Tear film instability is associated with weakened colocalization between occludin and MUC5AC in scopolamine-induced dry eye disease (DED) rats.

Purpose: Dry eye disease (DED) is a disease with tear film instability because of multiple factors. This study was conducted to explore roles of occludin and MUC5AC in tear film instability in DED rat model.

Methods: A total of 20 SD rats were divided into DED group (n = 10) and normal control (NC) group (n = 10).

Corallococcus silvisoli sp. nov., a novel myxobacterium isolated from subtropical forest soil.

An orange-pigmented myxobacterium, designated strain c25j21, was isolated from subtropical forest soil collected from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on the 16S rRNA gene and core genes clearly showed that the novel strain was affiliated within the genus Corallococcus and most closely related to Corallococcus aberystwythensis DSM 108846 (99.3% 16S rRNA gene sequence similarity), while C.

sp. nov., isolated from rhizosphere soil of banana.

A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as strain B61, was isolated from rhizosphere soil of banana collected from Dongguan, Guangdong Province, PR China. Growth occurred at 15-40 °C, within a pH range of pH 6.0-9.

sp. nov., isolated from forest soil.

A novel bacterial strain, designated ysch24, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24 belongs to the genus , and was most closely related to GDMCC 1.

sp. nov., isolated from tungsten mine tailing.

A novel pink-pigmented strain, designated 6HR-1, was isolated from tungsten mine tailings in Jiangxi Province, PR China. Cells were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and motile with a polar flagellum (monotrichous). It could not utilize methanol, methylamine, formaldehyde or formate as a sole carbon source.

Transfer of , , and corrig. into the novel genus gen. nov. and into the novel genus gen. nov. within the family .

During a phylogenetic analysis of and its closely related genera in the family , we found that the genus and the species might not be properly assigned in the taxonomy. Phylogenetic, phenotypic and chemotaxonomic characterizations clearly showed that the genus should be reclassified into two genera (Clade I and Clade II), for which the original genus name, , is proposed to be retained only for Clade I, and a new genus named as gen. nov.

and Lee and Jeon 2017 are two later heterotypic synonyms of Chen . 2013 and emended description of the species.

Phylogenetic analysis of the genus had shown that the type strains of , and shared a very close relationship between each other. The 16S rRNA gene sequences similarity values between each other ranged from 99.65 to 99.

sp. nov., isolated from tropical forest soil.

A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15-37 °C and pH 6.0-8.

Hymenobacter metallilatus sp. nov., isolated from abandoned lead-zinc ore.

An aerobic, non-motile, Gram-stain-negative, red-to-pinkish and rod-shaped bacterium, designated 9PBR-2, was isolated from an abandoned lead-zinc ore sample collected from Meizhou, Guangdong Province, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 9PBR-2 belongs to the genus Hymenobacter and was most closely related to Hymenobacter rigui KCTC 12533 (98.0 %), Hymenobacter swuensis KCTC 32018 (97.

Whole genome sequences reveal the presence of 11 heterotypic synonyms in the genus Sphingobium and emended descriptions of Sphingobium indicum, Sphingobium fuliginis, Sphingobium xenophagum and Sphingobium cupriresistens.

Phylogenetic analysis based on 16S rRNA gene sequences of the genus Sphingobium showed the presence of four distinguishable clusters, in each of which the species shared almost the same evolutionary distance. They were Sphingobium indicum, Sphinogbium lucknowense, Sphinogbium chinhatense, Sphinogbium francense and Sphinogbium japonicum in cluster I, Sphinogbium barthaii and Sphinogbium fuliginis in cluster II, Sphinogbium hydrophobicum and Sphinogbium xenophagum in cluster III and Sphinogbium czechense and Sphinogbium cupriresistens in cluster IV. The 16S rRNA gene sequence similarities between the species in each cluster were all higher than 98 %.

Sphingomonas lenta sp. nov., a slowly growing bacterium isolated from an abandoned lead-zinc mine.

A novel slowly growing member of the genus Sphingomonas, designated 1PNM-20, was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, PR China. A polyphasic taxonomic study was performed to characterize the novel strain. Growth occurred on Reasoner's 2A (R2A) agar and peptone-yeast extract (PYE) agar, but not in liquid R2A or PYE media.

Microvirga flavescens sp. nov., a novel bacterium isolated from forest soil and emended description of the genus Microvirga.

An aerobic, Gram-stain-negative, motile and rod-shaped bacterium, designated c27j1, was isolated from a forest soil sample from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain c27j1 belongs to the genus Microvirga and was most closely related to Microvirga aerophila DSM 21344 (97.7 %) and Microvirga subterranea DSM 14364 (96.

Lysobacter silvisoli sp. nov., isolated from forest soil.

A yellow-pigmented, Gram-stain-negative, gliding and rod-shaped bacterial strain, designated zong2l5, was isolated from a forest soil sample at Dinghu Mountain, Guangdong Province, PR China. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain zong2l5 belongs to the genus Lysobacter, and was most closely related to Lysobacter enzymogenes KCTC 12131 (97.7 %) and Lysobacter soli KCTC 22011 (97.

Chitinophaga varians sp. nov., isolated from forest soil.

A Gram-stain-negative, aerobic, non-motile bacterium, designated strain 10-7W-9003, was isolated from the forest soil of Limushan National Forest Park, south-east China (19° 10' 42″ N, 109° 44' 45″ E). Strain 10-7W-9003 showed a shape change during the course of culture from long filamentous cells (5-10×0.4-0.

A high ratio of dietary n-3/n-6 polyunsaturated fatty acids improves obesity-linked inflammation and insulin resistance through suppressing activation of TLR4 in SD rats.

Dietary ratios of n-3/n-6 polyunsaturated fatty acids (PUFAs) have been implicated in controlling markers of metabolic disorders, including obesity, insulin resistance (IR), inflammation, and lipid profiles, which are also presumed to be partly related to type 2 diabetes mellitus (T2DM). However, molecular mechanisms of the different PUFAs related to metabolic disorders have not been systematically addressed. The present study aimed to investigate the impact of dietary n-3/n-6 PUFA ratios on obesity and IR and, further, to determine the underlying mechanisms.