Association of IGF1R polymorphisms (rs1546713) with susceptibility to age-related cataract in a Han Chinese population.

Aim: To explore the susceptible association between the insulin-like growth factor-1 receptor (IGF1R) single nucleotide polymorphism (SNP) and age-related cataract (ARC), and investigate the underlying mechanisms in human lens epithelium (HLE) cells.

Methods: Totally 1190 unrelated participants, comprising 690 ARC patients and 500 healthy individuals in Han Chinese population were recruited and genotyped for target SNP. The -test was used to detect genotypic distribution between the patient and control groups and the logistic regression was performed to adjust the age and gender.

The impact of SNPs on susceptibility to age-related cataract.

Aim: To determine the association of () gene tag single-nucleotide polymorphisms (SNPs) with susceptibility to age-related cataract (ARC).

Methods: In total, 486 ARC patients were matched with 500 healthy controls. All the participants underwent complete ophthalmic examinations.

Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide.

Aim: To explore the effect of parthenolide on hydrogen peroxide (HO)-induced apoptosis in human lens epithelial (HLE) cells.

Methods: The morphology and number of apoptotic HLE cells were assessed using light microscopy and flow cytometry. Cell viability was tested by MTS assay.

Overexpression of GOLPH3 is associated with poor prognosis and clinical progression in pancreatic ductal adenocarcinoma.

Background: Golgi phosphoprotein 3 (GOLPH3) has been identified as an oncoprotein in various human cancers; however, its role in pancreatic ductal adenocarcinoma (PDAC) is unknown. We examined GOLPH3 expression levels and relationship with survival in patients with PDAC to establish the significance of GOLPH3 in the development and progression of PDAC.

Methods: Real-time qPCR and Western blotting were performed to analyze the expression levels of GOLPH3 mRNA and protein in paired PDAC tumor and adjacent non-tumor tissues.

Ocular findings in syndromic gingival fibromatosis: a case study and electronic microscopic investigation of lens.

We report a case of syndromic gingival fibromatosis with notable ocular lesions, bilateral congenital cataracts, esotropia, and high myopia of a 21-year-old male patient from China. The patient was diagnosed with gingival fibromatosis based on his massive gingival overgrowth and histological findings that were consistent with gingival fibromatosis through a gingival biopsy. Lens opacity features were presented and phacoemulsificaion with intraocular lens(IOL) implantation was performed to manage the cataracts in both eyes.

Comparison of outcomes between overlapping-spot and single-spot photodynamic therapy for circumscribed choroidal hemangioma.

Aim: To compare the efficacy and safety of photodynamic therapy (PDT) with overlapping multiple spots and single spot for treating circumscribed choroidal hemangioma.

Methods: Twenty-two patients (22 eyes) with symptomatic circumscribed choroidal hemangioma received PDT treatment. Fourteen patients received overlapping spots (two to three spots) PDT, whereas eight patients received single-spot PDT.

[Mechanism underlying spatial vision deficit of monocular amblyopia based on the theory of Magnocellular and Parvocellular (M-P) pathways].

Objective: To investigate the relationship between the characteristics of spatial vision deficit and the degree of amblyopia in monocular amblyopes, and to analyze its mechanism with the theory of Magnocellular and Parvocellular pathways.

Methods: One hundred and eleven patients with monocular amblyopes aged 7-34 were included in this study. Distance best corrected visual acuity (BCVA) in logMAR units and contrast sensitivity function test were performed on both eyes in all patients with ETDRS digital visual chart and functional test system OPTECR 6500.

Novel mutation c.980_983delATTA compound with c.986C>A mutation of the FRMD7 gene in a Chinese family with X-linked idiopathic congenital nystagmus.

Objective: To screen mutations in FERM domain-containing protein 7 (FRMD7) gene in two Chinese families with X-linked idiopathic congenital nystagmus (XLICN).

Methods: Common ophthalmic data and peripheral blood of two Chinese XLICN families (families A and B) were collected after informed consent. Genomic DNA was prepared from the peripheral blood of members of the two families and from 100 normal controls.

[Clinical evaluation on the coaxial 1.8 mm microincision cataract surgery].

Objective: To study and compare the outcomes of coaxial 1.8 mm microincision phacoemulsification with conventional coaxial 3 mm small-incision cataract surgery.

Methods: A randomized prospective study was conducted on 89 patients with age-related cataract: coaxial 1.

[Study of clinical application of aberration-free aspheric intraocular lens].

Objective: To compare spherical aberration, visual quality and apparent accommodation between pseudophakic patients with an aberration-free aspheric intraocular lens (IOL) and patients with a spherical IOL.

Methods: A prospective study of 130 consecutive cases (132 eyes) was conducted. All cataract patients underwent phacoemulsification were randomized to receive an aberration-free aspheric IOL (Akreos AO, Bausch & Lomb) or a spherical IOL (KS-1, Cannon Staar).

[Computer construction and analysis of protein models of mutant fibrillin-1 gene in Marfan's syndrome].

