Corrigendum: Microbiome and response surface methodology analyses reveal as the core bacteria responsible for aerobic spoilage of corn silage () in hot and humid areas.

[This corrects the article DOI: 10.3389/fmicb.2024.

Hazardous risk of antibiotic resistance genes: Host occurrence, distribution, mobility and vertical transmission from different environments to corn silage.

Antibiotic resistance genes (ARGs) are one of the emerging contaminants posing a great deal of hazardous risk to public health. This study employed metagenomics and deciphered the potential risk of the antibiotic resistome and their vertical transfer to ensiled whole-crop corn silage harvested from six climate zones: 1. Warm temperate-fully humid-hot summer (Cfa), 2.

Detoxifying mycotoxins and antifungal properties of two rumen-derived Enterococcus species in artificially contaminated corn silages.

Background: Mycotoxins contamination in food and feed has emerged as an issue of serious concern because they pose serious health risks to both humans and livestock. The study aimed to evaluate the effects of two rumen-derived Enterococcus spp. on fermentation and hygienic quality of artificially contaminated corn silages.

Altitudinal Distribution Patterns of Phyllosphere Microbial Communities and Their Contribution to Silage Fermentation of Along the Elevation Gradient on the Tibetan Plateau.

The study aimed to reveal altitudinal distribution patterns of phyllosphere microbial communities and silage fermentation of along the elevation gradient on the Tibetan Plateau. The was individually collected from 2,500, 3,000, 4,000, 4,500, and 5,000 m above sea level (a.s.

The Effect of Early and Delayed Harvest on Dynamics of Fermentation Profile, Chemical Composition, and Bacterial Community of King Grass Silage.

The objective of this study was to assess the effect of harvesting time on the fermentation characteristics, chemical composition, and microbial community of king grass silage. King grass was harvested at three growth periods of 90 days (KN90S), 110 days (KN110S), and 130 days (KN130S); chopped into 2-3-cm particle size; and ensiled in polyethylene bags (20 × 30 cm). The fermentation quality and chemical composition of silages were analyzed after 1, 3, 7, 14, 30, and 60 days of ensiling.

Mycotoxins in Maize Silage from China in 2019.

Animal feed (including forage and silage) can be contaminated with mycotoxins. Here, 200 maize silage samples from around China were collected in 2019 and analyzed for regulated mycotoxins, masked mycotoxins (deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, and deoxynivalenol-3-glucoside), and emerging mycotoxins (beauvericin, enniatins, moniliformin, and alternariol). Deoxynivalenol and zearalenone were detected in 99.

Effects of hexanoic acid on microbial communities, fermentation, and hygienic quality of corn silages infested with toxigenic fungi.

Background: This study aims to reveal the effects of hexanoic acid on the microbial communities, fermentation, and the hygienic quality of corn silages with or without fungal infection. Fungal-infested (FI) and non-infested (NFI) whole-crop corn samples were separately ensiled without (control, CON) or with hexanoic acid (Hex, 90% purity) at 0.2 g·kg fresh weight (FW).

Effect of Lactic Acid Bacteria on the Fermentation Quality and Mycotoxins Concentrations of Corn Silage Infested with Mycotoxigenic Fungi.

This study was conducted to evaluate the effect of lactic acid bacteria (LAB) on fermentation quality, mycotoxin concentrations, and microbial communities of whole-crop corn silages infested with mycotoxigenic fungi. Cultured spores (10 cfu/mL) of mycotoxigenic and were sprayed (5 mL) on corn forage on 27 July and 10 August 2018. On 21 August 2018, sprayed (FI; 3 plots) and unsprayed (NFI; 3 plots) corn forage were harvested at the 1/2 kernel milk line stage, followed by chopping and ensiling without inoculants (CON), or with (LB, 1 × 10 cfu/g FW), (LT, 1 × 10 cfu/g FW), or + (BT: both and applied at 0.

Effect of hexanoic acid, Lactobacillus plantarum and their combination on the aerobic stability of napier grass silage.

Aims: The objective of this study was to evaluate the potential of hexanoic acid (Hex) as a silage additive.

Methods And Results: The effect of Hex, Lactobacillus plantarum (Lp) and their combination (Hex + Lp) on the aerobic stability of napier grass silage was investigated. Napier grass was ensiled without additives (C) or with Lp, Hex or Hex + Lp for 60 days followed by 7 days of aerobic exposure.

Effect of alfalfa microbiota on fermentation quality and bacterial community succession in fresh or sterile Napier grass silages.

The aim of this study was to reveal the reconstitution dynamics of alfalfa microbiota and their contribution to the fermentation quality of Napier grass silages. Napier grass was harvested at approximately 20% dry matter content, chopped to a theoretical length of cut of 2 to 3 cm, and ensiled in laboratory polyethylene plastic bags (400 × 250 mm) for 30 d. The Napier grass was treated with the following: natural fermentation and distilled water (NG), inoculum of alfalfa microbiota (AM), gamma-ray irradiation and distilled water (IR), and gamma-ray radiation and alfalfa microbiota (IR+AM).

The reconstitution mechanism of napier grass microiota during the ensiling of alfalfa and their contributions to fermentation quality of silage.

