Dinoflagellates are important primary producers and major causative agents of harmful algal blooms in the global ocean. Despite the great ecological significance, the photosynthetic carbon acquisition by dinoflagellates is still poorly understood. The pathways of photosynthetic carbon assimilation in a marine dinoflagellate Prorocentrum donghaiense under both in situ and laboratory-simulated bloom conditions were investigated using a combination of metaproteomics, qPCR, stable carbon isotope and targeted metabolomics approaches.
View Article and Find Full Text PDFA total of 14 samples were collected in May 2016(dry season)and August 2016 (wet season) in the downstream area of the Taizi River. △ N-NO and △ O-NO were determined using the azide method, and △ O-HO was determined using a CO-HO equilibration technique. To identify NO sources and transformations in the downstream area of Taizi River Basin, ion chromatography, Nessler's reagent spectrophotometry, the azide method, and CO-HO equilibration methods were utilized to determine the concentrations of NO, Cl, NH-N, and isotopic compositions (△ N and △ O) of NO and the △ O-HO in surface water.
View Article and Find Full Text PDFAlthough deemed important to δ O measurement by on-line high-temperature conversion techniques, how the GC conditions affect δ O measurement is rarely examined adequately. We therefore directly injected different volumes of CO or CO-N mix onto the GC column by a six-port valve and examined the CO yield, CO peak shape, CO-N separation, and δ O value under different GC temperatures and carrier gas flow rates. The results show the CO peak area decreases when the carrier gas flow rate increases.
View Article and Find Full Text PDFDistribution of methane in surface water and sediment of Jiulongjiang Estuary was investigated during July, 2009 through head-space method. The concentration of methane varies from 10.7 to 456.
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