[Efficacy of prolonged azithromycin versus switching to doxycycline in the treatment of macrolide-unresponsive pneumonia in children].

Objectives: To investigate the efficacy and safety of prolonged azithromycin (PAZM) versus switching to doxycycline (SDXC) in the treatment of macrolide-unresponsive pneumonia (MUMPP) in children.

Methods: A total of 173 children with MUMPP who were hospitalized in Baoji Central Hospital, from January to December 2023 were selected as subjects. According to the choice of secondary antibiotic after 72 hours of initial macrolide therapy, they were divided into two groups: PAZM and SDXC.

Primary repair of esophageal atresia gross type C thoracoscopic magnetic compression anastomosis: A case report.

Background: Esophageal atresia (EA) is a life-threatening congenital malformation in newborns, and the traditional repair approaches pose technical challenges and are extremely invasive. Therefore, surgeons have been actively investigating new minimally invasive techniques to address this issue. Magnetic compression anastomosis has been reported in several studies for its potential in repairing EA.

Neonatal isovaleric acidemia in China: A case report and review of literature.

Background: Isovaleric acidemia (IVA) is a rare autosomal recessive inherited organic acidemia caused by a genetic deficiency of isovaleryl-CoA dehydrogenase (IVD). Its morbidity is low, but mortality is high. There is no effective cure for this disease.

[A comparative analysis of the efficacy of two vitamin D supplementation regimens in preterm infants: a prospective randomized controlled study].

Objective: To study the effect of different vitamin D supplementation regimens on the nutritional status of vitamin D on day 28 after birth in preterm infants with a gestational age of <34 weeks.

Methods: A total of 59 preterm infants with a gestational age of <34 weeks who were born from October 2018 to October 2019 were enrolled and divided into an observation group with 30 infants and a control group with 29 infants. The infants in the observation group received a single-dose intramuscular injection of vitamin D (10 000 IU/kg), while those in the control group received oral vitamin D drops (900 IU/d) for 25 days.

[Sedative effect of intranasal midazolam in neonates undergoing magnetic resonance imaging: a prospective single-blind randomized controlled study].

Objective: To compare intranasal midazolam and intramuscular phenobarbital sodium for their sedative effect in neonates undergoing magnetic resonance imaging (MRI).

Methods: A total of 70 neonates who underwent cranial MRI from September 2017 to March 2019 were randomized into an observation group and a control group, with 35 cases in each group. The observation group received intranasal drops of midazolam (0.

[The Function of Potassium Channel in G271V Mutants of Benign Familial Neonatal Convulsions].

Objective: To determine the function of potassium channel in G271V mutants of benign familial neonatal convulsions.

Methods: HEK293 cells were transfected with pcDNA3-WT- and / or pcDNA3-G271V- and pcDNA3-WT- The potassium channel function of G271V mutants was assessed using the whole cell patch clamp technique.

Results: G271V mutants did not show currents in the transfected HEK cells, inducing large depolarizing shift of the conductance voltage relationship and slowing down the current activation kinetics.

[Influence of acute pancreatitis in pregnancy on pregnancy outcomes and neonates].

Objective: To study the influence of acute pancreatitis in pregnancy (APIP) on pregnancy outcomes and neonates.

Methods: A retrospective analysis was performed for 33 APIP patients and 31 neonates born alive.

Results: Of the 33 APIP patients, 26 (79%) developed APIP in the late pregnancy.

[Site-directed mutagenesis and protein expression of ABCA3 gene in A549 cells].

Objective: To study the protocol of construction of the mutation E292V and M723I of hABCA3 gene associated with neonatal respiratory distress syndrome, as well as their eukaryotic green fluorescent protein expression rectors, and to examine the expression of mutation proteins in human lung carcinoma epithelial cells (A549).

Methods: Site-directed mutagenesis method based on overlap extension PCR was used to introduce mutations in the two sites which were E292V and M723I in the ABCA3. The PCR fragments were subcloned to PEGFP-C2 vectors to construct the eukaryotic green fluorescent protein expression rectors.

Semi-quantitative assessment of brain maturation by conventional magnetic resonance imaging in neonates with clinically mild hypoxic-ischemic encephalopathy.

Background: Mild hypoxic-ischemic encephalopathy (HIE) injury is becoming the major type in neonatal brain diseases. The aim of this study was to assess brain maturation in mild HIE neonatal brains using total maturation score (TMS) based on conventional magnetic resonance imaging (MRI).

Methods: Totally, 45 neonates with clinically mild HIE and 45 matched control neonates were enrolled.

Hyperbaric oxygenation reduces long-term brain injury and ameliorates behavioral function by suppression of apoptosis in a rat model of neonatal hypoxia-ischemia.

