[Cloning and expression of cucumber CsEXP10 gene].

Basing on the fragment (CO434610) cloned from cDNA amplified fragment length polymorphism (cDNA-AFLP), a 1191-bp cDNA sequence of CsEXP10 with a complete 3' end was obtained via rapid amplification of cDNA ends (RACE) and getting spliced with an EST (AF319471) of the same gene (Fig.2). Southern blotting analysis showed that CsEXP10 was a single copy gene in cucumber (Fig.