Innovative Insights into Single-Cell Technologies and Multi-Omics Integration in Livestock and Poultry.

In recent years, single-cell RNA sequencing (scRNA-seq) has marked significant strides in livestock and poultry research, especially when integrated with multi-omics approaches. These advancements provide a nuanced view into complex regulatory networks and cellular dynamics. This review outlines the application of scRNA-seq in key species, including poultry, swine, and ruminants, with a focus on outcomes related to cellular heterogeneity, developmental biology, and reproductive mechanisms.

Modeling multi-stage disease progression and identifying genetic risk factors via a novel collaborative learning method.

Motivation: Alzheimer's disease (AD) typically progresses gradually for ages rather than suddenly. Thus, staging AD progression in different phases could aid in accurate diagnosis and treatment. In addition, identifying genetic variations that influence AD is critical to understanding the pathogenesis.

Toxicological effects and molecular metabolic of polystyrene nanoplastics on soybean (Glycine max L.): Strengthening defense ability by enhancing secondary metabolisms.

Nanoplastics, as emerging pollutants, have attracted worldwide concern for their possible environmental dangers. The ingestion and accumulation of nanoplastics in crops can contaminate the food chain and have unintended consequences for human health. In this study, we revealed the effects of polystyrene nanoplastics (PS-NPs; 80 nm) at different concentrations (0, 10, 100 mg L) on soybean (Glycine max L.

Prevalence of bone destruction in patients with Talaromyces marneffei infection: A systematic review and meta-analysis.

Objectives: This systematic review and meta-analysis aimed to evaluate the prevalence of bone destruction in patients with Talaromyces marneffei infection, examine distribution patterns of bone lesions, and assess differences between HIV-positive and HIV-negative patients.

Methods: Following PRISMA guidelines, 15 studies involving 839 patients were analyzed. Random-effects meta-analysis was performed to estimate prevalence and odds ratios.

Prevalence and genotypic characterization of zoonotic intestinal protozoan parasites in transboundary migratory Mongolian Gazelles (): the first survey.

The migration of Mongolian gazelles () poses a potential risk of outbreak for zoonotic intestinal protozoan parasite infections. This study aims to investigate the infection status of zoonotic intestinal protozoan parasites in these migratory Mongolian gazelles. We collected 120 fecal samples from Mongolian gazelles during their migration from Mongolia to China in December 2023.

Polyphyllin VI: A promising treatment for prostate cancer bone metastasis.

Prostate cancer, as one of the most prevalent malignant tumors in men, seriously affects the prognosis and survival of patients due to its extremely high rate of bone metastasis. This study investigated the effect of Polyphyllin VI (PPVI) on metastatic bone disease for the first time in prostate cancer, focusing on its impact on osteoclast and tumor cell. In vitro studies utilized TRAP staining, ghost pen cyclic peptide staining, and bone resorption assays to evaluate the differentiation and function of receptor activator of nuclear factor-κB ligand (RANKL) induced and RM-1 conditional medium (CM) induced osteoclasts.

Effects of Different Dietary Combinations on Blood Biochemical Indicators and Rumen Microbial Ecology in Wenshan Cattle.

With the continuous optimization of feed ingredients in livestock production, barley has garnered significant attention as a potential substitute for corn in feed. This study aims to investigate the effects of replacing part of the corn and soybean meal with barley, wheat bran, and rapeseed meal on Wenshan cattle, focusing on the rumen microbial community, blood physiological and biochemical indicators, and growth traits. Through an intensive feeding experiment with two different dietary ratios, we found that adding barley to the diet significantly reduced the host's blood lipid concentration and significantly increased the height, body length, heart girth, and average daily weight gain of Wenshan cattle.

LUT-based phase error compensation method for large-step phase-shifting algorithm in DLP4500-based FPP system.

As a low-cost professional digital light projection device, the DLP4500 have been widely applied in fringe projection profilometry (FPP), for both laboratory and practical application. However, our recent experiments revealed a new hardware-induced projection instability when the projection pattern data exceeds its buffer capacity (48 bits). This phenomenon undermines the measurement accuracy advantage of the phase-shifting (PS) algorithms with large number of shifting steps, and eventually leads unwanted and complicated error to 3D reconstruction.

Using fungal-bacterial community analysis to explore potential microbiomes to manage .

Rhizosphere microbial communities strongly affect outbreaks of root-knot nematode (RKN) disease. However, little is known about the interactions among fungi, bacteria and RKN. The bacterial and fungal community compositions in the rhizospheres of four representative tobacco varieties, both resistant and susceptible to RKN, were characterized using 16S rRNA gene sequencing for bacteria and internal transcribed spacer gene sequencing for fungi.

Pressure-Induced Structural Phase Transitions and Photoluminescence Properties of Micro/Nanocrystals HoF.

HoF crystals of varying sizes and morphologies were synthesized by controlling the amount of water. As the water content gradually decreased, the size of the crystals reduced, transforming from microcrystals to approximately 100 nm nanocrystals with unique morphologies. At room temperature, the pressure-induced phase transitions, and photoluminescence (PL) properties of HoF micro/nanocrystals are studied using in situ high-pressure PL technique.