Neonatal hypoxia-ischemia (HI) produces neurodegeneration and brain injury, and leads to behavioral and cognitive dysfunction. Hyperbaric oxygen (HBO) treatment may potentially be neuroprotective in HI injury. The aim of this study was to examine any neuroprotection by HBO treatment on long-term neurological function in the rat model of neontatal HI.

Clinical and pathological features of a neonate with autosomal recessive polycystic kidney disease caused by a nonsense PKHD1 mutation.

Background: Autosomal recessive polycystic kidney disease (ARPKD) is one of the most common hereditary nephropathies in childhood. We report a neonate with ARPKD presenting with oligohydramnios, enlargement and increased echogenicity of both kidneys shown by antenatal sonograms after a 29-week gestation and died within the first few hours of life.

Methods: The neonate was investigated pathologically post-mortem.

Site-directed mutagenesis of neonatal convulsions associated KCNQ2 gene and its protein expression.

Objective: To study the protocol of construction of a KCNQ2-c.812G>T mutant and its eukaryotic expression vector, the c.812G>T (p.

[Detection of genetic defect within ABCA3 from newborns with respiratory distress syndrome].

Objective: To detect possible relationship between genetic defect within the gene encoding member A3 of the ATP Binding Cassette family (ABCA3) and neonatal respiratory distress syndrome (NRDS), thus to understand the genetic mechanisms of NRDS in Han ethnic group.

Method: The clinical data of 11 cases with NRDS hospitalized in neonatal intensive care unit was investigated. Blood samples were collected from 11 cases with NRDS and 97 unassociated normal individuals.

[Site-directed mutagenesis and protein expression of KCNQ2 gene associated with neonatal convulsions].

Objective: To study the protocol of construction of a KCNQ2-c.812G>T mutant and it's eukaryotic expression vector, the c.812G>T (p.

[Effect of inhibitors of phospholipase A(2); on the metastasis potentials of human ovarian cancer cells].

Aim: it is important to find inhibitors for metastasis of ovarian cancer (OC). OC cells (OCC) produce Lysophosphophatidic acid (LPA), which promotes the OC development. Phospholipase A(2); (PLA(2);) is responsible for LPA production.

[Linkage analysis and gene mapping of one Chinese family with benign familial infantile convulsions].

Objective: The present study performed linkage analysis and gene mapping to find the possible chromosome locus harboring in one family with benign familial infantile convulsions (BFIC) and investigate the possible molecular pathogenesis of BFIC.

Methods: A four-generation family with BFIC was investigated. The family was genotyped using eight hypervariable microsatellite markers covering four loci: D19S245 and D19S250 for the 19q12-13.

[Gene mutation analysis of a Chinese family of congenital long Q-T syndrome type three].

Objective: The congenital long QT syndrome (LQTs) is a hereditary disorder in which most affected family members have delayed ventricular repolarization manifested on the electrocardiogram (ECG) as QT interval prolongation. The disorder is associated with an increased propensity to arrhythmogenic syncope, polymorphous ventricular tachycardia (torsade de pointes), and sudden arrhythmic death. LQTs is due to mutations involving principally the myocyte ion-channels, and this monogenetic disorder has an autosomal inheritance pattern.

[Hyperbaric oxygen improves long-term learning-memory deficits and brain injury in neonatal rat with hypoxia-ischemia brain damage].

Objective: To investigate the effect of hyperbaric oxygen (HBO) on long-term learning-memory disabilities and brain injury induced by hypoxia-ischemia in neonatal rat.

Methods: In the study, eighteen rats aged seven days were divid into three groups: (1) sham-operated group (SHAM), (2) hypoxia-ischemia group (HIBD), (3) HBO-treated hypoxia-ischemia group (HIBD + HBO). In hypoxia-ischemia groups, left common carotid artery was ligated permanently on the seventh postnatal day, two hours after the procedure; hypoxia (92% nitrogen and 8% oxygen) was induced for 2 h.

[A novel mutation in KCNQ2 gene causes benign familial infantile convulsions (BFIC) in a Chinese family].

Objective: Benign familial infantile convulsions (BFIC) is a form of idiopathic epileptic syndrome characterized by onset of afebrile seizures between 3 and 12 months of life, Spontaneous remission after several weeks or months, and autosomal dominant mode of inheritance. Previous linkage analysis in western countries defined three susceptible loci on chromosomes 19q12.0-13.

[Optimal therapeutic window of hyperbaric oxygenation in neonatal rat with hypoxic-ischemic brain damage].

Objective: Hyperbaric oxygenation (HBO) is an attractive procedure that has been used in treatment of hypoxic-ischemic encephalopathy (HIE). However, depending on the HBO protocol, especially the time point of starting treatment of HBO, different and conflicting results were obtained. This study was undertaken to search for the optimal therapeutic window of ABO in neonatal rat with hypoxic-ischemic brain damage (HIBD).