Fluorous hydrogenation.

The application of fluorous phosphine-modified catalysts for the hydrogenation of olefins is reviewed.

[Effect of penetrative needling of scalp-acupoints on cerebral N-acetyl-aspartate and choline levels in intracerebral hemorrhage rabbits].

Objective: To observe the effect of scalp-acupuncture on N-acetyl-aspartate (NAA) and choline (Cho) leveIs in the brain tissue in rabbits with intracerebral hemorrhage (ICH) so as to explore its underlying mechanisms in resisting neural lesion.

Methods: A total of 30 Newzealand rabbits were randomly divided into normal, model and acupuncture groups (n=10). ICH model was duplicated by injection of the rabbit-own blood (200 microL) into the bilateral Basal Nuclei (A: 2 mm; R, L; 5 mm; H: 10 mm).

Concomitant forms of abuse and help-seeking behavior among white, African American, and Latina women who experience intimate partner violence.

This study uses National Violence against Women Survey data to investigate the differential impact of concomitant forms of violence (sexual abuse, stalking, and psychological abuse) and ethnicity on help-seeking behaviors of women physically abused by an intimate partner (n = 1,756). Controlling for severity of the physical abuse, women who experienced concomitant sexual abuse are less likely to seek help, women who experienced concomitant stalking are more likely to seek help, whereas concomitant psychological abuse is not associated with help seeking. Ethnic differences are found in help seeking from friends, mental health professionals, police, and orders of protection.

Trichinella spiralis paramyosin binds to C8 and C9 and protects the tissue-dwelling nematode from being attacked by host complement.

Background: Paramyosin is a thick myofibrillar protein found exclusively in invertebrates. Evidence suggested that paramyosin from helminths serves not only as a structural protein but also as an immunomodulatory agent. We previously reported that recombinant Trichinella spiralis paramyosin (Ts-Pmy) elicited a partial protective immunity in mice.

Accurate landmarking of three-dimensional facial data in the presence of facial expressions and occlusions using a three-dimensional statistical facial feature model.

Three-dimensional face landmarking aims at automatically localizing facial landmarks and has a wide range of applications (e.g., face recognition, face tracking, and facial expression analysis).

[Aflatoxin contamination of corn samples collected from six regions of China].

Objective: To identify the aflatoxins contamination of corn in China.

Methods: Aflatoxins B1, B2, G1 and G2 in 279 corn samples collected from 6 provinces or autonomous regions were determined by HPLC.

Results: Aflatoxins were detected in 75.

Combining gene signatures improves prediction of breast cancer survival.

Background: Several gene sets for prediction of breast cancer survival have been derived from whole-genome mRNA expression profiles. Here, we develop a statistical framework to explore whether combination of the information from such sets may improve prediction of recurrence and breast cancer specific death in early-stage breast cancers. Microarray data from two clinically similar cohorts of breast cancer patients are used as training (n = 123) and test set (n = 81), respectively.

Drosophila long-chain acyl-CoA synthetase acts like a gap gene in embryonic segmentation.

Long-chain acyl-CoA synthetases (ACSLs) convert the long chain fatty acids to acyl-CoA esters, the activated forms participating in diverse metabolic and signaling pathways. dAcsl is the Drosophila homolog of human ACSL4 and their functions are highly conserved in the processes ranging from lipid metabolism to the establishment of visual wiring. In this study, we demonstrate that both maternal and zygotic dAcsl are required for embryonic segmentation.

[Pharmacodynamics, pharmacokinetics and tissue distribution of liposomal mitoxantrone hydrochloride].

This study is to compare the pharmacodynamics, pharmacokinetics and tissue distribution of liposomal mitoxantrone (Mit-lipo) and free mitoxantrone (Mit-free). The antineoplastic effect of Mit-lipo was evaluated on PC-3 human xenograft tumor model after repeated intravenous injection at dose levels of 1, 2 and 4 mg x kg(-1). The pharmacokinetic study of Mit-lipo and Mit-free was performed on dogs following a single intravenous injection.

A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of pinaverium bromide in human plasma: application to a pharmacokinetic study in healthy volunteers.

A sensitive and specific method using liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) for the determination of pinaverium bromide in human plasma was developed and validated. Pinaverium bromide and an internal standard (paclitaxel) were isolated from plasma samples by precipitating plasma, and determined by LC-MS/MS in multiple-reaction monitoring mode. The main metabolite of pinaverium bromide and endogenous substances in plasma did not show any interference.

Highly selective detection of phosphate in very complicated matrixes with an off-on fluorescent probe of europium-adjusted carbon dots.

A simple method for phosphate (Pi) detection is established by developing an off-on fluorescence probe of europium-adjusted carbon dots (CDs), which has been successfully applied to the detection of Pi in very complicated matrixes such as artificial wetlands system.

A theoretical study of the mechanism for the homogeneous catalytic reversible dehydrogenation-hydrogenation of nitrogen heterocycles.

A mechanism for the dehydrogenation reaction of 1,2,3,4-tetrahydroquinoline to quinoline derivatives, catalyzed by a Cp*Ir complex containing a 2-pyridonate ligand, is proposed and supported by theoretical calculations at the B3LYP level. The proposed mechanism involves two stages which are all thermodynamically unfavorable (endothermic by 36.3 kcal mol(-1) and 18.

Intratumoral IL-12 gene therapy results in the crosspriming of Tc1 cells reactive against tumor-associated stromal antigens.

