Publications by authors named "XiRui Chen"

Fluorescent lateral flow immunoassay (LFIA) is recognized as a leading quantitative point-of-care (POC) platform for precise clinical diagnostics. However, conventional fluorescent nanoprobes are hampered by low quantum yield (QY), which constrain the sensitivity of fluorescent LFIA. Herein, we employed a butterfly aggregation-induced emission luminogen (AIEgen) and developed the fully inter-restricted assembly with a polyphenyl polymer poly(maleicanhydride-styrene) (PMPS) to create highly fluorescent homogeneous nanoparticles (ho-AIENPs) with QY over 91%.

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Large language models (LLMs) offer potential in primary dental care. We conducted an evaluation of LLMs' diagnostic capabilities across various oral diseases and contexts. All LLMs showed diagnostic capabilities for temporomandibular joint disorders, periodontal disease, dental caries, and malocclusion.

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Krebs von den Lungen-6 (KL-6) has been recognized as an effective serum biomarker for interstitial lung disease (ILD). The KL-6 accurate detection is of great significance for evaluating the severity of ILD and the prognosis of patients. In this study, a bright aggregation-induced emission luminogen (AIEgen) N, N'-((1,2-diphenylethene-1,2-diyl)bis(4,1-phenylene))bis(N-phenylnaphthalen-1-amine) (TPETN) with a high quantum yield of 87.

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Article Synopsis
  • Repairing large bone defects involves complex processes where different cell types interact to regenerate bone, making it a complex but interesting area of study.
  • Exosomes, tiny vesicles that help cells communicate, play a crucial role in this repair process and have emerged as a promising treatment option due to their effective properties and ability to minimize immune reactions.
  • This study found that exosomes from a specific cell type (Krt14Ctsk) significantly boost the growth and migration of blood vessel cells, enhance bone formation markers, and improve healing in bone defects, indicating their potential for advancing bone repair therapies.
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  • CRISPR diagnostics face challenges like low signal efficiency and sensitivity due to their reliance on single-stranded nucleic acid fluorescent reporters.
  • The new CrisprAIE system combines CRISPR/Cas reactions with innovative aggregation-induced emission luminogens (AIEgens) for more effective fluorescence and improved clinical detection of norovirus and SARS-CoV-2.
  • CrisprAIE shows a substantial increase in sensitivity—up to 270 times better—compared to traditional CRISPR diagnostics and has potential for broader application in improving detection methods across various CRISPR-based techniques.
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Retinal ischemia is an ophthalmic emergency often caused by cardiovascular diseases, leading to irreversible vision loss and even blindness. Innovative retinal ischemia treatments are needed due to limited options. The pathological mechanisms involve retinal cell apoptosis and microglial activation.

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The extensive use of neonicotinoids (NEOs) in agricultural production has led to their pervasive presence in various environmental matrices, including human samples. Given the central role of fruits and vegetables in daily human diets, it is crucial to evaluate the levels of NEOs residues and their potential health risks. In this study, 3104 vegetable samples and 1567 fruit samples from the Shenzhen city were analyzed.

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Wet age-related macular degeneration (wet AMD) is a primary contributor to visual impairment and severe vision loss globally, but the prevailing treatments are often unsatisfactory. The development of conventional treatment strategies has largely been based on the understanding that the angiogenic switch of endothelial cells (ECs) is mainly dictated by angiogenic growth factors. Even though treatments targeting vascular endothelial growth factor (VEGF), like ranibizumab, are widely administered, more than half of patients still exhibit inadequate or null responses, suggesting the involvement of other pathogenic mechanisms.

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Herein, a colorimetric-fluorescent hybrid bifunctional nanobead with Janus structure (J-cf-HBN) was synthesized via one-pot microemulsification. Oleylamine-coated AuNPs and aggregation-induced emission luminogens (AIEgens) were suggested as building blocks to obtain high-performance colorimetric-fluorescent signals. The as-prepared J-cf-HBNs were used as a signal amplification probe to construct an immunochromatographic assay (J-cf-HBNs-ICA) platform for the ultrasensitive detection of staphylococcal enterotoxin B (SEB) in milk samples.