To reveal the reconstitution mechanism of exogenous microbiota and their contributions to fermentation quality during the early stage of alfalfa ensiling. The chopped alfalfa was treated with the following: distilled water (A1); napier grass microbiota (A1N); γ-ray radiation + distilled water (A0); γ-ray radiation + napier grass microbiota (A0N). Inoculating napier grass microbiota to non-irradiated alfalfa decreased the LA concentration, while enhanced the LA production of irradiated alfalfa during the 7 d of ensiling.

Comparison of nitrogen transformation dynamics in non-irradiated and irradiated alfalfa and red clover during ensiling.

Objective: To study the contribution of plant enzyme and microbial activities on protein degradation in silage, this study evaluated the nitrogen transformation dynamics during ensiling of non- and irradiated alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.).

Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant.

To reveal the mechanism of the survival and adaption of inoculated Lactobacillus plantarum during ensiling. Alfalfa was ensiled directly (A1), after γ-ray irradiation (A0), and after inoculation of the sterile (A0L) or fresh alfalfa (A1L) with Lactobacillus plantarum. The A0L had the higher lactic acid content and lower pH than that in A1L from 3 days of ensiling.

The effects of fibrolytic enzymes, cellulolytic fungi and bacteria on the fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields of Pennisetum sinese silage.

Biological inoculants were tested on Pennisetum sinese for their effects on fermentation characteristics, structural carbohydrates degradation, and enzymatic conversion yields. Pennisetum sinese was ensiled without additive, Lactobacillus plantarum (Lp), Trichoderma reesei (Tr), fibrolytic enzymes (E), and Enterococcus faecium (Y83) for 90 days. Y83 silages had higher LA and lower AA, ammonia-N and DM loss as compared to E and Tr silages.

Characterization of Enterococcus faecalis JF85 and Enterococcus faecium Y83 isolated from Tibetan yak (Bos grunniens) for ensiling Pennisetum sinese.

Two bacteria strains with cellulolytic potential isolated from Tibetan yak (Bos grunniens) rumen were identified as Enterococcus faecalis (JF85) and Enterococcus faecium (Y83). Isolates grow well within a range of temperature 15 to 55 °C and pH 3.0-7.

Effects of applying oil-extracted microalgae on the fermentation quality, feed-nutritive value and aerobic stability of ensiled sweet sorghum.

Background: A laboratory-silo study was conducted to evaluate the fermentation quality, feed-nutritive value and aerobic stability of sweet sorghum silage with or without oil-extracted microalgae supplementation. Sweet sorghum was mixed with four microalgae levels (0%, 1%, 2% and 3% on a dry matter basis; control, M1, M2 and M3, respectively) and ensiled for 45 d. Further, the four experimental silages were subjected to an aerobic stability test lasting 7 d.

Isolation and molecular identification of lactic acid bacteria from King grass and their application to improve the fermentation quality of sweet Sorghum.

The aim of the present study was isolation and molecular identification of lactic acid bacteria from King grass and their application to improve the fermentation quality of sweet Sorghum. Seventy-six strains of LAB were isolated; five strains were selected for Physiological and morphological tests and 16S rRNA sequencing. All five strains grew at different pH 3.

Microbiological and chemical profiles of elephant grass inoculated with and without Lactobacillus plantarum and Pediococcus acidilactici.

The study was conducted to evaluate the microbiological and chemical profiles of elephant grass inoculated with and without different wild strains of lactic acid bacteria. Silage was prepared of four treatments and one control with three replicates as control (EKC, adding 2 ml/kg sterilizing water), Lactobacillus plantarum (USA commercial bacteria) (EKP), Lactobacillus plantarum (EKA), Pediococcus acidilactici (EKB), and Pediococcus acidilactici (SKD) isolated from King grass. Silage were prepared using polyethylene terephthalate bottles, and incubated at room temperature for different ensiling days.

Isolating and evaluating lactic acid bacteria strains for effectiveness on silage quality at low temperatures on the Tibetan Plateau.

Four lactic acid bacteria (LAB) strains isolated from straw silages on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) at different temperatures (10°C, 15°C and 25°C) were studied. These LAB isolates were evaluated using the acids production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests.

Effects of calcium propionate on the fermentation quality and aerobic stability of alfalfa silage.

Objective: To assess the potency of calcium propionate (CAP) used as silage additive, an experiment was carried out to evaluate the effect of CAP on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages.

Methods: Alfalfa was ensiled with four levels of CAP (5, 10, 15, and 20 g/kg of fresh weight [FW]) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test.

Effects of sodium diacetate on the fermentation profile, chemical composition and aerobic stability of alfalfa silage.

Objective: The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa ( L.) silage.

Methods: Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7, and 9 g/kg of fresh forage).

Cloning, expression and characterization of a cold-adapted endo-1, 4--glucanase from A1, a symbiotic bacterium of .

Background: Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study, A1 which was isolated from a wood-inhabiting termite could secrete a cold-adapted EglC. However, its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.

Characteristics of isolated lactic acid bacteria and their effects on the silage quality.

Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass ( Lam.) silage were studied.

Methods: The four isolated strains and one commercial inoculant (G, MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests.