly divided into two groups. Sixty cases (group 1) who had undergone a mini-invasive THA were compared with 60 cases (group 2) who had undergone THA with standard posterolateral incision.

Function analysis of promoter trapping system after inserted into cotton (Gossypium hirsutum L. ) genome.

The technique of promoter trapping was developed to investigate its viability in cotton ( Gossypium hirsutum L.) functional genomics. 141 independent transformants of cotton were generated via Agrobacterium tumefaciens mediated transformation, of which 97% showed positive by PCR detection.

Isolation and characterization of genes associated to cotton somatic embryogenesis by suppression subtractive hybridization and macroarray.

Somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway and is a notable illustration of cell totipotency. To identify genes involved in SE, subtractive polymerase chain reaction (PCR) was performed to generate transcripts highly enriched for SE-related genes, using cDNA prepared from a mixture of embryogenic callus and pre-globular somatic embryos, as the tester, and cDNA from non-embryogenic callus, as the driver. After differential screening and subsequent confirmation by reverse Northern blot analysis, a total of 671 differentially expressed cDNA fragments were identified, and 242 uni-genes significantly up-regulated during cotton SE were recovered, as confirmed by Northern blot and reverse-transcription PCR analysis of representative cases, including most previously published SE-related genes in plants.

[A primary study on simulation of fracture strength based on BP neural networks].

Objective: To model the relationship between stimulating stress and fracture strength using BP neural networks, and to provide a theoretical basis for accurate prediction of the rate of fracture healing.

Methods: The bilateral tibiae in New Zealand rabbits were osteotomized and fixed by stress-relaxation plate(SRP) and rigid plate(RP), respectively. The stress shielding rate and bending strength of the healing fractures were measured at 2 to 48 weeks postoperatively.

Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium) species.

Calli were successfully induced from hypocotyls of eight wild diploid cotton species (Gossypium) on MSB (MS salts and B(5) vitamins) medium supplemented with 0.09 microM 2,4-D (2,4-dichlorophenoxyacetic acid) and 2.32 microM KT (kinetin).

[Effect of vascular endothelial cell growth factor on repair of bone defect with cortical bone allograft].

Objective: To study the effect of vascular endothelial cell growth factor (VEGF) on repair of bone defect with cortical bone allograft.

Methods: Forty five New Zealand white rabbits, weighted 2.5-3.

A first order system model of fracture healing.

A first order system model is proposed for simulating the influence of stress stimulation on fracture strength during fracture healing. To validate the model, the diaphyses of bilateral tibiae in 70 New Zealand rabbits were osteotomized and fixed with rigid plates and stress-relaxation plates, respectively. Stress shielding rate and ultimate bending strength of the healing bone were measured at 2 to 48 weeks postoperatively.

[Construction and analysis of SSH library of Gossypium barbadense upon infection with Verticillium dahliae].

Roots were collected from the seedlings inoculated with pathogen Verticillium dahliae after 2, 4, 8, 12, 24, 48, 72 and 96 hours for total RNA extraction. The cDNAs from the inoculated seedlings were used as the tester and those from the control seedlings as the driver. SSH method was employed to find the differently expressed cDNAs responding to the pathogen.

[Withdrawal of exogenous auxin induces programmed cell death of cotton embryogenic suspension cultures].

In the establishment of cotton suspension culture, we had observed an interesting phenomenon that large-scale cell death occurred when the embryogenic cells were transferred from the medium MS supplemented with IBA 0.5 mg/L to fresh MS medium without IBA. Cytological study and genomic DNA electrophoresis showed that this kind of cell death was accompanied by such morphological characters as chromatin condensation, the maintenance of membrane continuity, a condensed cytoplasm and evident DNA fragmentation of multimers of 140-180 bp.

[Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton].

SRAP (Sequence-related Amplified Polymorphism), a new marker system, was applied in cotton genome analysis. We developed an efficient PCR reaction system for detecting SRAP that showed reliable, effective and reproductive. SRAP marker was used to detect the polymorphisms between 'Pima90' (G.

[Transgenic cotton plants of chitinase and glucanase genes and their performance of resistance to Verticillium dahliea].

The two disease-resistance genes chitinase and glucanase, which were respectively directed by commelina yellow mottle virus promoter (CoYMV, vascular specific) and CaMV35S promoter, were introduced into cotton genome via Agrobacterium tumefaciens. Transgenic plants were obtained from two popularly cultivated varieties Jihe321 and CRIC35. After screening by spraying kanamycin over unfolding leaves, the kanamycin resistance (KmR) plants were tested by PCR and Southern blot.

Rescue of defective auxin-mediated gene expression and root meristem function by inhibition of ethylene signalling in sterol biosynthesis mutants of Arabidopsis.

The roles of sterols in plant development are not well understood, but evidence is emerging that they are required for cell division, polarity and patterning by mechanisms that are independent of brassinosteroids, of which they are precursors. Previous evidence shows that two sterol-defective mutants of Arabidopsis thaliana (L.) Heynh.

Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion.

Symmetric somatic hybrid plants between Gossypium hirsutum Coker 201 and G. klotzschianum were obtained through electrofusion. The fusion products were cultured in KM8P medium supplemented with 2.

[Inheritance and segregation of transformants in cotton with two types of insect-resistant genes].

A plant expression vector containing a chemeric Bt29K gene coding for the active Cry1Ac protein and the arrowhead proteinase inhibition gene API-B was introduced into an elite cotton cultivar Jihe 321 by Agrobactertium tumefaciens. Some insect-resistant cotton lines were developed. Segregation and stabilization of insect-resistant genes in six transformation lines were studied.

Localization of QTLs for yield and fiber quality traits of tetraploid cotton cultivar.

Using SSR and RAPD as molecular markers, and the 69 F2 families from a cross between Handan208 (Gossypium hirsutum) and Pima90 (Gossypium barbadense) as a mapping population, a linkage map comprising 126 markers was constructed. With an average spacing 13.7 cM between markers, the linkage map spanned 1,717.

The effects of stress-relaxation plate on disorganization and repair of regional bone structure: an experimental study.

OBJECTIVE: To study the influence of stress-relaxation plate on disorganization and repair of the cortex beneath the plate. METHODS: A washer made of viscoelastic polyethylene was placed between the screw and the screw hole of conventional stainless rigid plate (RP) to produce a stress-relaxation plate (SRP). Both SRP and RP were applied to osteotomized tibia in 48 New Zealand rabbits.

Experimental study of effect of stress-relaxation bone plate on fracture healing.

OBJECTIVE: To study the change of the stress shi elding rate of stress-relaxation plate in vivo and its influence on fracture he aling. METHODS: The diaphyses of bilateral tibias in 70 New Zealand ra bbits were osteotomized and fixed with stress-relaxation plates (SRP, the SRP g roup) and rigid plates (RP, the RP group), respectively. The fracture healing pr ocess in these 2 groups was investigated by radiography, light and polarized l ight microscopy and biomechanical test at 2 to 48 weeks postoperatively.