Publications by authors named "Xi-Hua Guo"

Article Synopsis
  • * Key findings revealed that Cu, Al, and Mn were absent in rhizoma dioscoreae, while Mg, Zn, and Cr were present; starches showed different crystal types between the two species.
  • * The combination of XRF and PXRD proved effective for analyzing elemental and micro-structural differences in Dioscorea opposita Thunb from various regions.
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The major components of the 4th generation outer space bred angelica and the ground group were determined and analyzed by Fourier transform infrared spectroscopy (FTIR) and second derivative spectrum, considering the large mutation of the plants with space mutagenesis. The results show that the content of the coumarin (1741 cm(-1)), which is the main active components of the space angelica dahurica increased, and the content of the protein (1 459, 1 419 cm(-1)) and the fat (930 cm(-1)) increased slightly, whereas the content of the starch and the dietary fiber reduced drastically. There are obvious differences between the peak values of the second derivative spectra of the plants, revealing that the outer space angelica dahurica contained amine component at 1 279 cm(-1).

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The contents of various elements in the fourth generation atractylodes macrocephala koidz cultivated by spaceflight breeding were analyzed and compared with those of the ground group on the whole jointly by XRF and PXRD. The results showed that the element Na and Ti could not be detected in the space group, and the levels of K and Mn were enhanced by 1.16 and 1.

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X-ray fluorescence spectrum (XRF) analytic method was applied to the determination of the contents and varieties of mineral elements in the space flight mutagenesis breeding scutellaria baicalensis Georgi, and compared with the ground group which were planted and collected together under the same conditions, in an attempt to search for the influence of space environment upon mutation on scutellaria baicalensis Georgi. The result indicates that the varieties of the main mineral elements in the two samples are samely basically, therefore, the two samples have same absorption and enrichment capability of specific element, but the contents of elements Ca, Na, Zn and S increase by 0.3, 0.

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Studied with X-ray fluorescence spectrum analytic (XRF), powder X-ray diffraction (PXRD) and Fourier transform infrared spectroscopy (FTIR) for five different species of Salvia miltiorrhiza bge grew in Anguo, the intrinsic components and microstructures were determined and comprehensive characterization was carried out. The figure of comprehensive characterization can describe characteristics of intrinsic components from elements, crystal and amorphous active component, it can give an intuitive description for the same components and subtle differences in the intrinsic components of Salvia miltiorrhiza bge, which are different idioplasmatic and same species. It is the ideal method because of the simple preparation of sample, rapid and fine accurate measurement with reproducibility and objectivity, so that it can be used for breeding of fine varieties of Chinese medicinal materials, Chinese medicinal materials GAP guidance of growing conditions, medicinal origin characteristics, the intrinsic composition and microstructure characterization, quality control and authenticity of the identification.

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Article Synopsis
  • The study utilized X-ray fluorescence spectrum (XRF) analysis to compare mineral elements in ground and space flight mutagenized Salvia miltiorrhiza Bge.
  • Results showed similar main mineral element varieties, but an increased Cu/Zn ratio and higher levels of microelements (Cr, Mn, and Fe) in the space group.
  • XRF is advantageous for quickly and sensitively analyzing elements in Chinese herbal medicines, providing valuable data for selecting quality breeds.
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FTIR spectra of fourth-generation space bred radix isatidis and FTIR spectra of radix isatidis in a ground group were determined on the whole. Comparative analysis was conducted on main constituents (components) to accumulate data for comprehensively understanding and thoroughly studying the inherent property, and to explore a method of evaluating the quality of space bred radix isatidis. The results showed that slight difference existed in the positions and shapes of the absorption peaks in FTIR spectra of samples between the two groups.

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Determination and analysis for the Scutellaria baicalensis Georgi with space mutagenesis and the ground group were carried out with FTIR for the first time in order to fully understand the quality changes of the 4th generation of space mutagenesis of Scutellaria baicalensis Georgi and do further research. The result shows that for the FTIR spectra of the two samples the position and shape of main absorption peaks are approximately the same, indicating that the major components and the structures of space group remain intact, but the intensities of the absorption peaks are obviously different, with the intensities of the space group significantly enhanced compared to the ground group, especially for the flavonoid compounds (baicalin, baicalein, wogonoside, wogonin and wogonoside etc), which are the main active components of the Scutellaria baicalensis Georgi, the absorption peak intensities at 3391, 1655 and 1069 cm(-1) are obviously stronger than those of the ground group, so the contents of flavonoid compounds and other components are higher, and the amorphous active components are optimized. Space mutation breeding is conducive to breeding new varieties of highly active components, and it is also one of the ways to innovate Chinese medicines germplasm resources efficiently.

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Objective: To study the serum level of interleukin-17A (IL-17A) and its expressions in the lung, spleen and thymus in asthmatic mice.

Methods: In 14 normal BALB/c female mice and 14 asthmatic mice, the changes in the airway pathology and the cell proportion in the bronchoalveolar lavage fluid (BALF) were observed. The serum level of IL-17A and IL-17A expressions in the tissue homogenates of the lung, spleen and thymus of the mice were detected by sandwich enzyme-linked immunosorbent assay (ELISA).

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Objective: To establish a method for analyzing amorphous organic compound components of space Platycodon grandflorum rapidly.

Method: FTIR was applied for measuring and comparing P. grandflorum of comparison group,the ground group and the 4" generation of space group.

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Article Synopsis
  • - The study compared outer space Saposhnikovia divaricata of the fourth generation with ground and comparison groups for the first time, revealing stronger carbonyl absorption indicating enhanced chromone content.
  • - Peaks indicating --CH2-- groups and C-O groups showed higher intensity in the outer space samples, suggesting increased polysaccharide and glycoside content.
  • - The lactone peak intensity indicated that coumarin content was higher than the ground group but lower than the comparison, while major components remained intact, confirming the effective component enhancement in the outer space samples.
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To cultivate oriented new varieties, Saposhnikovia divaricata seeds were carried by Shenzhou "3" satellite and filtered when they were being brought back. In the present paper, X-ray fluorescence(XRF) was used to mensurate and analyze the contents of elements of the two samples from the ground group and the 4th generation of space Saposhnikovia divaricata (space group) that have advantages in branches and leaves and yields. The contents of the main mineral elements in the two samples are the same basically.

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The contents of various elements in the fourth generation Belamcanda chinensis (L.) DC. with space mutagenesis breeding were analyzed and characterized.

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