Suppression of Tescalcin inhibits growth and metastasis in renal cell carcinoma via downregulating NHE1 and NF-kB signaling.

Background: Renal cell carcinoma (RCC) is the most common form of kidney cancer. Recent studies reported that Tescalcin was overexpressed in various tumor types. However, the status of Tescalcin protein expression in RCC and its biological function is uncertain.

[Causes of orchiectomy: An analysis of 291 cases].

Objective: To study the causes of orchiectomy in different age groups.

Methods: We retrospectively reviewed the clinical data about 291 cases of orchiectomy performed between March 1993 and October 2014 and analyzed the causes of surgery and their distribution in different age groups.

Results: The main causes of orchiectomy were testicular torsion (45.

[Distribution of HoxA13 in the prepuce and urethral plate in different types of hypospadias and its implication].

Objective: To investigate the role of the HoxA13 gene from the HOX family in the development of hypospadias by detecting the transcription and expression of HoxA13 in the prepuce and urethral plate of hypospadias patients.

Methods: We collected the tissues from the prepuce and urethral plate of 30 hypospadias patients aged 3.3 - 11.

Association between reversal of multidrug resistance by methyl jasmonate and P-glycoprotein ATPase activity in hepatocellular carcinoma.

Objective: To study the effects of methyl jasmonate on multidrug resistance in a mouse model of hepatocellular carcinoma.

Methods: Multidrug resistant H22 (H22/FAP) hepatocellular carcinoma cells were produced in vitro by continuous exposure to increasing doses of doxorubicin, cisplatin and 5-fluorouracil (FAP regimen). Cell toxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolum bromide (MTT) assay.

[Low-dose tadalafil for erectile dysfunction following pelvic fracture-induced urethral injury: clinical observation of 42 cases].

Objective: To evaluate the efficacy of daily low-dose tadalafil in the treatment of erectile dysfunction (ED) following pelvic fracture-induced urethral injury (PFUI).

Methods: Based on the length of time between pelvic fracture and the patients' clinic visit, we divided 42 PFUI-caused ED patients into groups A (< 1 month), B (6-24 months) and C (> 24 months). We treated them with tadalafil at 5 mg daily for 12 weeks consecutively, followed by evaluation of the therapeutic effect using IIEF-5 questionnaire and Sexual Encounter Profile (SEP) diaries.

[Transfection of iNOS suppresses the growth of androgen-independent prostate cancer DU145 cells].

Objective: To observe the effect of the inducible nitric oxide synthase (iNOS) gene on androgen-independent prostate cancer DU145 cells in vitro.

Methods: The iNOS gene was transfected into androgen-independent prostate cancer DU145 cells. The positive cells were selected as the transfected group for amplification, and an empty vector (EV) group and a control group were also set.

[Effects of tadalafil on erectile dysfunction: on-demand versus once-daily dosing].

Objective: To evaluate the effects of tadalafil administered on demand or once a day in the treatment of erectile dysfunction (ED).

Methods: We randomly assigned 61 ED patients to three groups to receive tadalafil on demand, at 5 mg once daily, and at 10 mg once daily, respectively. After 42 days of medication, we compared the therapeutic effects among different groups using the patients' sexual encounter profile (SEP) diaries, detected the adverse reactions and assessed the safety of tadalafil.

[Causes, location and management of BPH surgery-related urethral stricture].

Objective: To analyze the possible causes of BPH surgery-related urethral stricture and summarize the experience in its clinical management.

Methods: The clinical data of 37 cases of BPH surgery-related urethral stricture were analyzed retrospectively. The patients averaged 68.

[Transverse preputial island flap technique (Duckett's procedure) for hypospadias repair: a report of 356 cases].

Objective: To evaluate the effect of the transverse preputial island flap technique (Duckett's procedure) for hypospadias repair.

Methods: A total of 356 patients with hypospadias were treated by Duckett's procedure from March 1995 to December 2010, of whom 324 (91.0%) were younger than 14 years.

SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis.

SUN domain-containing proteins belong to a novel protein family. To date, several members--SUN1, SUN2, SUN3, and SPAG4--have been identified as nuclear envelope (NE) proteins. In this study, we sought to characterize and define the potential function of SPAG4L, a newly identified SUN protein.

[Prokaryotic expression and purification of SPAG4L, a novel human testis gene].

Objective: To express SPAG4L, a novel human testis gene in E. coli and purify it's fusion protein.

Methods: The fragment encoding SPAG4L126-379 was amplified by RT-PCR and the PCR products were cloned into PUCm-T vectors.

[Urethral dilatation with the renal sheath dilator under the ureteroscope to treat male urethrostenosis].

Objective: To explore the efficacy of urethral dilatation with the renal sheath dilator under the ureteroscope in the treatment of male patients with urethrostenosis.

Methods: Eighteen male patients with urethrostenosis underwent urethral dilatation with the renal sheath dilator. Under the ureteroscope, a zebra-guide wire was inserted through the stenosed urethra into the bladder and the stenosis was gradually dilated with the renal sheath dilator, followed by placing a Foley catheter of proper size for 1-4 weeks.

[Molecular cloning of a novel human testis-specific gene TDRG1].

Objective: To clone a novel human testis-specific gene TDRG1.

Methods: A new contig of expression sequence tags (ESTs) Hs.180197 was identified from the testis libraries using digital differential display (DDD) to screen the novel human testis-specific gene.

[Effect of androgen on nitric oxiode synthase activity in the kidney of rats with ischemic injury].

Objective: To investigate the effect of androgen on nitric oxide synthase activity and kidney function in the kidney of rats with ischemic injury.

Methods: Fourty-eight adult healthy male SD rats were randomly divided into the renal ischemia group (n = 24 ) and the orchidectomized group with renal ischemia (n = 24). The two groups were randomly divided into 4 groups by the survival time: 0, 1, 3 and 7 days.

[Erectile dysfunction induced by pelvic fracture urethral injury].

Objective: To explore the aetiology, diagnosis,and treatment of erectile dysfunction (ED) after a pelvic fracture urethral injury.

Methods: Rigiscan and Duplex ultrasound were used to determine the causes of ED induced by pelvic fracture urethral injury in 29 patients and the therapeutic means were also analyzed.

Results: Of the 29 patients, 1 was psychological, 28 were physical, 7 vascular, and 15 neurological.

[Uroflowmetry and its influence factors in benign prostate hyperplasia patients].

Objective: To determine the relationship between uroflowmetry and age, the course of disease, premicturition volume, transition zone index and proportion of stroma-to-epithelium in benign prostate hyperplasia (BPH) patients.

Methods: Eighty-nine BPH patients in our hospital from 2000 to 2003 were evaluated. With the CMM3 pathology image analysis system, transrectal ultrasound and Dantec 2000 urodynamic instrument, the value of influence factors was determined.