Publications by authors named "Xavier Benigni"
microRNA (miRNA) expression profiles based on the highly powerful Illumina sequencing technology rely on the construction of cDNA libraries in which adaptor ligation is known to deeply favor some miRNAs over others. This introduces erroneous measurements of the miRNA abundances and relative miRNA quantities in biological samples. Here, by using the commercial miRXplore Universal Reference that contains an equimolar mixture of 963 animal miRNAs and TruSeq or bulged adaptors, we describe a method for correcting ligation biases in expression profiles obtained with standard protocols of cDNA library construction and provide data for quantifying the true miRNA abundances in biological samples.
View Article and Find Full Text PDFEpidemiological reports and studies using rodent models indicate that early exposure to nutrient and/or hormonal challenges can reprogram metabolism at adulthood. Hypothalamic arcuate nucleus (ARC) integrates peripheral and central signals to adequately regulate energy homeostasis. microRNAs (miRNAs) participate in the control of gene expression of large regulatory networks including many signaling pathways involved in epigenetics regulations.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates the role of microRNAs (miRNAs) in regulating gene expression during the development of neuron networks in a specific area of the rat brain called the arcuate nucleus and median eminence (ARC/ME).
- - Researchers analyzed over 300 miRNAs at various postnatal stages and found significant changes in expression, particularly between the ages of P8 to P14 and P21 to P28, highlighting the dynamic nature of miRNA levels during brain development.
- - The offspring of rats fed an unbalanced diet showed growth issues and lower leptin levels but retained similar miRNA expression patterns, suggesting that nutrition during gestation and lactation affects development while allowing certain miRNA profiles to remain consistent.