Feasibility study of YSO/SiPM based detectors for virtual monochromatic image synthesis.

Background: The development of photon-counting CT systems has focused on semiconductor detectors like cadmium zinc telluride (CZT) and cadmium telluride (CdTe). However, these detectors face high costs and charge-sharing issues, distorting the energy spectrum. Indirect detection using Yttrium Orthosilicate (YSO) scintillators with silicon photomultiplier (SiPM) offers a cost-effective alternative with high detection efficiency, low dark count rate, and high sensor gain.

Nucleic Acid-Based Biological Nanopore Sensing Strategies for Tumor Marker Detection.

Cancer, which is characterized by high mortality rates, poses a significant threat to global human health. Early diagnosis is of paramount importance in managing cancer, and tumor markers have emerged as crucial indicators for achieving this goal. The advent of precision medicine has further emphasized the need for the effective detection of these markers.

Application of Pan-Omics Technologies in Research on Important Economic Traits for Ruminants.

The economic significance of ruminants in agriculture underscores the need for advanced research methodologies to enhance their traits. This review aims to elucidate the transformative role of pan-omics technologies in ruminant research, focusing on their application in uncovering the genetic mechanisms underlying complex traits such as growth, reproduction, production performance, and rumen function. Pan-omics analysis not only helps in identifying key genes and their regulatory networks associated with important economic traits but also reveals the impact of environmental factors on trait expression.

Application of GWAS and mGWAS in Livestock and Poultry Breeding.

In recent years, genome-wide association studies (GWAS) and metabolome genome-wide association studies (mGWAS) have emerged as crucial methods for investigating complex traits in animals and plants. These have played pivotal roles in research on livestock and poultry breeding, facilitating a deeper understanding of genetic diversity, the relationship between genes, and genetic bases in livestock and poultry. This article provides a review of the applications of GWAS and mGWAS in animal genetic breeding, aiming to offer reference and inspiration for relevant researchers, promote innovation in animal genetic improvement and breeding methods, and contribute to the sustainable development of animal husbandry.

Impact of gender, race, and age of onset on the phenotype and comorbidities of pediatric eosinophilic esophagitis.

Objectives: To investigate differences in symptoms, allergy comorbidities, and eosinophilic inflammation at the time of diagnosis for patients with eosinophilic esophagitis (EoE) based on gender, race, and age of onset.

Methods: A retrospective study was conducted at a multidisciplinary EoE clinic; the correlation between histological findings, previously identified symptoms, associated comorbidities, and demographics including gender, race, as well as age of onset was examined. Chi-squared and Student's -tests were utilized for statistical analysis.

Feasibility study of photon-counting CT for material identification based on YSO/SiPM detector: A proof of concept.

Background: Current photon-counting computed tomography (CT) systems utilize semiconductor detectors, such as cadmium telluride (CdTe), cadmium zinc telluride (CZT), and silicon (Si), which convert x-ray photons directly into charge pulses. An alternative approach is indirect detection, which involves Yttrium Orthosilicate (YSO) scintillators coupled with silicon photomultipliers (SiPMs). This presents an attractive and cost-effective option due to its low cost, high detection efficiency, low dark count rate, and high sensor gain.

Exploring the additive effect of Ampelopsis grossedentata flavonoids and Tween 80 on feeding Nubian goats.

Introduction: The ban on antibiotics in animal husbandry underscores the crucial need for safe, natural feed additives. This study investigates the effects of Ampelopsis grossedentata flavonoids (AGF) and Tween 80 on the growth performance, blood indexes, and rumen microbiota of Nubian goats, evaluating their potential as alternative feed additives in livestock management.

Methods: Thirty-two goats were randomly divided into four groups.

The miR172/TOE3 module regulates resistance to tobacco mosaic virus in tobacco.

The outcome of certain plant-virus interaction is symptom recovery, which is accompanied with the emergence of asymptomatic tissues in which the virus accumulation decreased dramatically. This phenomenon shows the potential to reveal critical molecular factors for controlling viral disease. MicroRNAs act as master regulators in plant growth, development, and immunity.

Beef Cattle Genome Project: Advances in Genome Sequencing, Assembly, and Functional Genes Discovery.

Beef is a major global source of protein, playing an essential role in the human diet. The worldwide production and consumption of beef continue to rise, reflecting a significant trend. However, despite the critical importance of beef cattle resources in agriculture, the diversity of cattle breeds faces severe challenges, with many breeds at risk of extinction.

Methyl jasmonate-induced bHLH42 mediates tissue-specific accumulation of anthocyanins via regulating flavonoid metabolism-related pathways in Caitai.

Anthocyanin is a type of plant secondary metabolite beneficial to human health. The anthocyanin content of vegetable and fruit crops signifies their nutritional quality. However, the molecular mechanism of anthocyanin accumulation, especially tissue-specific accumulation, in Caitai, as well as in other Brassica rapa varieties, remains elusive.