Cellular and humoral immune responses in cats vaccinated with feline herpesvirus 1 modified live virus vaccine.

Feline herpesvirus 1 (FHV-1) is an important pathogen causing infectious rhinotracheitis in felids, mainly infecting the upper respiratory tract and conjunctiva. Multiple vaccines are available to prevent FHV-1 infection, and the antibody levels are always used to evaluate their effectiveness. However, the cellular immunity response following immunization in cats remains unclear.

Revisiting the female germline cell development.

The formation of the female germline is the fundamental process in most flowering plants' sexual reproduction. In , only one somatic cell obtains the female germline fate, and this process is regulated by different pathways. Megaspore mother cell (MMC) is the first female germline, and understanding MMC development is essential for comprehending the complex mechanisms of plant reproduction processes.

Terahertz programmable metasurface based on solid-state plasma.

Terahertz modulation technology based on programmable metasurfaces can respond in real time to external signals or environmental changes, enabling flexible and adaptive control of terahertz waves. This technology demonstrates significant potential and importance across various fields, including communication, imaging, scientific research, security monitoring, and industrial applications. This paper proposes a terahertz programmable metasurface based on solid-state plasma, utilizing solid-state plasma devices to achieve dynamic control of properties such as the amplitude and phase of terahertz waves.

Newly isolated bacterium and arbuscular mycorrhizal fungus effectively reduce the root cadmium concentration and increase the root biomass of Ophiopogon japonicus.

Soil cadmium (Cd) contamination is one of the major challenges in food production. This has led to above-maximum threshold accumulation of Cd in O. japonicus roots.

Targeted Next-Generation Sequencing in Pneumonia: Applications in the Detection of Responsible Pathogens, Antimicrobial Resistance, and Virulence.

Background: Targeted next-generation sequencing (tNGS) is a high-throughput and cost-effective diagnostic alternative for pneumonia, with the ability to simultaneously detect pathogens, antimicrobial resistance genes, and virulence genes. We aimed to explore the applicability of tNGS in the co-detection of the responsible pathogens, antimicrobial resistance (AMR) genes, and virulence genes in patients with pneumonia.

Methods: A prospective study was conducted among patients with suspected pneumonia at Ruijin Hospital from March 1 to May 31, 2023.

Genomic and Metabolomic Analyses of with Enhanced ε-Poly-l-lysine Production Through Adaptive Laboratory Evolution.

ε-poly-l-lysine (ε-PL), a natural food preservative, has garnered widespread attention. It is mainly produced by , but the production by wild-type strains fails to meet the demands of industrialization. To address this issue, adaptive laboratory evolution (ALE) was successfully employed in this study, subjecting CICC 11022 to environmental stresses such as acidic pH and antibiotics (rifampicin, gentamicin, and streptomycin).

[Clinical efficacy of open reduction and internal fixation with plates versus minimally invasive Kirschner wire fixation for osteoporotic Colles' fractures].

Objective: To compare the short-term clinical efficacy and safety of closed reduction with Kirschner wire fixation versus open reduction with plate fixation for treating osteoporotic Colles' fractures in middle-aged and elderly patients.

Methods: Between January 2018 and January 2023, 119 patients with Colles fractures were retrospectively analyzed, including 39 males and 80 females, aged from 48 to 74 years old with an average of(60.58±6.

Dedicator of Cytokinesis 2 regulates cytoskeletal actin dynamics and is essential for platelet biogenesis and functions.

Aims: Dedicator of Cytokinesis 2 (DOCK2), a member of the DOCK family of Guanine nucleotide exchange factors that specifically act on the Rho GTPases including Rac and Cdc42, plays pivotal roles in the regulation of leukocyte homeostasis. However, its functions in platelets remain unknown.

Methods And Results: Using mice with genetic deficiency of DOCK2 (Dock2-/-), we showed that Dock2-/-mice exhibited a macrothrombocytopenic phenotype characterized as decreased platelet count and enlarged platelet size by transmission electron microscopy.

