Untargeted metabolomics analysis as a potential screening tool for 3-methylglutaconic aciduria syndromes.

The 3-methylglutaconic aciduria (3-MGA-uria) syndromes comprise a heterogeneous group of inborn errors of metabolism defined biochemically by detectable elevation of 3-methylglutaconic acid (3-MGA) in the urine. In type 1 (or primary) 3-MGA-uria, distal defects in the leucine catabolism pathway directly cause this elevation. Secondary 3-MGA-uria syndromes, however, are unrelated to leucine metabolism-specific defects but share a common biochemical phenotype of elevated 3-MGA.

The gut-brain axis underlying hepatic encephalopathy in liver cirrhosis.

Up to 50-70% of patients with liver cirrhosis develop hepatic encephalopathy (HE), which is closely related to gut microbiota dysbiosis, with an unclear mechanism. Here, by constructing gut-brain modules to assess bacterial neurotoxins from metagenomic datasets, we found that phenylalanine decarboxylase (PDC) genes, mainly from Ruminococcus gnavus, increased approximately tenfold in patients with cirrhosis and higher in patients with HE. Cirrhotic, not healthy, mice colonized with R.

Protein serine/threonine phosphatases in tumor microenvironment: a vital player and a promising therapeutic target.

The tumor microenvironment (TME) is involved in cancer initiation and progression. With advances in the TME field, numerous therapeutic approaches, such as antiangiogenic treatment and immune checkpoint inhibitors, have been inspired and developed. Nevertheless, the sophisticated regulatory effects on the biological balance of the TME remain unclear.

Isolation and identification of a novel strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by pv. .

Bacterial canker is a devastating disease in kiwifruit production, primarily caused by pv. . In this study, a strain of named JIN4, isolated from a kiwifruit branch, showed antagonistic activity.

Harnessing acylhydrazone-oxime exchange reaction to achieve diverse synthesis of glycosite-specific antibody-drug conjugates.

Glycosite-specific antibody-drug conjugates (gsADCs), which carry cytotoxic payloads at the conserved -glycosylation site, N297, of an IgG, have emerged as a promising ADC format with better therapeutic index. Conjugating the payloads aldehyde-based chemistry is more friendly to IgGs, and has been widely investigated. However, the efficiency of introducing an aldehyde tag at the N297 site is poor due to the complicated procedures required, such as the multiple-enzyme-catalyzed IgG glycoengineering process and the successive oxidation step, which always results in heterogeneous products and poor stability.

Towards effective functionalization of nanopores/nanochannels: the role of amidation reactions.

In recent years, researchers have drawn inspiration from natural ion channels to develop various artificial nanopores/nanochannels, including solid-state and biological. Through imitating the precise selectivity and single molecule sensing exhibited by natural ion channels, nanopores/nanochannels have been widely used in many fields, such as analyte detection, gene sequencing and so on. In these applications, the surface functionalization of nanopores/nanochannels directly determines the effectiveness in quantitative analysis and single molecule detection.

Tantalum-stabilized ruthenium oxide electrocatalysts for industrial water electrolysis.

The iridium oxide (IrO) catalyst for the oxygen evolution reaction used industrially (in proton exchange membrane water electrolyzers) is scarce and costly. Although ruthenium oxide (RuO) is a promising alternative, its poor stability has hindered practical application. We used well-defined extended surface models to identify that RuO undergoes structure-dependent corrosion that causes Ru dissolution.

Triboelectric Nanogenerator-Based Self-Powered Urinary Protein Detection Utilizing Triboelectric Material with Colorimetric Function.

Chronic kidney disease (CKD) has a high incidence rate, and if not detected and treated in a timely manner, it poses a risk of progressing to renal failure and even uremia. Performing home monitoring of urinary protein, which is a recognized indicator of CKD, is considered an effective means of achieving early warning for CKD. Although the existing urinary protein test strips for home self-testing are cost-effective and simple, they suffer from drawbacks such as susceptibility to contamination and lack of quantitative detection capability.

