Isolation and characterization of mesenchymal stem cells from the fat layer on the density gradient separated bone marrow.

The density gradient centrifugation method was originally designed for the isolation of mononuclear peripheral blood cells and rapidly adapted to fractionate bone marrow (BM) cells. This method involves the use of gradient density solutions with low viscosity and low osmotic pressure that allows erythrocytes and more mature cells gravitate to the bottom at a density fraction superior to 1.080 g/dL; mononuclear cells (MNCs) held in the plasma-solution to interphase at a density between 1.

Quantitative analysis of the factors that affect the determination of colocalization coefficients in dual-color confocal images.

Colocalization analysis is a subject of great interest that allows us to determine those locations at which two or more types of molecules or structures are found simultaneously. In confocal dual-color images, in order to consider a given position as colocalized, the following two conditions must be satisfied: 1) the colors emitted by the molecules labeled with different fluorophores must occupy the same pixel in the image and 2) the intensities of each component of the image must be within a certain range. Since it is not straightforward to assess these two conditions without ambiguity, this can lead to either considering false colocalizations as positive, or, conversely, leaving out positive locations.