Studies on Quality Deterioration and Metabolomic Changes in Oysters Induced by Spoilage Bacteria During Chilled Storage.

The correlation between spoilage bacteria and the degradation of aquatic food quality during chilled storage is substantial. However, our understanding of the precise roles of spoilage bacteria in oyster spoilage remains incomplete. The aim of this study was to explore the role of three dominant spoilage bacteria strains in oyster spoilage.

In situ growth of defective ZIF-8 on TEMPO-oxidized cellulose nanofibrils for rapid response release of curcumin in food preservation.

Uncontrolled release of active agents in active packaging reduces antimicrobial efficacy, hindering the effective protection of perishable products from microbial infection. Herein, a novel defective engineering was proposed to design defective and hollow ZIF-8 structures grown on TEMPO oxidized cellulose nanofibrils (TOCNFs) and use them as fast-reacting nanocarriers for loading and controlled release curcumin (Cur) in sodium alginate (SA) active packaging systems (CZT-Cur-SA). By employing stable chelation between tannic acid (TA) and ZIF-8 zinc ions, the connections between zinc ions and imidazole ligands were severed to form a loose and hollow structure, which facilitates the rapid reaction and release of active ingredients triggered by pH changes in the microenvironment.

Conference 2024: Building an innovative scientific research ecosystem for microbiome and One Health.

The Conference 2024 provides a platform to promote the development of an innovative scientific research ecosystem for microbiome and One Health. The four key components - Technology, Research (Biology), Academic journals, and Social media - form a synergistic ecosystem. Advanced technologies drive biological research, which generates novel insights that are disseminated through academic journals.

Structurally robust chitosan-based active packaging film by Pickering emulsion containing tree essential oil for pork preservation.

The unstable structure of Pickering emulsion caused the fast release of active substance from active packaging and failure food preservation. Herein, a novel in-situ condensation strategy was proposed to construct sustained released chitosan (CS)-based active packaging film, in which the soybean separation protein (SPI)-carboxymethyl cellulose (CMC) emulsion (SCCE) containing tea tree essential oil (TTO) was physically incorporated into CS matrix. Originating from the strong electrostatic interaction of negatively charged SPI-CMC emulsion and positively charged CS matrix, a robust shell was in-situ formed on the outermost layer and served as armor to boost the structural stability of emulsion.

Antibacterial Activity of Phloretin Against and Its Application in Seafood.

Although phloretin is widely utilized in the food industry as an additive, its effects on foodborne pathogens remain insufficiently investigated. This study aimed to evaluate the antimicrobial properties of phloretin (PHL) against () and to elucidate the potential mechanisms of action. After PHL treatment, alterations in the cell morphology, cell microstructure, and intracellular contents of were assessed.

m6A methylation in myocardial tissue of septic mice analyzed using MeRIP/m6A-sequencing and RNA-sequencing.

Septic cardiomyopathy is a secondary myocardial injury caused by sepsis. N6-methyl-adenosine (m6A) modification is involved in the pathological progression of septic cardiomyopathy; however, the pathological mechanism remains unclear. In this study, we identified the overall m6A modification pattern in septic myocardial injury and determined its potential interactions with differentially expressed genes (DEGs).

Gastrodin Alleviates DSS-Induced Colitis in Mice through Strengthening Intestinal Barrier and Modulating Gut Microbiota.

Inflammatory bowel diseases (IBDs) are commonly associated with dysfunctional intestinal barriers and disturbed gut microbiota. Gastrodin, a major bioactive ingredient of Blume, has been shown to exhibit anti-oxidation and anti-inflammation properties and could mitigate non-alcoholic fatty liver disease, but its role in modulating IBD remains elusive. The aim of this study was to investigate the impact of gastrodin on DSS-induced colitis in mice and explore its potential mechanisms.

Total Protein Determination by Recovery Fluorescence of Trinitrotoluene-Quenched Protein-Functionalized Gold Nanoclusters.

Determination of total protein content is very important for clinical, pharmaceutical and food chemistry, and the simplicity and rapidity of the existing total protein content assays should be improved. Here, a novel fluorescent sensor for total protein content measurement was described using near-infrared emitting fluorescent gold nanoclusters, which were prepared with egg whites. Trinitrotoluene served as highly efficient quencher to quench the fluorescence of gold nanoclusters.

Alginate oligosaccharide improves 5-fluorouracil-induced intestinal mucositis by enhancing intestinal barrier and modulating intestinal levels of butyrate and isovalerate.

Chemotherapy-induced mucositis (CIM) is the typical side effect of chemotherapy. This study investigates the potential of alginate oligosaccharide (AOS) in ameliorating CIM induced by 5-fluorouracil (5-FU) in a murine model and its underlying mechanisms. AOS effectively mitigated body weight loss and histopathological damage, modulated inflammatory cytokines and attenuated the oxidative stress.

The role of DsbA and PepP genes in the environmental tolerance and virulence factors of Cronobacter sakazakii.

Cronobacter sakazakii, an opportunity foodborne pathogen, could contaminate a broad range of food materials and cause life-threatening symptoms in infants. The bacterial envelope structure contribute to bacterial environment tolerance, biofilm formation and virulence in various in Gram-negative bacteria. DsbA and PepP are two important genes related to the biogenesis and stability of bacterial envelope.

Intestinal linoleic acid contributes to the protective effects of against infection in mice.

pretreatment mitigated infection in mice. improved gut microbiota disturbed by infection and significantly increased the level of intestinal linoleic acid in mice. Linoleic acid strengthened the intestinal epithelial barrier and reduced pathogen translocation partly by regulating NF-κB/MLCK pathway in a GPR40-dependent manner.