TarIKGC: A Target Identification Tool Using Semantics-Enhanced Knowledge Graph Completion with Application to CDK2 Inhibitor Discovery.

Target identification is a critical stage in the drug discovery pipeline. Various computational methodologies have been dedicated to enhancing the classification performance of compound-target interactions, yet significant room remains for improving the recommendation performance. To address this challenge, we developed TarIKGC, a tool for target prioritization that leverages semantics enhanced knowledge graph (KG) completion.

Upregulation of RCN2 accelerates tumor progression and indicates poor prognosis in OSCC.

Objective: Oral squamous cell carcinoma (OSCC) is a prevalent cancer of the head and neck region. However, the potential role of RCN2 in OSCC is currently not well understood.

Study Design: A series of molecular biology experiments were conducted to explore the mechanism by which RCN2 promotes OSCC growth through protein kinase A (PKA).

High sound pressure piezoelectric micromachined ultrasonic transducers using sputtered potassium sodium niobate.

This work presents air-coupled piezoelectric micromachined ultrasonic transducers (pMUTs) with high sound pressure level (SPL) under low-driving voltages by utilizing sputtered potassium sodium niobate KNaNbO (KNN) films. A prototype single KNN pMUT has been tested to show a resonant frequency at 106.3 kHz under 4 V with outstanding characteristics: (1) a large vibration amplitude of 3.

Expert consensus on sequential surgery following conversion therapy based on combination of immune checkpoint inhibitors and antiangiogenic targeted drugs for advanced hepatocellular carcinoma (2024 edition).

Up to half of hepatocellular carcinoma (HCC) cases are diagnosed at an advanced stage, for which effective treatment options are lacking, resulting in a poor prognosis. Over the past few years, the combination of immune checkpoint inhibitors and anti-angiogenic targeted therapy has proven highly efficacious in treating advanced HCC, significantly extending patients' survival and providing a potential for sequential curative surgery. After sequential curative hepatectomy or liver transplantation following conversion therapy, patients can receive long-term survival benefits.

Prophylactic ondansetron for preventing intraoperative shivering, nausea and vomiting during spinal anesthesia for cesarean section: a randomized controlled trial.

Background: Shivering, nausea and vomiting are common complications in cesarean section during neuraxial anesthesia. The aim of this study was to investigate the effects of prophylactic use of ondansetron on intraoperative shivering, nausea and vomiting in women undergoing cesarean section.

Methods: A total of 80 full-term parturients were randomly divided into the ondansetron group and the control group.

The Effects of Graded Levels of Calorie Restriction XXI: impact of short term graded restriction on gene expression profiles of stomach and skeletal muscle.

Calorie restriction (CR) extends lifespan and prevents several aging related diseases. During short-term restriction, we previously showed that lean tissues generally decrease in size, but the alimentary tract (especially the stomach) grows. To illuminate pathway alterations in these contrasting tissues we compared gene expression profiles (bulk RNAseq) of the skeletal muscle and stomach, in the same male C57BL/6J mice exposed to 3 months of graded CR (0-40%).

Beta-aminoisobutyric acid improves bovine oocyte maturation and subsequent embryonic development by promoting lipid catabolism.

Energy metabolism homeostasis is essential for oocyte maturation and acquisition of developmental capacity. However, bovine oocyte in vitro maturation (IVM) is highly susceptible to metabolic stress and lipid accumulation. β-Aminoisobutyric acid (BAIBA), a metabolite produced in response to skeletal muscle exercise, has been reported to be involved in lipid and glucose metabolism, as well as inflammation and oxidative stress.

Facile synthesis of high-swelling cyclodextrin polymer for sustainable water purification.

