Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells.

Background: A decrease in the number and activity of thymic epithelial cells (TECs) is an important factor in thymic degeneration. Mesenchymal stem cells (MSCs) treating thymic ageing is a promising strategy, but the DNA methylation modification mechanism in TECs remains unclear.

Methods: Aged rhesus monkeys were treated with MSCs to establish a thymic senescence model, and hematoxylin-eosin (HE) staining, immunofluorescence staining, and ELISA were performed to observe the structure and function of the thymus.

[Occurrence Characteristics, Sources, and Toxicity Risk Analysis of Polycyclic Aromatic Hydrocarbons in Typical Rivers of Northern Shaanxi Mining Area, China].

The aim of this study was to assess the occurrence level, spatial distribution, pollution source, and ecological risk of polycyclic aromatic hydrocarbons (PAHs) in the Kuye River of the northern Shaanxi mining area. In total, 16 priority PAHs were quantitatively detected at 59 sampling sites using a high-performance liquid chromatography-diode array detector in series with a fluorescence detector. The results showed that the (ΣPAHs) in the Kuye River ranged from 50.

Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9.

A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated.

[Clinical effect of manipulation therapy for the treatment of degenerative lumbar instability based on myofascial chain theory].

Objective: To explore clinical effects of manipulation therapy in treating degenerative lumbar instability based on myofascial chain theory.

Methods: Fifty-seven patients with degenerative lumbar spine instability were analyzed retrospectively from January 2018 to December 2019, and treated with massage manipulation therapy. Among them, 29 patients were treated with massage manipulation therapy based on the myofascial chain theory (myofascial chain group), including 14 males and 15 females, aged from 40 to 69 years old with an average of (51.

A simple and quick method to detect adulterated sesame oil using 3D fluorescence spectra.

Adulterated sesame oil seriously damages the interests of consumers and the health of market. In this paper, a simple, fast and real-time model for identifying adulterated sesame oil (ASO) was proposed by combining 3D fluorescence spectra with wavelet moments (WMs). First, noise and data volume of the experimental data were reduced by wavelet multiresolution decomposition (WMRSD), which improved the stability and real-time of the model.

[Molecular Mechanisms of Apoptosis of Leukemia Cell Induced by Reovirus].

Objective: To investigate the molecular mechanism of apoptosis of HL60 cells induced by oncolytic virus Reovirus type 3 (Reo3).

Methods: HL60 cells were infected with Reo3 at different multiplicity of infection (MOI) with the uninfected HL60 cells as control group. After 48 h of infection, the activity of HL60 cells infected with virus at different MOI was detected by CCK8 method to investigate the influence of MOI to cell activity.

[Application of Fluorescence Spectra and Matrix Analysis in the Classification of Edible Vegetable Oils].

The fluorescence spectra of 42 vegetable oil samples are measured with FS920 fluorescence spectrometer, including 36 pure vegetable oil samples, 3 blend oil samples and 3 mixed oil samples. The fluorescence matrixs (EEMs) are normalized to determine representative excitation wavelength and matrix analysis model of vegetable oils. Vegetable oils are divided into three categories by analyzing representative emission spectra and contour maps in the range of emission wavelength from 260 to 750 nm, and excitation wavelengths from 250 to 550 nm.

Withaferin A activates stress signalling proteins in high risk acute lymphoblastic leukemia.

Withaferin A, the principal bio-active component isolated from the Withaniasomnifera, has shown promising anti-leukemic activity in addition to anti-invasive and anti-metastatic activity. The present study demonstrates the effect of withaferin A on the cell cycle status and the phosphorylation/activation of proteins involved in signal transduction in t(4;11) and non-t(4;11) acute lymphoblastic leukemia (ALL) cell lines after treatment with withaferin A. The cells after treatment with the vehicle or 25 μM withaferin A for 1, 2, 4 and 8 h were examined using flow cytometric analysis.

[Application of fluorescence spectra and parallel factor analysis in the classification of edible vegetable oils].

The fluorescence spectra of 22 samples of 8 kinds of edible vegetable oils (soybean oil, maize oil, olive oil, rice oil, peanut oil, walnut oil, sunflower oil and sesame oil) were measured with FS920 fluorescence spectrometer and the fluorescence matrixs (EEMs) were analyzed with parallel factor (PARAFAC) analysis model. To synthesize the capabilities of material characterization and component identification, fluorescence spectra combined with PARAFAC fulfill the classification of vegetable oils. The map feature (peak position, peak value and peak number) was obtained by analyzing three dimensional spectra and con tour maps in the range of emission wavelength from 260 to 750 nm, and excitation wavelengths from 250 to 550 nm.

[Study on phase-matching of four-wave mixing spectrum in photonic crystal fiber].

In the present paper, the four-wave mixing principle of fiber was analyzed, and the high-gain phase-matching conditions were shown. The nonlinear coefficient and dispersion characteristics of photonic crystal fibers were calculated by multipole method. The phase mismatch characteristics of fibers with multiple zero-dispersion wavelengths were analyzed for the first time.

[Study on water quality monitoring scheme based on non-point source pollution].

In order to improve standardization and normalization of non-point source pollution monitoring, this paper summarized the non-point source pollution monitoring scheme that based on conventional technology condition. The scheme firstly emphasized the preparation work before monitoring, including situation investigation and index selection of the monitoring area and so on; In the process of establishing monitoring scheme, the monitoring area was divided into three types: city, agriculture and watershed. Take urban area monitoring scheme for Xi'an as an example, through dividing function zone setting sampling point, summarized sampling time interval, frequency and sampling methods during a rainfall process.

[Application of PARAFAC method and 3-D fluorescence spectra in petroleum pollutant measurement and analysis].

A method for identification and concentration measurement of petroleum pollutant by combining three-dimensional (3-D) fluorescence spectra with parallel factor analysis (PARAFAC) was proposed. The main emphasis of research was the measurement of coexisting different kinds of petroleum. The CCl4 solutions of a 0# diesel sample, a 97# gasoline sample, and a kerosene sample were used as measurement objects.

[Research on fiber methane sensing system based on prism gas cell].

A novel fiber methane detection system was constructed based on integration of prism gas cell and harmonic detection technique. The system can be applied to broad-range concentration detection. Grounded on the Beer-Lambert approximation, the detection of various concentration (0-20%) of methane was completed using subtraction of background and ratio processing method, as the atmosphere surroundings was treated as background.