UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.

To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated. Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively.

Functions of a Glucan Synthase Gene in Mycelial Growth and Polysaccharide Production of .

Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene () in the edible mushroom with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp is a membrane protein containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain.

[The progress and prospect of prepulse inhibition in autism].

Prepulse inhibition (PPI) is suppression of the startle reflex when an intense startling stimulus is preceded by a weaker sensory stimulus (the prepulse). It is an operational measurement of sensorimotor gating mechanism to help human adapt to complex environment. This weak prepulse protect central cognitive processing by damping the effect of intense stimuli.

Interaction between auditory and motor systems in speech perception.

Based on the Motor Theory of speech perception, the interaction between the auditory and motor systems plays an essential role in speech perception. Since the Motor Theory was proposed, it has received remarkable attention in the field. However, each of the three hypotheses of the theory still needs further verification.

[Behavioral and neurobiological abnormalities induced by social isolation as a useful animal model of schizophrenia].

Social isolation influences the development of the brain, causing dysfunctions at behavioral, cellular and molecular levels. The present paper summarizes the abnormalities induced by social isolation in behaviors, neurotransmitters and cell apoptosis. At the behavioral level, social isolation induces hyperlocomotion, abnormalities in startle reflex and prepulse inhibition (PPI), and dysfunctions in conditioned learning, reversal learning and memory.

The dual-pathway model of auditory signal processing.

Similar to the visual dual-pathway model, neurophysiological studies in non-human primates have suggested that the dual-pathway model is also applicable for explaining auditory cortical processing, including the ventral "what" pathway for object identification and the dorsal "where" pathway for spatial localization. This review summarizes evidence from human neuroimaging studies supporting the dual-pathway model for auditory cortical processing in humans.

[Speciation of 9 trace elements in a traditional Chinese medicine long dan cao by flow injection-inductively coupled plasma mass spectrometry with a focused microwave assisted extraction system].

A method based on focused microwave extraction for leaching 9 trace elements in traditional Chinese medicine Long Dan Cao was introduced. An online C18 enrichment-separation system and an HP4500 ICP mass spectrometer with a flow injection system was established for the separation and determination of inorganic speciation and organic speciation of the selected elements. Orthogonal design was applied for the optimization of microwave leaching conditions.