[Reviews on natural naphthoquinones and their bioactivities].

The naphthaquinones are widely distributed in plants. They are usually in higher plants, but a few of them were also found in microorganisms. There is a lot of research showing that they had multiple pharmaco-activities such as cytotoxic, antioxidant, anti-inflammatory and antibacterial activities, etc.

[Constituents isolated from n-butanol extract of leaves of Moringa oleifera].

Seventeen compounds were isolated from n-butanol extract of the leaves of Moringa oleifera, using column chromatography over macroporous resin HP-20,Sephadex LH-20, and ODS. Their structures were identified as two carboline,tangutorid E(1) and tangutorid F(2); three phenolic glycosides,niazirin(3)，benzaldehyde 4-O-α-L-rhamnopyranoside(4) and 4-O-β-D-glucopyranosidebenzoic acid(5); four chlorogenic acid and derivatives,4-caffeoylquinic acid(6),methyl 4-caffeoylquinate(7),caffeoylquinic acid(8) and methyl caffeoylquinate(9); two nucleosids,uridine(10) and adenosine(11); one flavone,quercetin 3-O-β-D-glucopyranoside(12); five other types of compounds,phthalimidineacetic acid(13),3-pyridinecarboxamide(14),3,4-dihydroxy-benzoic acid(15),5-hydroxymethyl-2-furancarboxylic acid(16) and 5-hydroxymethyl-2-furaldehyde(17) by the spectral data of ¹H, ¹³C-NMR and MS. Among them,compounds 1-2,7,9-10,16 and 17 were isolated from M.

ABCA1 mRNA expression and cholesterol outflow in U937 cells.

Objective: To investigate the oxidized low density lipoprotein (oxLDL) on U937 cell ATP-binding cassette transporter A1 (ABCA1) mRNA expression and cholesterol efflux situation.

Methods: Human U937 cells were incubated with gradient concentrations of oxLDL (0, 25, 50, 75, 100, 125 mg/L), and then dyed by oil red O to estimate the content of intracelluar lipid and detect the expressing quantity of ABCA1 mRNA by Real-time Fluorescence quantitative PCR simultaneously. Calculating the cholesterol efflux rates by using the scintillation counter to detect the amount of H(3)-cholesterol in each well cell culture plate and medium.

α-Klotho is an acute phase protein and altered by restraint stress in mice.

Objective: To identify whether α-Klotho is a kind of acute phase protein and alternation in the level of protein and mRNA under restraint stress.

Method: 48 mice were divided into three groups of 16: 1h, 10h and 20 h treat time group. Each group included restraint stress and control subgroup with same number animals (n = 8).

Klotho protein lowered in elderly hypertension.

We intend to identify the relationship between Klotho protein and elderly hypertension. Serous Klotho protein and nitric oxide (NO) which gathered from 79 elderly hypertensive patients, 30 elderly non-hypertensive patients and 25 non-elderly hypertensive patients were detected by ELISA and nitro-reductase method, respectively; and the comparison came from the former group and the last two groups. The results were as follows: Klotho protein absorbance (0.

[Effect and mechanism of Danhong injection on isolated mesenteric arterial rings in rats].

Objective: To study the effect and mechanism of Danhong injection on isolated mesenteric arterial rings in rats.

Method: An isolated vascular ring experiment was conducted to determine the changes in tension of vascular rings with a biological signal collection and analytical system.

Result: Danhong injection had no impact on the tension of vascular rings.

[Applications of GIS in biomass energy source research].

Biomass resources have the characteristics of widespread and dispersed distribution, which have close relations to the environment, climate, soil, and land use, etc. Geographic information system (GIS) has the functions of spatial analysis and the flexibility of integrating with other application models and algorithms, being of predominance to the biomass energy source research. This paper summarized the researches on the GIS applications in biomass energy source research, with the focus in the feasibility study of bioenergy development, assessment of biomass resources amount and distribution, layout of biomass exploitation and utilization, evaluation of gaseous emission from biomass burning, and biomass energy information system.

[Single nucleotide polymorphisms of genes associated with high density lipoprotein metabolism in Chinese population].

Objective: To study the single nucleotide polymorphisms in genes associated with the high density lipoprotein (HDL) metabolism in Chinese population.

Methods: Two hundred and nine normal Han ethnic subjects, aged 59+/-10 years, were recruited from 5 medical centers in western part of China. DNA was extracted by proteinase K digestion, phenol and chloroform extraction as well as isopropanol precipitation.