Gut microbiota and D-ribose mediate the anti-colitic effect of punicalagin in DSS-treated mice.

: Inflammatory bowel disease (IBD) is an increasing health burden worldwide. Punicalagin, a bioactive component rich in pomegranate rind, has been shown to attenuate chemical or bacteria-induced experimental colitis in mice, but whether punicalagin exerts its function through modulating gut microbiota and metabolites remains unexplored. : Punicalagin (100 mg per kg per day) administered orally to mice alleviated dextran-sodium sulfate (DSS)-induced colitis.

Haematococcus pluvialis polysaccharides improve microbiota-driven gut epithelial and vascular barrier and prevent alcoholic steatohepatitis development.

Algal polysaccharides possess many biological activities and health benefits, such as antioxidant, anti-tumor, anti-coagulant, and immunomodulatory potential. Gut microbiota has emerged as one of the major contributor in mediating the health benefits of algal polysaccharides. In this study we showed that Haematococcus pluvialis polysaccharides (HPP) decreased serum transaminase levels and hepatic triglyceride content, alleviated inflammation and oxidative stress in the liver of chronic and binge ethanol diet-fed mice.

Mechanically robust carboxymethyl cellulose/graphene oxide composite cross-linked by polyetherimide for fruits packaging and preservation system.

Modulating the interactions between biopolymer matrix and nanofillers highly determined the mechanical performances of composite packaging materials. Herein, we innovatively proposed a sort of eco-friendly and mechanically robust carboxymethyl cellulose/graphene oxide/tannic acid/polyetherimide (CMC/GO/TA/PEI, CGTP) composite by employing PEI as cross-linker and TA as proton donor. The amidation reaction between -NH and -COOH chemically connected the CMC/GO, CMC/CMC and GO/GO and the physical interaction (e.

Antimicrobial Activity and Mechanisms of Punicalagin against .

This study sought to explore the antimicrobial activity of punicalagin against and its potential modes of action. ATCC 17802 and RIMD 2210633 were exposed to punicalagin, and the energy production, membrane potential, and envelope permeability, as well as the interaction with cell biomolecules, were measured using a variety of fluorescent probes combined with electrophoresis and Raman spectroscopy. Punicalagin treatment disrupted the envelope integrity and induced a decrease in intracellular ATP and pH.

The role of rcpA gene in regulating biofilm formation and virulence in Vibrio parahaemolyticus.

Vibrio parahaemolyticus (V. parahaemolyticus) is a common seafood-borne pathogen that can colonize the intestine of host and cause gastroenteritis. Biofilm formation by V.

An improved RIME optimization algorithm for lung cancer image segmentation.

Lung cancer is a prevalent form of cancer worldwide, necessitating early and accurate diagnosis for successful treatment. Within medical imaging processing, image segmentation plays a vital role in medical diagnosis. This study applies swarm intelligence algorithms to segment lung cancer pathological images at three levels.

Saponin Prevents Immune Dysfunction and Strengthens Intestinal Mucosal Barrier Function in Cyclophosphamide-Induced Immunosuppressed BALB/c Mice.

The aim of this study was to explore the immunomodulatory effect of saponin (PS) in a cyclophosphamide-induced (Cy) immunosuppression mice model. Oral administration of PS by gavage effectively alleviated weight loss caused by Cy and increased the index of immune organs. PS promoted the proliferation of splenic lymphocytes and T cell subsets (CD3, CD355, CD4/CD8) and relieved the xylene-induced inflammatory response and Cy-induced increase of serum hemolysin.

YAP1-induced RBM24 promotes the tumorigenesis of triple-negative breast cancer through the β-catenin pathway.

Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype and refractory to current treatments. RBM24 is an RNA-binding protein and shows the ability to regulate tumor progression in multiple cancer types. However, its role in TNBC is still unclear.

Assessing the causal relationship between 731 immunophenotypes and the risk of lung cancer: a bidirectional mendelian randomization study.

Background: Previous studies have observed a link between immunophenotypes and lung cancer, both of which are closely associated with genetic factors. However, the causal relationship between them remains unclear.

Methods: Bidirectional Mendelian randomization (MR) was performed on publicly available genome-wide association study (GWAS) summary statistics to analyze the causal relationships between 731 immunophenotypes and lung cancer.

Design and characterization of edible chitooligosaccharide/fish skin gelatin nanofiber-based hydrogel with antibacterial and antioxidant characteristics.

Antibacterial and active packaging materials have gained significant research attention in response to the growing interest in food packaging. In this investigation, we developed hydrogel packaging materials with antibacterial and antioxidant properties by incorporating chitooligosaccharide (COS) and fish skin gelatin (FSG) nanofiber membranes, which readily absorbed water and exhibited swelling characteristics. The nanofiber membranes were fabricated by electrospinning technology, embedding COS within FSG, and subsequently crosslinked through the Maillard reaction facilitated by the addition of glucose.

CpxAR two-component system contributes to virulence properties of Cronobacter sakazakii.

Cronobacter sakazakii (C. sakazakii) is a foodborne pathogen which threaten susceptible hosts including infants. CpxA/CpxR, a regulatory two-component system (TSC), contributes to stress response and virulence in various Gram-negative pathogens, but its role in C.

Anti-Biofilm Activity of Laurel Essential Oil against .

is a primary seafood-associated pathogen that could cause gastroenteritis. It can attach to various surfaces and form a biofilm, which poses serious threats to food safety. Hence, an effective strategy is urgently needed to control the biofilm formation of .