Publications by authors named "Xavier O Weenink"
Article Synopsis
- Mannosyltransferases in the Golgi add mannose residues to glycoproteins and glycolipids, relying on GDP-mannose transported by a specific glycoprotein transporter.
- Researchers identified the gmtA gene in Aspergillus niger as the sole GDP-mannose transporter, finding that it is essential for survival and can be functionally complemented by a YFP-GmtA protein fusion.
- The srgC gene, necessary for Golgi to vacuole transport, is crucial for maintaining Golgi structure in A. niger; its disruption causes reduced growth and loss of Golgi-like structures.
A new method for screening and isolation of hypersecretion mutants in Aspergillus niger.
Appl Microbiol Biotechnol
February 2006
Although filamentous fungi have a unique property of secreting a large amount of homologous extracellular proteins, the use of filamentous fungi as hosts for the production of heterologous proteins is limited because of the low production levels that are generally reached. Here, we report a general screening method for the isolation of mutants with increased protein production levels. The screening method makes use of an Aspergillus niger strain that lacks the two major amylolytic enzymes, glucoamylase (GlaA) and acid amylase (AamA).
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