HLA-A2 transgenic mice bearing established HLA-A2(neg) B16 melanomas were effectively treated by intratumoral (i.t.) injection of syngeneic dendritic cells (DCs) transduced to express high levels of interleukin (IL)-12, resulting in CD8(+) T cell-dependent antitumor protection.

Evaluation of manganese peroxidase (MnP) for its ability to resist the ozonization and thereafter decolorize methyl orange.

The goal of this study was to determine whether ozone can be used to suppress bacterial growth in operating a white rot fungi reactor system. The effects of ozone dose on the activity of manganese peroxidase (MnP) and on the death rate of Escherichia coli were investigated. The results showed that at ozone dose of 0.

Releasing of the chromophore from the drug delivery protein C-1027: a molecular dynamics simulations study.

The aromatized chromophore (Chr) of C-1027 with selective DNA-cleaving ability, which is stabilized and delivered by the apoprotein (Apo) in vitro, is not released until the holoprotein (Apo+Chr) penetrates into the cultured cancer cells. As a drug delivery system, the holoprotein has gained much attention in clinical application. However, the Chr-releasing mechanism is ambiguous so far.

A one-pot strategy for biomimetic synthesis and self-assembly of gold nanoparticles.

A simple, one-pot and controllable strategy is reported in this contribution for biomimetic synthesis and self-assembly of gold nanoparticles (Au-NPs). It involves our synthesized polyaldehyde dextran (PAD), which has been proved to be a biomacromolecule with excellent biocompatibility and biodegradability, acting as both a reducing agent and a stabilizer. The morphology of the as-prepared Au-NP assemblies can be controlled by adjusting the reaction conditions, such as the concentration of aldehyde in PAD, the reaction time and the temperature.

[A structural mechanics study with human maxillary premolar].

Objective: To evaluate the stress distribution and offset of dental and periodontal tissues imposed by changes in inner diameter of pulp cavity.

Methods: Six models of maxillary second bicuspid tooth with different inner diameter of pulp cavity were established, including: (1) calcificated pulp without pulp chamberi (2) mostly calcificated pulp chamber with inward reduction of 0. 5 mm in normal pulp cavity; (3) initially calcificated pulp chamber with inward reduction of 0.

Alu-directed transcriptional regulation of some novel miRNAs.

Background: Despite many studies on the biogenesis, molecular structure and biological functions of microRNAs, little is known about the transcriptional regulatory mechanisms controlling the spatiotemporal expression pattern of human miRNA gene loci. Several lines of experimental results have indicated that both polymerase II (Pol-II) and polymerase III (Pol-III) may be involved in transcribing miRNAs. Here, we assessed the genomic evidence for Alu-directed transcriptional regulation of some novel miRNA genes in humans.

The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.

The retromer is a protein complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network (TGN). It is comprised of a cargo-selection subcomplex of Vps26, Vps29 and Vps35 and a membrane-binding coat subcomplex of sorting nexins (SNXs). Previous studies identified SNX1/2 as one of the components of the SNX subcomplex, and SNX5/6 as candidates for the second SNX.

IL-4 suppresses very late antigen-4 expression which is required for therapeutic Th1 T-cell trafficking into tumors.

Murine CD4 T cells cultured under type 1 polarizing conditions selectively express significantly higher levels of the very late antigen (VLA)-4 and VLA-6 integrins when compared with T cells cultured under type 2 or nonpolarizing (type 0) conditions. This difference appears due to the action of interleukin (IL)-4, as loss of VLA-4/-6 expression on Th cells was prevented by inclusion of neutralizing anti-IL-4 mAb during the initial culture period. We also observed that CD4 T cells deficient in Stat6, a critical component of the IL-4R signaling cascade, retained high levels of VLA-4 and VLA-6 expression, regardless of IL-4 status in the culture conditions.

Molecular dynamics simulations investigation of neocarzinostatin chromophore-releasing pathways from the holo-NCS protein.

The enediyne ring chromophore with strong DNA cleavage activity of neocarzinostatin is labile and therefore stabilization by forming the complex (carrying protein+chromophore: holo-NCS). Holo-NCS has gained much attention in clinical use as well as for drug delivery systems, but the chromophore-releasing mechanism to trigger binding to the target DNA with high affinity and producing DNA damage remain unclear. Three possible pathways were initially determined by conventional MD, essential dynamics and essential dynamics sampling.

Ultrasonic-assisted precolumn derivatization-HPLC determination of acrylamide formed in Radix Asparagi during heating process.

An ultrasonic-assisted precolumn derivatization-HPLC method was established and validated for the determination of acrylamide formed in traditional Chinese herb Radix Asparagi during heating process. This method entails extraction with water, ultrasonic-assisted derivatization with 2-mercaptobenzoic acid. The final extracted acrylamide derivative was separated on C(18) column by using a mixture of acetonitrile and acetic acid (1 gL(-1) water solution) (20:80, v/v) as mobile phase.

CD8+ T-cell responses against hemoglobin-beta prevent solid tumor growth.

Bone marrow-derived dendritic cells engineered using recombinant adenovirus to secrete high levels of IL-12p70 dramatically inhibited the growth of established CMS4 sarcomas in BALB/c mice after intratumoral administration. An analysis of splenic CD8(+) T cells in regressor mice revealed a strong, complex reactivity pattern against high-performance liquid chromatography (HPLC)-resolved peptides isolated by acid elution from single-cell suspensions of surgically resected CMS4 lesions. Mass spectrometry analyses defined two major overlapping peptide species that derive from the murine hemoglobin-beta (HBB) protein within the most stimulatory HPLC fractions.