[Preparation and identification of the rabbit antibody against human sialin].

Aim: To prepare the rabbit antibody against human sialin and identify its properties.

Methods: Recombinant expression vector pGEX-5X-1-sialin was constructed, in which the sialin cDNA encoding the 1-38 aa was fused to the C-terminal of the gene encoding the GST protein. The GST-sialin (N1-38) fusion protein was expressed in E.