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Choroidal neovascularization (CNV), characterized as a prominent feature of wet age-related macular degeneration (AMD), is a primary contributor to visual impairment and severe vision loss globally, while the prevailing treatments are often unsatisfactory. The development of conventional treatment strategies has largely been based on the understanding that the angiogenic switch of endothelial cells is dictated by angiogenic growth factors alone. Even though treatments targeting vascular endothelial growth factor (VEGF), like Ranibizumab, are widely administered, more than half of the patients still exhibit inadequate or null responses, emphasizing the imperative need for solutions to this problem.

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Aggregation-induced emission luminogen (AIEgen)-functionalized organic-inorganic hybrid nanoparticles (OINPs) are an emerging category of multifunctional nanomaterials with vast potential applications. The spatial arrangement and positioning of AIEgens and inorganic compounds in AIEgen-functionalized OINPs determine the structures, properties, and functionalities of the self-assembled nanomaterials. In this work, a facile and general emulsion self-assembly tactic for synthesizing well-defined AIEgen-functionalized OINPs is proposed by coassembling alkane chain-functionalized inorganic nanoparticles with hydrophobic organic AIEgens.

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Herein we developed a multicolor lateral flow immunoassay (LFIA) test strip for rapid and simultaneous quantitative detection of aflatoxin B1 (AFB1) and zearalenone (ZEN). Three differently colored aggregation-induced emission nanoparticles (AIENPs) were designed as LFIA signal tags, with red and green AIENPs for targeting AFB1 and ZEN at the test line, and yellow AIENPs for indicating the validity of the test strip at the control (C) line. After surface functionalization with antibodies, the developed AIENP-based multicolor LFIA allows simultaneous and accurate quantification of AFB1 and ZEN using an independent C-line assisted ratiometric signal output strategy.

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Article Synopsis
  • * Aggregation-induced emission (AIE) is a promising new concept in luminescence, offering benefits like high brightness, safety for biological use, and stability, making it suitable for medical applications.
  • * This review summarizes the use of AIE luminogens in imaging biological structures, diagnosing diseases, and monitoring specific substances, while also addressing important issues and future research directions to encourage interdisciplinary collaboration.
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Hg contamination in sewage can accumulate in the human body through the food chains and cause health problems. Herein, a novel aggregation-induced emission luminogen (AIEgen)-encapsulated hydrogel probe for ultrasensitive detection of Hg was developed by integrating hydrophobic AIEgens into hydrophilic hydrogels. The working mechanism of the multi-fluorophore AIEgens (TPE-RB) is based on the dark through-bond energy transfer strategy, by which the energy of the dark tetraphenylethene (TPE) derivative is completely transferred to the rhodamine-B derivative (RB), thus resulting in intense photoluminescent intensity.

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Article Synopsis
  • * Researchers introduced aggregation-induced emission luminogens embedded nanospheres (AIENPs) that emit red light and are used as signal amplifiers in LFIA for the detection of zearalenone (ZEN).
  • * The AIENP-LFIA outperformed the traditional time-resolved dye-embedded nanoparticles (TRNPs) by showing stronger photoluminescence and better sensitivity, achieving lower detection limits and improved accuracy for ZEN quantitation in corn samples.
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Staphylococcal enterotoxin A (SEA) has presented enormous difficulties in dairy food safety and the sensitive detection of SEA provides opportunities for effective food safety controls and staphylococcal food poisoning tracebacks. Herein, a novel aggregation-induced emission (AIE)-based sandwich lateral flow immunoassay (LFIA) was introduced to detect SEA by using red-emissive AIE nanoparticles (AIENPs) as the fluorescent nanoprobe. The nanoprobe was constructed by directly immobilising antibodies on boronate-tagged AIENPs (PBA-AIENPs) via a boronate affinity reaction, which exhibited a high SEA-specific affinity and remarkable fluorescent performance.

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Organic fluorescein dye-embedded fluorescent microspheres (FMs) are currently the most established commercially fluorescent markers, and they have been widely used to improve the sensitivity of immunochromatography assay (ICA). However, these FMs have natural defects, such as the aggregation-caused quenching effect and small Stokes shift, which are not conducive to improving the detection performance of ICA. Herein, two green emitted FMs, namely aggregation-induced emission FMs (AIEFMs) and fluorescein isothiocyanate FMs (FITCFMs), were prepared by swelling the AIE luminogens and FITC dyes into the carboxyl group-modified polystyrene microspheres.

