Humanized cerebral organoids-based ischemic stroke model for discovering of potential anti-stroke agents.

Establishing a stoke experimental model, which is better in line with the physiology and function of human brain, is the bottleneck for the development of effective anti-stroke drugs. A three-dimensional cerebral organoids (COs) from human pluripotent stem cells can mimic cell composition, cortical structure, brain neural connectivity and epigenetic genomics of in-vivo human brain, which provides a promising application in establishing humanized ischemic stroke model. COs have been used for modeling low oxygen condition-induced hypoxic injury, but there is no report on the changes of COs in response to in vitro oxygen-glucose deprivation (OGD)-induced damage of ischemic stroke as well as its application in testing anti-stroke drugs.

Determination of cyflufenamid residues in 12 foodstuffs by QuEChERS-HPLC-MS/MS.

Agricultural products are often contaminated with trace amounts of pesticide residues. To ensure food safety, a reliable, sensitive, and efficient method that accurately identifies pesticide residues in a wide variety of foodstuffs is needed. This study applied a modified QuEChERS extraction technique, coupled with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to optimize the identification of the cyflufenamid, a new broad-spectrum fungicide with unclear mode of action in toxicity, in 12 foodstuffs.

Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium.

Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment.

Liquid Crystals in Curved Confined Geometries: Microfluidics Bring New Capabilities for Photonic Applications and Beyond.

The quest for interesting properties and phenomena in liquid crystals toward their employment in nondisplay application is an intense and vibrant endeavor. Remarkable progress has recently been achieved with regard to liquid crystals in curved confined geometries, typically represented as enclosed spherical geometries and cylindrical geometries with an infinitely extended axial-symmetrical space. Liquid-crystal emulsion droplets and fibers are intriguing examples from these fields and have attracted considerable attention.

Combined use of Y-tube conduits with human umbilical cord stem cells for repairing nerve bifurcation defects.

Given the anatomic complexity at the bifurcation point of a nerve trunk, enforced suturing between stumps can lead to misdirection of nerve axons, thereby resulting in adverse consequences. We assumed that Y-tube conduits injected with human umbilical cord stem cells could be an effective method to solve such problems, but studies focused on the best type of Y-tube conduit remain controversial. Therefore, the present study evaluated the applicability and efficacy of various types of Y-tube conduits containing human umbilical cord stem cells for treating rat femoral nerve defects on their bifurcation points.

[Effect of wortmannin on endothelial cell proliferation and migration induced by high glucose Müller cell conditioned medium].

Objective: To investigate the effect of wortmannin on endothelial cells proliferation and migration induced by high glucose Müller cell conditioned medium (HGMCM).

Methods: Immunofluorescence, flow cytometry and Boyden chamber migration models were used to analyze the effect of wortmannin on endothelial cells.

Results: 50 nmol/L wortmannin could significantly inhibit the proliferation and migration of endothelial cells induced by HGMCM.