Effects of Cu(Ⅱ) stress on embryonic development, oxidative stress response, cholinergic system and energy metabolism in the Sepiella maindroni.

In order to investigate the causes of population degradation and resource decline, this thesis investigated the ecotoxicological effects of heavy metal Cu(Ⅱ) on the embryonic development of Sepiella maindroni. Results indicate significant effects of Cu(Ⅱ) concentrations on the developmental toxicity, teratogenicity, and lethality of S. maindroni embryos.

Transcriptome analysis of Sesuvium portulacastrum L. uncovers key genes and pathways involved in root formation in response to low-temperature stress.

Sesuvium portulacastrum L., a perennial facultative halophyte, is extensively distributed across tropical and subtropical coastal regions. Its limited cold tolerance significantly impacts both the productivity and the geographical distribution of this species in higher-latitude areas.

Physiological response and tolerance of L. to low temperature stress.

Unlabelled: The plant L., commonly referred to as sea purslane, is a perennial halophytic species with significant potential for development in marine ecological restoration. However, its growth is limited in high-latitude regions with lower temperatures due to its subtropical nature.

Multidimensional Frailty Instruments Can Predict Acute Exacerbations Within One Year in Patients with Stable Chronic Obstructive Pulmonary Disease: A Retrospective Longitudinal Study.

Background: Chronic obstructive pulmonary disease (COPD) is closely associated with frailty, and prevention of acute exacerbations is important for disease management. Moreover, COPD patients with frailty experience a higher risk of acute exacerbations. However, the frailty instruments that can better predict acute exacerbations remain unclear.

Asymmetric Synthesis of Chiral 1,2-Bis(Boronic) Esters Featuring Acyclic, Non-Adjacent 1,3-Stereocenters.

The construction of acyclic, non-adjacent 1,3-stereogenic centers, prevalent motifs in drugs and bioactive molecules, has been a long-standing synthetic challenge due to acyclic nucleophiles being distant from the chiral environment. In this study, we successfully synthesized highly valuable 1,2-bis(boronic) esters featuring acyclic and nonadjacent 1,3-stereocenters. Notably, this reaction selectively produces migratory coupling products rather than alternative deborylative allylation or direct allylation byproducts.

Effects of low temperature and highlight stress on lipid accumulation and cell structure of Tropidoneis maxima.

Tropidoneis maxima is a marine diatom with a rapid growth rate that produces high levels of lipids. To explore whether the lipid content could be further enhanced, cultures were first incubated under optimal conditions and then stressed under low temperature (10°C), a high light intensity level (80 μmol/m ·s), and the two factors together (interaction treatment). The results indicated that high light intensity and the temperature-light interaction exhibited greater impacts on lipid synthesis of T.

Combined metabolomics and histological analysis of the tissues from cuttlefish Sepia pharaonis exposed to inking stress.

Inking is part of a defensive stress response in cephalopods (cuttlefish, squid, and octopus). Some individual cuttlefish (Sepia pharaonis) die after continued stress and inking; however, the physiological effects of cephalopods in response to stress and inking remain unknown. The present study investigated the metabolic profile and discussed the physiological roles of S.

H NMR-based metabolomic analysis of cuttlefish, Sepia pharaonis (Ehrenberg, 1831) exposed to hypoxia stresses and post-anoxia recovery.

Oxygen deficiency (hypoxia and anoxia) is an emerging concern in estuarine and coastal ecosystems worldwide. Previous studies on Mollusca Cephalopoda have focused on the effects of hypoxia stress on physiological performance and survival, but there are few reports on the molecular mechanism, and the application of metabolomics in cephalopods remains unknown. In this study, a H nuclear magnetic resonance (NMR) based metabolomics approach was applied to investigate the metabolites profiles of Sepia pharaonis (Ehrenberg, 1831) during hypoxia and post-anoxia recovery.

Continuous Inking Affects the Biological and Biochemical Responses of Cuttlefish .

Several marine mollusks, including cephalopods (cuttlefish, squid, and octopus) and gastropods (e.g., sea hares), can release a colored ink secretion when chased by predators or stimulated.

A Visible-Light-Induced Dynamic Mechanical Bond as a Linkage for Dynamic Materials.

Exploring dynamic bonds and their applications in fabricating dynamic materials has received great attention. A photoinduced [2]rotaxane-based dynamic mechanical bond (DMB) features visible-light-triggered dynamic bonding behavior that is essentially distinguished from conventional dynamic chemical bonds. In this DMB, a photoisomerizable ortho-fluoroazobenzene unit is introduced as a steric-controllable stopper, the visible-light-induced dynamic wagging movement of which enables the photoregulated threading of the macrocycle.

Isolation and characterization of a virulence related Vibrio alginolyticus strain Wz11 pathogenic to cuttlefish, Sepia pharaonis.

Vibrio alginolyticus is a ubiquitous marine opportunistic pathogen that can infect various hosts in marine environment. In the present study, V. alginolyticus strain Wz11 was isolated from diseased cuttlefish, Sepia pharaonis, with 20% of promoted death and high survival capacity in skin mucus and tissue liquid.