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Herein, we report a novel aggregation-induced emission nanoparticles (AIENPs)-based immunochromatography assay (ICA) platform to detect ochratoxin A (OTA) using orange-yellow-emitting AIENPs as fluorescent nanoprobes. Immunochromatographic strip is used for the quantitative detection of OTA in crop matrix using AIENPs coupled with anti-OTA ascites. Under optimal conditions, AIENPs-ICA exhibits stronger signal output capacity and higher sensitivity than traditional gold nanoparticles-based ICA.

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Lateral flow immunoassay (LFIA) based on fluorescent microbeads has attracted much attention for its use in rapid and accurate food safety monitoring. However, conventional fluorescent microbeads are limited by the aggregation-caused quenching effect of the loaded fluorophores, thus resulting in low signal intensity and insufficient sensitivity of fluorescent LFIA. In this study, a green-emitting fluorophore with an aggregation-induced emission (AIE) characteristic was encapsulated in polymer nanoparticles via an emulsification technique to form ultrabright fluorescent microbeads (denoted as AIEMBs).

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Ultrabright green-emissive AIE nanoparticles (AIENPs) were used as signal-amplification probes to enhance the detectability of lateral flow immunoassay (LFIA). The detection performances of the green-emissive AIENP probes in both sandwich and competitive LFIA formats were systematically evaluated. Benefiting from its remarkable fluorescent brightness, the developed AIENP-LFIA showed versatile applicability for the detection of small molecules and macromolecules by using ochratoxin A (OTA) and procalcitonin (PCT) as model analytes, respectively.

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Staphylococcal enterotoxin A (SEA) is an important biotoxin, produced by Staphylococcus aureus under appropriate conditions, and often contaminates milk and dairy products. Herein, an anti-SEA monoclonal antibody (anti-SEA mAb) was prepared by injecting the SEA protein in BALB/c mice, and a novel immunochromatographic assay (ICA) was developed for the rapid and sensitive determination of SEA in pasteurized milk by using highly luminescent quantum dot beads (QB) as signal amplification probe. Given the 1020-fold enhancement in the photoluminescence intensity of QB to the original quantum dot, the proposed QB-ICA exhibits high sensitivity for SEA determination in real milk samples with a limit of detection of 1.

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Herein, we developed a paper-based smart sensing chip for the real-time, visual, and non-destructive monitoring of food freshness using a ratiometric aggregation-induced emission (AIE) luminogen (i.e., HMQ, protonated 4-(triphenylamine)styryl)quinoxalin-2(1)-one) as pH sensitive indicators.

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Gold nanoparticle-based immunochromatographic assay (AuNP-ICA) has insufficient sensitivity due to its inherent colorimetric signal intensity and low capture efficiency of AuNPs. The metal growth is a common strategy to enhance the sensitivity of AuNP-ICA due to its superior signal amplification potential and simple operation. However, the detection distortion caused by metal self-nucleation during the growth process can seriously affect the accuracy and reproducibility of the strips.

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Background: The elderly is a vulnerable group susceptible to toxic environmental factors; however, the association between unsafe water and osteoarthritis (OA) incident among this population is poorly investigated. This study aimed to examine the effects of drinking water sources on OA risk in the Chinese elderly population.

Methods: Data were obtained from the China Longitudinal Healthy Longevity Survey to perform a 16-year longitudinal study.

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Article Synopsis
  • The traditional gold nanoparticles (AuNPs) used in colorimetric lateral flow immunoassays (LFIA) are often limited in sensitivity due to their insufficient signal brightness.
  • A new "three-in-one" multifunctional nanohybrid (FeO@MOF@Pt) has been developed, combining FeO nanoparticles, MIL-100(Fe), and platinum nanoparticles, which enhances colorimetric signal brightness and sensitivity.
  • This new system can achieve a sensitivity of 0.5 pg/mL for procalcitonin testing, which is about 2280 times greater than conventional AuNP-based LFIA, indicating its potential for improved disease diagnostics.
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