Dual-modal radiomics ultrasound model to diagnose cervical lymph node metastases of differentiated thyroid carcinoma: a two-center study.

Objectives: To establish and validate a dual-modal radiomics nomogram from grayscale ultrasound and color doppler flow imaging (CDFI) of cervical lymph nodes (LNs), aiming to improve the diagnostic accuracy of metastatic LNs in differentiated thyroid carcinoma (DTC).

Methods: DTC patients with suspected cervical LNs in two medical centers were retrospectively enrolled. Pathological results were set as gold standard.

MYBL2 promotes proliferation of clear cell renal cell carcinoma by regulating TOP2A and activating AKT/mTOR signaling pathway.

Renal cell carcinoma (RCC) is one of the most common malignancies in the urinary system, and clear cell renal cell carcinoma (ccRCC) is the most common subtype. MYBL2 has been reported to be overexpressed in various tumors and associated with poor prognosis in patients, but its biological role in ccRCC remains unclear. In this study, we investigated the mRNA and protein expression levels of MYBL2 in ccRCC samples and evaluated the prognostic value of MYBL2 using TCGA dataset.

Risk Factors for Disseminated Tuberculosis and Associated Survival in Adults Without Human Immunodeficiency Virus.

Background: The global resurgence of disseminated tuberculosis (TB) after the coronavirus disease 2019 pandemic highlights the necessity of understanding host risk factors, especially in adults without human immunodeficiency virus.

Methods: We reviewed TB cases admitted to Shanghai Public Health Clinical Center from 2017 to 2022. We analyzed baseline characteristics and outcomes.

Proteolysis-targeting influenza vaccine strains induce broad-spectrum immunity and in vivo protection.

Generating effective live vaccines from intact viruses remains challenging owing to considerations of safety and immunogenicity. Approaches that can be applied in a systematic manner are needed. Here we created a library of live attenuated influenza vaccines by using diverse cellular E3 ubiquitin ligases to generate proteolysis-targeting (PROTAR) influenza A viruses.

PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins.

Manipulating viral protein stability using the cellular ubiquitin-proteasome system (UPS) represents a promising approach for developing live-attenuated vaccines. The first-generation proteolysis-targeting (PROTAR) vaccine had limitations, as it incorporates proteasome-targeting degrons (PTDs) at only the terminal ends of viral proteins, potentially restricting its broad application. Here we developed the next-generation PROTAR vaccine approach, referred to as PROTAR 2.

Retrieving the Stability and Practical Performance of Activation-Unstable Mesoporous Zr(IV)-MOF for Highly Efficient Self-Calibrating Acidity Sensing.

The practical applications of activation-unstable mesoporous metal-organic frameworks (MOFs) are often constrained by their structural instability. However, enhancing their stability could unlock valuable functionalities. Herein, we stabilized the otherwise unstable, post-activated structure of a novel mesoporous Zr(IV)-MOF, NKM-809, which constructed from a pyridine-containing amphiprotic linker (PPTB).

Comparison of Jinzhen oral liquid and ambroxol hydrochloride and clenbuterol hydrochloride oral solution in the treatment of acute bronchitis in children: A multicenter, non-inferiority, prospective, randomized controlled trial.

The comparison between traditional Chinese medicine Jinzhen oral liquid (JZOL) and Western medicine in treating children with acute bronchitis (AB) showed encouraging outcomes. This trial evaluated the efficacy and safety of the JZOL for improving cough and expectoration in children with AB. 480 children were randomly assigned to take JZOL or ambroxol hydrochloride and clenbuterol hydrochloride oral solution for 7 days.

Preparation of Molecularly Imprinted Electrochemical Sensors and Analysis of the Doping of Epinephrine in Equine Blood.

