Full utilization of Flammulina filiformis spent mushroom substrate for sustainable production of oil/water separation bacterial nanocellulose composite with high liquid flux and seperation efficiency.

Bacterial nanocellulose (BNC) is a promising material for oily wastewater separation due to its hydrophilic and nanometer-scale porous structure. However, its application is constrained by high production costs. This study explores the utilization of underutilized Flammulina filiformis spent mushroom substrate (Ff-SMS) to produce BNC cost-effectively.

Phylogenetic and Morphological Perspectives on subg. : Exploratively Unveiling Hidden Diversity in China.

subg. contributes to more than half of species and exhibits highly hidden diversity. However, .

Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection in .

Oyster mushroom spherical virus (OMSV) is a mycovirus that inhibits mycelial growth, induces malformation symptoms, and decreases the yield of fruiting bodies in . However, the pathogenic mechanism of OMSV infection in is poorly understood. In this study, RNA sequencing (RNA-seq) was conducted, identifying 354 differentially expressed genes (DEGs) in the mycelium of during OMSV infection.

Effects of mating-type ratio imbalance on the degeneration of subculture and preventative measures.

The rapid degeneration of strains during subculture represents a bottleneck problem that affects production stability. This study explored the mechanism underlying this degeneration in three production and three wild-type strains of , isolating single-conidium strains from each. The effects of subculturing on fructification in both original and single mating-type strains were compared.

Revealing Brownish Diversity in China: New Discoveries and Taxonomic Insights.

Within the genus , species exhibiting brownish basidiomata present considerable challenges in identification due to similar coloration. This study underscores the significance of pileipellis types and cheilocystidia characteristics as critical in delimiting brownish species. To clarify the principal taxonomic characters and their utility in distinguishing between brownish species, a morphological taxonomy and phylogenetic analysis were performed.

Transmission of Oyster Mushroom Spherical Virus to Progeny via Basidiospores and Horizontally to a New Host .

Mycoviruses are usually transmitted horizontally via hyphal anastomosis and vertically through sporulation in natural settings. Oyster mushroom spherical virus (OMSV) is a mycovirus that infects , with horizontal transmission via hyphal anastomosis. However, whether OMSV can be vertically transmitted is unclear.

Effect of addition of γ-poly glutamic acid on bacterial nanocellulose production under agitated culture conditions.

Background: Bacterial nanocellulose (BNC), a natural polymer material, gained significant popularity among researchers and industry. It has great potential in areas, such as textile manufacturing, fiber-based paper, and packaging products, food industry, biomedical materials, and advanced functional bionanocomposites. The main current fermentation methods for BNC involved static culture, as the agitated culture methods had lower raw material conversion rates and resulted in non-uniform product formation.

Morphological and phylogenetic analyses reveal five new species of Porotheleaceae (Agaricales, Basidiomycota) from China.

The first occurrence of and in the Chinese mycobiota are reported, and , two new species and , a new combination, revealed by phylogenetic analyses and morphological study. These newly-recorded genera, , which can be distinguished by their agaricoid basidiomata, dark-coloured stipe, sarcodimitic tramal structure, stipitipellis with yellow to yellowish-brown pigments and yellow-pigmented thick-walled caulocystidia and , which differs from other genera of Porotheleaceae by its pruinose stipe, decurrent lamellae, inamyloid basidiospores and absence of hymenial cystidia. We also formally describe three other new species of Porotheleaceae collected from Chinese temperate to subtropical zones of Fujian and Zhejiang Provinces: , and .

Biological characteristics of Cordyceps militaris single mating-type strains.

Cordyceps militaris has been extensively cultivated as a model cordyceps species for commercial purposes. Nevertheless, the problems related to strain degeneration and breeding technologies remain unresolved. This study assessed the physiology and fertility traits of six C.

Comparative Analysis of Agronomic Traits, Yield, and Effective Components of Main Cultivated Ganoderma Mushrooms (Agaricomycetes) in China.

To assess the strain resources and address production challenges in Ganoderma cultivation. 150 Ganoderma strains were collected from 13 provinces in China. A comparative analysis of agronomic traits and effective components was conducted.

Population genetics provides insights into the important agronomic traits of Ganoderma cultivation varieties in China.

This study aimed to investigate the species diversity and genetic differentiation of the genome of the main cultivated strains of Ganoderma in China. Population genomics analysis was conducted based on 150 cultivated strains of Ganoderma collected nationwide. The results indicated that the main species currently cultivated in China were Ganoderma sichuanense and Ganoderma lucidum, with a minor proportion of Ganoderma sessile, Ganoderma weberianum, Ganoderma sinense, Ganoderma gibbosum and Ganoderma australe.

Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host .

Oyster mushroom spherical virus (OMSV) is a mycovirus with a positive-sense single-stranded RNA genome that infects the edible mushroom . OMSV is horizontally transferred from an infected strain to a cured strain via mycelia. The infection results in significant inhibition of mycelial growth, malformation of fruiting bodies, and yield loss in oyster mushrooms.

Transcriptome and metabolome profiling unveils the mechanisms of naphthalene acetic acid in promoting cordycepin synthesis in .

