Characterization of metabolic profile of Dazhu Hongjingtian and evaluation of its anti-hypoxic constituents.

Dazhu Hongjingtian (DZ) is renowned for its diverse pharmacological activities, yet its metabolic pathways remain to be fully elucidated. In this study, the metabolic profile after oral administration of DZ extract (DZE) in rats was systematically identified by the UPLC/Q-TOF-MS/MS method for the first time. A total of 94 components, including 32 prototypes and 62 metabolites, were tentatively characterized in rat plasma and various tissues samples.

Identification of the absorptive constituents and their metabolites in vivo of Ziziphi Spinosae Semen by UPLC-ESI-Q-TOF-MS/MS.

In this research, ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) was used for detection and identification of the absorptive constituents and their metabolites in rat plasma, urine and feces following oral administration of Ziziphi Spinosae Semen alcohol extract. After structure elucidation, a total of 12 compounds in rat plasma, comprising seven prototypes and five metabolites, 28 compounds in urine, comprising 17 prototypes and 11 metabolites, and 23 compounds in feces, comrpising 17 prototypes and six metabolites, have been tentatively identified by comparison with standard compounds and reference literature information. To the best of our knowledge, this is the first comprehensive and systematical metabolic study on the seed.

[¹H-NMR quantitative analysis and fingerprints of ginkgo diterpene lactone raw material].

Ginkgo diterpene lactone raw material, as a raw material for ginkgo diterpene lactone meglumine injection, is extracted and purified from ginkgo leaf. ¹H-NMR content determination method and fingerprint analysis method were respectively established for ginkgo diterpene lactone raw material. Content determination was conducted in 3 batches of samples by using ¹H-qNMR, and then the results were basically consistent with the results in HPLC method.