In this paper, a novel molecularly imprinted polymer membrane modified glassy carbon electrode for electrochemical sensors (MIP-OH-MWCNTs-GCE) for epinephrine (EP) was successfully prepared by a gel-sol method using an optimized functional monomer oligosilsesquioxane-AlO sol-ITO composite sol (ITO-POSS-AlO). Hydroxylated multi-walled carbon nanotubes (OH-MWCNTs) were introduced during the modification of the electrodes, and the electrochemical behavior of EP on the molecularly imprinted electrochemical sensors was probed by the differential pulse velocity (DPV) method. The experimental conditions were optimized.

Association of Craniofacial Skeletal and Soft Tissue Characteristics With Severe Obstructive Sleep Apnea in Age-specific and BMI-specific Patient Groups.

Purpose: To identify the key craniofacial anatomic characteristics associated with the prevalence of severe obstructive sleep apnea (OSA) in patient cohorts stratified by age and body mass index (BMI).

Methods: This prospective study was conducted at the Beijing Anzhen Hospital Center for Sleep Medicine and Science between December 2023 and March 2024. Patients suspected of having OSA underwent overnight polysomnography, along with computed tomography scans of the head and neck, to evaluate the skeletal and soft tissue characteristics.

Photonic axion insulator.

Axions, hypothetical elementary particles that remain undetectable in nature, can arise as quasiparticles in three-dimensional crystals known as axion insulators. Previous implementations of axion insulators have largely been limited to two-dimensional systems, leaving their topological properties in three dimensions unexplored in experiment. Here, we realize an axion insulator in a three-dimensional photonic crystal and probe its topological properties.

Clinical application of targeted next-generation sequencing utilizing bronchoalveolar lavage fluid in thoracic surgery ICU patients with suspected pulmonary infections.

Aims: The aim of this prospective study was to evaluate the diagnostic value of targeted next-generation sequencing (tNGS) in identifying pathogens from bronchoalveolar lavage fluid (BALF) in thoracic surgery ICU patients, offering additional diagnostic methods for clinical practice.

Methods And Results: We collected clinical data from patients with suspected pulmonary infections in the thoracic surgery ICU of the Second Affiliated Hospital of Air Force Medical University. A total of 50 patients were enrolled in this study.

Effect modification of the association between vitamin B2 intake and diabetes mellitus by sex: findings from the National Health and Nutrition Examination Survey 2013-2020.

Background And Objectives: The relationship between vitamin intake and diabetes mellitus (DM) has attracted growing attention. Only few studies have linked vitamin B2 (VB2) and development of DM. In this study, we aimed to assess the association between VB2 intake and DM among U.

Associations of baseline and longitudinal changes in basic activity of daily living with risk of cardiovascular disease among older adults in China.

Background And Aims: The purpose of this study was to examine the associations of basic activities of daily living (BADL) and its longitudinal changes with cardiovascular disease (CVD) risk among older population.

Methods And Results: We conducted a prospective analysis of the Chinese Longitudinal Healthy Longevity Survey between 2008 and 2018 in 7051 participants aged 65 years or over. Cox proportional hazards models were performed to evaluate the associations of baseline and longitudinal changes in BADL with the incidence of CVD.

Targeting T cell exhaustion: emerging strategies in non-small cell lung cancer.

Lung cancer continues to be a major contributor to cancer-related deaths globally. Recent advances in immunotherapy have introduced promising treatments targeting T cell functionality. Central to the efficacy of these therapies is the role of T cells, which are often rendered dysfunctional due to continuous antigenic stimulation in the tumor microenvironment-a condition referred to as T cell exhaustion.

CD8 T cell exhaustion in the tumor microenvironment of breast cancer.

CD8+ T cells are crucial cytotoxic components of the tumor immune system. In chronic inflammation, they become low-responsive, a state known as T cell exhaustion (TEX). The aim of immune checkpoint blockade is to counteract TEX, yet its dynamics in breast cancer remain poorly understood.

A reconstruction method for ptychography based on residual dense network.

Background: Coherent diffraction imaging (CDI) is an important lens-free imaging method. As a variant of CDI, ptychography enables the imaging of objects with arbitrary lateral sizes. However, traditional phase retrieval methods are time-consuming for ptychographic imaging of large-size objects, e.