Objective: Fibrillin-1, the major constituent of extracellular microfibrils, plays an important role in the molecular pathogenesis of Marfan syndrome (MFS, #54700). The aim of this study was to analyze protein models of the mutation of the fibrillin-1 (FBN1) gene on Arg545Cys and Arg1530Cys which have been reported to cause predominant ectopia lentis in Chinese patients.

Methods: We constructed and analyzed the protein models of the mutant FBN1 gene on Arg545Cys and Arg1530Cys.

Protective effect of magnolol against hydrogen peroxide-induced oxidative stress in human lens epithelial cells.

Oxidative stress plays a significant role in the progression of cataract. We aimed to investigate the protective effect of magnolol, a compound extracted from the Chinese herb Magnolia officinalis, against oxidative stress in human lens epithelial (HLE) cells as well as the possible molecular mechanism involved. In this study, magnolol was observed to protect against H2O2-induced cytotoxicity in HLE B-3 cells.

[Clinical evaluation on the bimanual microincision cataract surgery].

Objective: To compare the outcomes of bimanual microincision phacoemulsification with conventional small incision cataract surgery.

Methods: A randomized prospective study of 280 consecutive cases (280 eyes) was conducted. All patients were randomly assigned to receive bimanual microincision cataract surgery (MICS group) or small incision cataract surgery (SICS group).

[Hydrogen peroxide (H2O2) and methyl-beta-cyclodextrin (MPCD) down regulate caveolin expression in human lens epithelial cells (HLECs)].

Oxidative stimulation induced by hydrogen peroxide (H2O2) on human epithelial cells (HLECs) was performed to observe the effects on cell viability, caveolin expression, and cholesterol depletion in HLECs caused by methyl-beta-cyclodextrin (MbetaCD) was also studied. SRA01/04 HLECs were exposed to H2O2 or MbetaCD of various concentrations and durations. We used a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to measure the effect of H2O2 on the viability of SRA01/04 HLECs.

Involvement of PI3K/Akt pathway in TGF-beta2-mediated epithelial mesenchymal transition in human lens epithelial cells.

Background: Epithelial mesenchymal transition (EMT) of postoperative remnants of lens epithelial cells (LECs) can lead to posterior capsule opacification. This study was designed to determine the effect of signaling pathways that contribute to TGF-beta2-mediated EMT in human lens epithelial B-3 cells (HLEB-3 cells).

Methods: The HLEB-3 cells were cultured and stimulated with TGF-beta2 at different concentrations for an indicated time.

[Clinical comparison study of aspheric and spherical intraocular lenses].

Objective: To compare spherical aberration and visual quality in pseudophakic patients with a modified prolate aspheric intraocular lens (IOL) with patients with a spherical IOL.

Methods: A prospective study of 169 consecutive cases (169 eyes) was conducted. All cataract patients underwent phacoemulsification were randomized to receive an aspheric IOL or a spherical IOL.

Integrin beta1-mediated signaling is involved in transforming growth factor-beta2-promoted migration in human lens epithelial cells.

Purpose: Transforming growth factor-beta 2 (TGF-beta2) is a potent growth inhibitor and apoptosis inducer. However, typically TGF-beta2 loses its growth-inhibitory and apoptosis-inducing effects but stimulates the migratory capacity of epithelial cells. In this study, we investigate the possible involvement of integrin and integrin-mediated signaling in TGF-beta2-promoted migration and adhesion in human lens epithelial cells.

[Experimental study on the effects of hydrogen peroxide on caveolin in human lens epithelial cells].

Objective: To observe the expression and distribution of caveolin and phosphorylated caveolin-1 in human lens epithelial cells (HLECs) under H(2)O(2) treatment.

Methods: HLECs (SRA01/04) were exposed to different concentrations of H(2)O(2) for different periods of time. The distribution of caveolin and phosphorylated caveolin-1 in H(2)O(2) treated cells was observed by laser scanning confocal microscopy and fluorescence microscopy.

[Clinical analysis of 60 cases with malignant gastrointestinal stromal tumors].

Objective: To investigate the clinicopathological characteristics, and treatment of malignant gastrointestinal stromal tumors (MGIST).

Methods: Immunohistochemistry was used to detect CD117, CD34, S100, vimentin and SMA expressions. The postoperative curative effect was compared between the patients with or without imatinib treatment.

[Expression of heat shock protein 70 and heat shock protein 27 in lens epithelial cells induced by contusion and thermotolerance in rat model].

Objective: To study the dynamic expression of heat shock protein (HSP) 70 and HSP27 in lens epithelial cells (LECs) in contused eyes of rat model and to detect the dynamic change of the expression of HSP70 and HSP27 after thermotolerance.

Methods: Forty-eight Sprague-Dawley (SD) rats were randomized into two groups: contusion group and thermotolerance group. Contusion eyes were induced by dropping a steel ball of 20 g from the height of 20 cm for 100 times.