A New qPCR Assay for the Rapid Diagnosis of Subspecies.

Rapid and accurate identification of subspecies is crucial for effective management and eradication. Current diagnostic methods have limitations in terms of time to diagnosis (up to seven days) and can yield ambiguous results. Here, we present the validation of a custom TaqMan SNP Genotyping Assay for the rapid and accurate identification of (boll weevil) and (thurberia weevil) subspecies.