Porous materials are widely used in the adsorption field to sequester pollutants to address the global sustainable water security and water scarcity concerns. However, there are still challenges that limit their industrial application, especially the required rational design and construction of porous structures. Here, we report a high-swelling cyclodextrin polymer (His-CDP) that is facilely synthesized without additional design and templates, to achieve high affinity, non-specific and rapid adsorption of pollutants.

The pitfalls and challenges in the diagnosis and treatment of patients with carotid near-occlusion: a narrative review.

Background And Objective: Carotid near-occlusion (CNO) is defined as a severe stenosis of the internal carotid artery (ICA) resulting in a reduction in the distal diameter. It is a specific type of carotid stenosis accounting for 34% of the patients with symptomatic carotid stenosis ≥50%. The current guidelines recommend the best medical treatment (BMT) as the treatment of choice.

Vascular and Perivascular Role in the Regulation of Angiogenesis: Impact on Arteriovenous Fistula Maturation.

Arteriovenous fistula (AVF) is a surgical connection between an artery and a vein created in patients with end-stage renal disease needing dialysis. A major concern with AVF is maturation failure which results, while creating a new AVF, a troublesome process for the patients. Thus, maturation of AVF is important which is achieved by outflow tract outward remodeling.

Identification and Allergenicity Analysis of Tropomyosin: A Heat-Stable Allergen in .

, a prevalent aquatic delicacy, is known to elicit allergic reactions in certain individuals. Nevertheless, the investigation into its allergenic components has remained notably inadequate. In the research, an approximately 35 kDa heat-stable protein of raw/steamed extracts was verified as tropomyosin (TM) by LC-MS/MS.

Environmental DNA/RNA metabarcoding for noninvasive and comprehensive monitoring and assessment of marine fishes.

Marine fisheries resources are under increasing threat, necessitating the development of new effective monitoring and management strategies. Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has emerged as a non-invasive and sensitive alternative method for monitoring fish biodiversity and fisheries resources and assessing the fisheries impact of anthropogenic activities. Here, we summarize crucial technical details about eDNA metabarcoding for marine fish monitoring and provide meta-analytical trends in primer selection and sample size, assessment standards, fish and fisheries databases, reference fish genomic databases, and other relevant metrics.

Effect of Epidural Volume Extension Using Low-Dose Sufentanil Combined with Low-Concentration Ropivacaine on Visceral Pain During Cesarean Sections: A Randomized Trial.

Background: Visceral pain is common in cesarean sections conducted under combined spinal-epidural anesthesia (CSE). Epidural volume extension (EVE) is a technique for enhancing the effect of intrathecal blocks by inducing epidural fluid boluses in the CSE. Whether EVE that uses different drugs can reduce visceral pain during cesarean sections is rarely studied.

The Built Environment, PTSD Symptoms, and Tobacco Use among Permanent Supportive Housing Residents.

Introduction: 50% of permanent supportive housing (PSH) residents in the U.S. smoke cigarettes, and tobacco-related mortality is their number one cause of death.

Simultaneous 2-photon and 3-photon excitation with a red fluorescent protein-cyanine dye probe pair in the 1700-nm excitation window for deep neurovascular imaging.

imaging of the neurovascular network is considered to be one of the most powerful approaches for understanding brain functionality. Nevertheless, simultaneously imaging the biological neural network and blood vessels in deep brain layers in a non-invasive manner remains to a major challenge due to the lack of appropriate labeling fluorescence probe pairs. Herein, we proposed a 2-photon and 3-photon fluorescence probe pair for neurovascular imaging.

Outer-surface covering of nanochannels with hydrogel for anti-protein-interference on the ion current.

Nanopore/nanochannel sensing is a promising analytical method in the fields of chemistry and biology. However, due to the interference of non-analytes in complex samples, directly analyzing un-pretreated samples through nanopores/nanochannels remains a great challenge. Here, we report a type of heterogeneous membrane by covering anodic aluminum oxide (AAO) cylindrical nanochannel porous membrane with graphene oxide/calcium alginate (GCA) hybrid hydrogel to reduce the interference of protein on the current detection signal.