Intratracheal Inoculation of Fischer 344 Rats with Francisella tularensis.

Pulmonary infection with the bacterium Francisella tularensis can lead to the serious and potentially fatal disease, tularemia, in humans. Due to the current lack of an approved tularemia vaccine for humans, research is focused on vaccine development utilizing appropriate animal models. The Fischer 344 rat has emerged as a model that reflects human susceptibility to F.

Lipidation of the FPI protein IglE contributes to Francisella tularensis ssp. novicida intramacrophage replication and virulence.

Francisella tularensis is a Gram-negative bacterium responsible for the human disease tularemia. The Francisella pathogenicity island (FPI) encodes a secretion system related to type VI secretion systems (T6SS) which allows F. tularensis to escape the phagosome and replicate within the cytosol of infected macrophages and ultimately cause disease.

Draft Genome Sequence of the Fish Pathogen Piscirickettsia salmonis.

Piscirickettsia salmonis is a Gram-negative intracellular fish pathogen that has a significant impact on the salmon industry. Here, we report the genome sequence of P. salmonis strain LF-89.

Cyclic di-GMP stimulates biofilm formation and inhibits virulence of Francisella novicida.

Francisella tularensis is a gram-negative bacterium that is highly virulent in humans, causing the disease tularemia. F. novicida is closely related to F.

Evasion of IFN-γ signaling by Francisella novicida is dependent upon Francisella outer membrane protein C.

Background: Francisella tularensis is a Gram-negative facultative intracellular bacterium and the causative agent of the lethal disease tularemia. An outer membrane protein (FTT0918) of F. tularensis subsp.

A bacterial two-hybrid system that utilizes Gateway cloning for rapid screening of protein-protein interactions.

Comprehensive clone sets representing the entire genome now exist for a large number of organisms. The Gateway entry clone sets are a particularly useful means to study gene function, given the ease of introduction into any Gateway-suitable destination vector. We have adapted a bacterial two-hybrid system for use with Gateway entry clone sets, such that potential interactions between proteins encoded within these clone sets can be determined by new destination vectors.

Genetic manipulation of francisella tularensis.

Francisella tularensis is a facultative intracellular pathogen that causes the disease tularemia. F. tularensis subsp.

Characterization of the Francisella tularensis subsp. novicida type IV pilus.

Francisella tularensis causes the disease tularaemia. Type IV pili (Tfp) genes are present in the genomes of all F. tularensis subspecies.

Construction of targeted insertion mutations in Francisella tularensis subsp. novicida.

Francisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp.

Occurrence and regulation of the multicellular morphotype in Salmonella serovars important in human disease.

Multicellular behavior in Salmonella Typhimurium ATCC14028 called the rdar morphotype is characterized by the expression of the extracellular matrix components cellulose and curli fimbriae. Over 90% of S. Typhimurium and S.

Production of cellulose and curli fimbriae by members of the family Enterobacteriaceae isolated from the human gastrointestinal tract.

Citrobacter spp., Enterobacter spp., and Klebsiella spp.