Metabolic responses and arginine kinase expression of juvenile cuttlefish (Sepia pharaonis) under salinity stress.

The pharaoh cuttlefish Sepia pharaonis is particularly sensitive to environmental changes in its breeding environment. The breeding of S. pharaonis larvae was carried out in different salinities for 48h, and the changes in survival rate, histological structure, energy metabolism, and anti-oxidative stress parameters were investigated and correlated with arginine kinase (AK) expression changes in muscle and liver tissues.

Kinetics for Zinc Ion Induced Sepia Pharaonis Arginine Kinase Inactivation and Aggregation.

Arginine kinase is an essential enzyme which is closely related to energy metabolism in marine invertebrates. Arginine kinase provides a significant role in quick response to environmental change and stress. In this study, we simulated a tertiary structure of Sepia pharaonis arginine kinase (SPAK) based on the gene sequence and conducted the molecular dynamics simulations between SPAK and Zn(2+).

[Enrichment of Cd2+ and Hg2+ in Phascolosoma esculenta and their effects].

The accumulation of Cd2+ and Hg2+ in Phascolosoma esculenta and its effects on its growth and main nutrient components were investigated using atomic absorption spectrometer analysis and atomic fluorescence spectrometer examination. The results showed that within the experimental heavy metal concentrations, the amounts of Cd2+ and Hg2+ accumulated in the somatic muscles increased with increasing exposure time and reached their saturated levels at the end of the experiment. Exposure of higher heavy metal concentrations speeded up the accumulation of Cd2+ and Hg2+ and subsequently took less time to reach their saturated levels, which were greater than those of lower concentration groups.

Conditions optimising on the yield of biomass, total lipid, and valuable fatty acids in two strains of Skeletonema menzelii.

Polyunsaturated fatty acids (PUFA) and eicosapentaenoic acid (EPA) are essential for the health of aquaculture organisms and human beings. A total of 9 species of diatoms were screened and two strains of Skeletonema menzelii were selected for further study due to their high growth rates, PUFA and EPA contents. The culture conditions for the yield of biomass, total lipid, EPA and PUFA were optimised.

Effects of cadmium on the cuttlefish Sepia pharaonis' arginine kinase: unfolding kinetics integrated with computational simulations.

Arginine kinase is closely associated with adaptation to environmental stresses such as high salinity and heavy metal ion levels in marine invertebrates. In this study, the effects of Cd(2+) on the cuttlefish Sepia pharaonis' arginine kinase (SPAK) were investigated. SPAK was isolated from the muscles of S.

Complete mitochondrial genome of Gnathopogon taeniellus (Cypriniformes: Cyprinidae).

The complete mitochondrial genome of Gnathopogon taeniellus was first determined in this study. It is 16,596 bp in length, contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and one control region in the typical vertebrate gene order and transcriptional direction. There are total of 29 bp short overlaps and 34 bp non-coding intergenic spacers were found in the mitogenome.

A virtual screening method for inhibitory peptides of Angiotensin I-converting enzyme.

Natural small peptides from foods have been proven to be efficient inhibitors of Angiotensin I-converting enzyme (ACE) for the regulation of blood pressure. The traditional ACE inhibitory peptides screening method is both time consuming and money costing, to the contrary, virtual screening method by computation can break these limitations. We establish a virtual screening method to obtain ACE inhibitory peptides with the help of Libdock module of Discovery Studio 3.

[Effects of temperature on the embryonic development and larval growth of Sepia lycidas].

A single-factor experiment was conducted to study the effects of different temperature (15, 18, 21, 24, 27, 30, and 33 degrees C) on the embryonic development and larval growth of Sepia lycidas, aimed to search for the optimum temperature for the development and growth of S. lycidas. The results showed that temperature had significant effects on the embryonic development and larval growth of S.

[Effects of several ecological factors on the hatching of Sepiella maindroni wild and cultured eggs].

This paper compared the differences between Sepiella maindroni wild and cultured eggs, and studied the effects of temperature, salinity, hatching density, and egg type on the hatching rate and hatching time of the eggs. Wild eggs had better quality than cultured eggs. Among cultured eggs, small and black eggs had the best quality.

[Oxygen consumption rate and effects of hypoxia stress on enzyme activities of Sepiella maindron].

The oxygen consumption rate and suffocation point of Sepiella maindroni were determined through the measurement of dissolved oxygen in control and experimental respiration chambers by Winkler's method, and the changes of S. maindroni enzyme activities under different levels of hypoxia stress were studied. The results indicated that the oxygen consumption rate of S.

[Effects of diet condition on survival and metamorphosis of Charybdis japonica larvae].

This paper studied the effects of different kind and density of baits and different starvation duration on Charybdis japonica larvae. The results showed that the preferable baits of zoaea-1 were Chaetoceros sp. and Isochrysis galbana, and their proper feeding density were 20 x 10(4) cell.