Cordycepin, an important active substance in , possesses antiviral and other beneficial activities. In addition, it has been reported to effectively promote the comprehensive treatment of COVID-19 and thus has become a research hotspot. The addition of naphthalene acetic acid (NAA) is known to significantly improve the yield of cordycepin; however, its related molecular mechanism remains unclear.

Four new species of Mycenasect.Calodontes (Agaricales, Mycenaceae) from northeast China.

Species of Mycenasect.Calodontes are representative of the genus as a whole and are easily recognised by the pinkish, reddish, purplish to brownish pileus and larger basidiomata. Furthermore, the colour of the pileus in the species of sect.

Updated taxonomy on (Porotheleaceae, Agaricales) with three new taxa and one new record from China.

Only three (Porotheleaceae) species have been previously recorded in China. Here, we report collections of a fourth species in China: Har. Takah.

Taxonomic studies of bluish (Mycenaceae, Agaricales) with two new species from northern China.

Bluish are rare, but constitute a taxonomically complex group. A total of eight bluish species in four sections have previously been reported from North America, Europe, Oceania and Asia. Two species with a blue pileus, collected in China during our taxonomic study of s.

Multiplex Detection of Mycoviruses.

Mycoviruses are viruses that specifically infect and replicate in fungi. Several mycoviruses have been previously reported in , including the oyster mushroom spherical virus (OMSV), oyster mushroom isometric virus (OMIV), spherical virus (POSV), and virus 1 (PoV1). This study was designed to develop a multiplex RT-PCR for simultaneous detection and differentiation of the four mycoviruses.

Study on Characteristics of Triterpenoids and Hepatoprotective Effects of Fruit Body of Stout Camphor Mushroom, Taiwanofungus camphoratus (Agaricomycetes), Cultivated with Apple-Wood.

Taiwanofungus camphoratus is a parasite medicinal fungus with significant hepatoprotective activity that grows in Cinnamomum camphora, a class II protected tree species in Taiwan. Currently, commercial cultivation of T. camphoratus is limited by the resources of C.

Preliminary Studies on the Effects of Oyster Mushroom Spherical Virus China Strain on the Mycelial Growth and Fruiting Body Yield of the Edible Mushroom .

Oyster mushroom spherical virus (OMSV) is a positive-sense single-stranded RNA mycovirus which is associated with a devastating oyster mushroom die-back disease. However, little is known about its diversity, and the effects of OMSV infection on its fungal host are not well understood. In this study, we determined the nearly complete nucleotide sequence of OMSV isolated from cultivated oyster mushrooms in China.

Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea.

Background: Antrodia cinnamomea, a rare medicinal fungus, has been increasingly studied in recent years because of its abundant secondary metabolites which are beneficial to humans. However, there is a lack of research on its polyphenols which are of good research value due to their antioxidant, anti-inflammatory, hypoglycemic and other activities.

Results: In this study, the effects of different extraction conditions on the yield of its polyphenols were investigated.

sp. nov. and found in subalpine areas of China.

Only a few species have been previously reported from subalpine areas of China. Members of possessing an orange-yellow pileus are rare in China as well. Here, we describe , a new species characterized by an orange-yellow pileus, ovoid basidiospores, and abundant cylindric to narrowly utriform cheilocystidia.

New Species of (Fungi) Isolated from Soil in Southwestern China.

Southwestern China belongs among the global biodiversity hotspots and the Daba Mountains are recognized as one of the priority conservation areas. During the exploration of fungal biodiversity from soil samples collected from Mount Daba, two species of were discovered as new to science based on phylogenetic analyses and morphological comparisons. sp.

Updated description of (Mycenaceae, Agaricales), including three new species with brightly coloured pilei from Yunnan Province, southwest China.

An updated description of the genus , combining macro- and micromorphological characters that elaborate on the original generic characterisation, is presented. is characterised by a brightly coloured pileus, all tissues inamyloid and pileipellis covered with simple to branched excrescences. Previously, nine species were known globally, of which three species were accepted in China.

Optimization of Extraction Process and the Antioxidant Activity of Phenolics from .

, is a widely used medicinal fungus. The polyphenols extracted from this fungus exert antioxidant, anti-inflammatory, and hypoglycemic effects. In this study, polyphenols from the fruiting bodies of were obtained using the deep eutectic solvent (DES) extraction method.

DNA Barcoding Mushroom Spawn Using EF-1α Barcodes: A Case Study in Oyster Mushrooms ().

Oyster mushrooms (genus ) are widespread and comprise the most commonly cultivated edible mushrooms in the world. Species identification of oyster mushroom spawn based on cultural, morphological, and cultivated characteristics is time consuming and can be extraordinarily difficult, which has impeded mushroom breeding and caused economic loss for mushroom growers. To explore a precise and concise approach for species identification, the nuclear ribosomal internal transcribed spacer (ITS), 28S rDNA, and the widely used protein-coding marker translation elongation factor 1α (EF-1α) gene were evaluated as candidate DNA barcode markers to investigate their feasibility in identifying 13 oyster mushroom species.