[Evaluation of the trueness and tissue surface adaptation of digital and traditional complete denture bases].

Purpose: To compare the trueness of the tissue surface of the complete denture base among the digital cutting complete denture, the digital 3D printing complete denture and the conventional complete denture.

Methods: Three edentulous patients were selected, and three complete dentures were made for each case. Cutting dentures (cutting group), 3D printed dentures(printing group) were considered as the experimental groups, traditional dentures (traditional group) were considered as the control group.

Bone marrow mesenchymal stem cell-derived exosome uptake and retrograde transport can occur at peripheral nerve endings.

We investigated the occurrence of mesenchymal stem cell (MSC)-derived exosome uptake and retrograde transport at peripheral nerve endings using bone marrow MSCs (bMSCs) transduced with recombinant CD63-green fluorescent protein (GFP) lentiviral plasmid. GFP was used to track the release of bMSC-derived exosomes and the uptake and transport at peripheral nerve terminals, the dorsal root ganglion (DRG), and the spinal cord. cell culture and injection of a CD63-GFP exosome suspension into the right gastrocnemius muscle of an rat model were also performed.

The protective effect and underlying mechanism of Hainan papaya water extract against neuronal apoptosis induced by Aβ40.

Objective: To investigate whether Hainan papayas has protective effects in an Aβ40-induced primary neuron injury model and elucidate the underlying molecular mechanism.

Methods: Cultured primary neurons from the dorsal root ganglia (DRG) of Sprague-Dawley (SD) rats were treated with 20 μM Aβ40 peptide, 100 μg/L Hainan papaya water extract, peptide plus extract, or culture medium for 24 h. Cell viability was measured by MTT assay, and neuronal apoptosis was evaluated by DAPI staining.

[Effect of different surface treatments on the bond strength of fiber posts to resin cement].

Purpose: To evaluate the effects of five different surface treatments on the bond strength between fiber posts and resin cement.

Methods: Fifty fiber posts were randomly divided into 5 groups for different surface treatments. Group A was treated with silane coupling agent (Clearfil Porcelain Bond Activator,Kuraray); Group B was treated with silane coupling agent and then coated with dentin bonding agent (Clearfil SE Bond,Kuraray); Group C was immersed in 30% H(2)O(2); Group D was immersed in 30 % H(2)O(2) and then treated with silane coupling agent; Group E received no surface treatment (control group).

srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons by inactivating RAC1.

Objective: To explore effect of srGAP3 promotes neurite outgrowth of dorsal root ganglion neurons.

Methods: In this study, expression of Slit1 was observed predominantly in the glia, while expression of Robo2 and srGAP3 was detected in sensory neurons of postnatal rat cultured dorsal root ganglion (DRG). Furthermore, upregulation of srGAP3 following sciatic nerve transection was detected by immunohistochemistry and Western blotting.

[Effect of different bonding procedure and root region on bond strength of fiber posts to root canal].

Purpose: To evaluate the effect of different bonding procedure and different root region on the bond strength of fiber posts to root canal.

Methods: Forty-two extracted intact human maxillary central incisors were endodontically treated with post spaces be prepared and divided into 6 groups randomly. The fiber posts were then luted with the following different bonding procedures.

Slit-Robo GTPase-activating proteins are differentially expressed in murine dorsal root ganglia: modulation by peripheral nerve injury.

The Slit-Robo GTPase-activating proteins (srGAPs) play an important role in neurite outgrowth and axon guidance; however, little is known about its role in nerve regeneration after injury. Here, we studied the expression of srGAPs in mouse dorsal root ganglia (DRG) following sciatic nerve transection (SNT) using morphometric and immunohistochemical techniques. Reverse transcriptase polymerase chain reaction and Western blot analysis indicated that srGAP1 and srGAP3, but not srGAP2, were expressed in normal adult DRG.

MicroRNAs 144, 145, and 214 are down-regulated in primary neurons responding to sciatic nerve transection.

MicroRNAs (miRNAs) play an important role in the development, differentiation, proliferation, survival, and oncogenesis of cells and organisms including nervous system. However, the role of miRNAs in primary neurons of dorsal root ganglion (DRG) after injury was not clear. In this study, a miRNA microarray analysis was performed, and a total of 21 miRNAs were found to be down-regulated following unilateral sciatic nerve transection.

[The protective effect of puerarin on Abeta(25-35)-induced PC12 cell injury].

Objective: To study The protective effect of puerarin on Abeta(25-35)-induced PC12 cell injury.

Methods: PC12 cells were treated with puerarin for 0.5 h, then incubated with Abeta(25-35) (50 micromol/L) for 24 h to investigate the production of reactive oxygen species (ROS), mitochondrial membrane potential levels and Caspase-3 activation; The expressions of Bax, bcl-2 were measured by Western Blotting.

[Comparative study of 4 types of luting cements on shear bond strength of Zirconia ceramics to dentin].

Purpose: To investigate the adequate luting cements for zirconia ceramics to dentin.

Methods: Blocks of sintered zirconia ceramics were randomly divided into 4 groups with 8 slices in each. After saliva immersion,airborne-particle abraded ceramic specimens were cleaned with phosphoric acid gel(containing 35% phosphoric acid) and then bonded to dentin with these four kinds of luting cements.

Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor.

Secreted Slit proteins have previously been shown to signal through Roundabout (Robo) receptors to negatively regulate axon guidance and cell migration. During vertebrate development, Slit proteins have also been shown to stimulate branching and elongation of sensory axons and cortical dendrites. In this study, Slit1/Robo2 mRNA and protein expressions were detected in adult rat dorsal root ganglion (DRG) and in cultured DRG neurons.

[Effect of nano-silica coating on bonding strength of zirconia ceramics to dentin].

Purpose: To investigate the effect of silica coating by sol-gel process on bonding strength of zirconia ceramics to dentin.

Methods: Blocks of sintered zirconia ceramics were cut and randomly divided into 4 groups,16 slices in each group. Each group was subject to one of the 4 kinds of surface treatment (control group, sandblasting, sandblasting +silicone, sandblasting + silica coating + silicone) and then bonded to dentin with resin cement.

[Comparative study of bond strength between zirconia ceramics and 4 luting cements].

Purpose: To study the bonding strength of zirconia ceramics with 4 kinds of luting cement materials.

Methods: Blocks of sintered zirconia ceramics were cut and randomly divided into 4 groups with 16 slices in each group. They were treated with sandblasting and bonded with 4 kinds of luting cements respectively.

[Study on abutment movement of distal removable dentures retained by attachments].

Purpose: To study the influence on the abutment movement with three kinds of precision attachments designed for distal-extension removable partial dentures (RPDs).

Methods: Snap attachment, Ceka attachment, Mini-SG attachment were used to retain RPDs. Then food chip was masticated between the upper and lower dentitions on the attachments side.