Front Bioeng Biotechnol
August 2024
Currently, Gramicidin S (GR-S) is produced enzymatically with the drawback of the presence of trifluoroacetic acid (TFA) or produced by fermentation involving several separation and purification steps. Therefore, this study is focused on the use of green solvents as unique extraction step to produce Gramicidin S from microbial biomass of Among the tested solvents, such as ethanol, acidic ethanol or buffer phosphate, the most favorable was acidic ethanol, extracting 96% of Gramicidin S from cells with a purity of 90%. Using acidic ethanol, extraction time within the range of 30-120 min exhibited minimal impact on Gramicidin S yield, whereas the biomass-to-extractant ratio emerged as a critical parameter.
View Article and Find Full Text PDFThis study evaluates a hybrid system combining zeolites as a sorption stage and a hollow fiber membrane contactor (HFMC) for ammonia (NH) recovery from treated urban wastewater. Ion exchange with zeolites was selected as an advanced pretreatment and concentration step before the HFMC. The system was tested with wastewater treatment plant (WWTP) effluent (mainstream, 50 mg N-NH/L) and anaerobic digestion centrates (sidestream, 600-800 mg N-NH/L) from another WWTP.
View Article and Find Full Text PDFIn this work, we assessed the effect of a biosurfactant extract, which possesses preservative properties, on the sensory properties, regarding colour, of two fruit juices: pasteurized apple juice and natural orange juice. This biosurfactant extract was obtained from corn steep liquor, a secondary stream of the corn wet-milling industry. The biosurfactant extract is composed of natural polymers and biocompounds released during the spontaneous fermentation of corn kernels during the steeping process of corn.
View Article and Find Full Text PDFMembrane contactor is a promising technology for ammonia recovery from the anaerobic digestion centrate. However, high suspended solids and dissolved organic matter concentrations can reduce the effectiveness of the technology. In this study, coagulation-flocculation (C/F) and aeration pre-treatments were evaluated to reduce chemical oxygen demand (COD), turbidity, suspended solids and alkalinity before the ammonia recovery stage using a membrane contactor.
View Article and Find Full Text PDFBiosurfactant extracts are multifunctional ingredients composed of natural polymers that can be used in the food industry as stabilizing and antimicrobial agents, although their inclusion in food matrices has been scarcely explored. In this work, a biosurfactant extract, with antimicrobial properties, obtained from a fermented stream of the corn wet-milling industry was introduced into an apple and orange juice matrix to evaluate the changes produced in the sugar consumption, pH, and biomass formation at different temperatures (4-36 °C) and storage time (1-7 days). It was observed that the addition of biosurfactant extract reduced the hydrolysis rate of polymeric sugars, decreasing the concentration of soluble sugars from 85.
View Article and Find Full Text PDFSpinach and orange by-products are well recognized for their health benefits due to the presence of natural polyphenols with antioxidant activity. Therefore, the demand to produce functional products containing polyphenols recovered from vegetables and fruits has increased in the last decade. This work aims to use the integrated membrane process for the recovery of polyphenols from spinach and orange wastes, implemented on a laboratory scale.
View Article and Find Full Text PDFLiquid-liquid membrane contactors (LLMCs) were studied as a sustainable technology for ammonia recovery from wastewater. Ammonia can be valorized by LLMCs as a potential nutrient and produce liquid fertilizers. Thus, this work aims for the study of different experimental LLMC conditions to produce ammonium salts by an acid stripping stream.
View Article and Find Full Text PDFThe aim of this study was to evaluate the recovery of phenolic compounds from olive mill and winery wastes by conventional solid-liquid extraction (SLE) using water as the extraction solvent. The studied variables were extraction time (5-15 min), temperature (25-90 °C), solid-to-liquid ratio (1:10-1:100 (kg/L)), pH (3-10) and application of multiple extractions (1-3). The extraction efficiency was evaluated in terms of total phenolic content (TPC), determined by high performance liquid chromatography (HPLC-UV), but also from the recovery of some representative phenolic compounds.
View Article and Find Full Text PDFMore sustainable waste management in the winery and olive oil industries has become a major challenge. Therefore, waste valorization to obtain value-added products (e.g.
View Article and Find Full Text PDFThe production of olive oil and wine are two of the main agri-food economic activities in Southern Europe. They generate large amounts of solid and liquid wastes (e.g.
View Article and Find Full Text PDFInjection of zero-valent iron nanoparticles (nZVI) into aquifers has gained increasing attention of researchers for in-situ treatment of NO-contaminated groundwater. nZVI has proved efficient in chemically reducing NO and, according to recent research efforts, in supporting biological denitrification under favoured conditions. Given the scarce research on nZVI pulsed injection in continuous-flow systems, the objective of this study was to evaluate the effect of nZVI pulses on the removal of NO from groundwater in packed soil columns and, more particularly, to elucidate whether or not biotic NO removal processes were promoted by nZVI.
View Article and Find Full Text PDFPhenolic compounds recovery by mechanical stirring extraction (MSE) was studied from orange and spinach wastes using water as a solvent. The statistical analysis showed that the highest total polyphenol content (TPC) yield was obtained using 15 min, 70 °C, 1:100 () solid/solvent ratio and pH 4 for orange; and 5 min, 50 °C, 1:50 () solid/solvent ratio and pH 6 for spinach. Under these conditions, the TPC was 1 mg gallic acid equivalent (GAE) g fresh weight (fw) and 0.
View Article and Find Full Text PDFThe use of treatments of segregated process streams as a water source, as well as technical fluid reuse as a source of value-added recovery products, is an emerging direction of resource recovery in several applications. Apart from the desired final product obtained in agro-food industries, one of the challenges is the recovery or separation of intermediate and/or secondary metabolites with high-added-value compounds (e.g.
View Article and Find Full Text PDFFruits, vegetables, spices, and herbs are a potential source of phenolic acids and polyphenols. These compounds are known as natural by-products or secondary metabolites of plants, which are present in the daily diet and provide important benefits to the human body such as antioxidant, anti-inflammatory, anticancer, anti-allergic, antihypertensive and antiviral properties, among others. Plentiful evidence has been provided on the great potential of polyphenols against different viruses that cause widespread health problems.
View Article and Find Full Text PDFThe authors would like to make the following correction to the published paper [1]: a few words should be corrected in the abstract, pages 7 and 9 [...
View Article and Find Full Text PDFThis article includes an updated review of the classification, uses and side effects of surfactants for their application in the cosmetic, personal care and pharmaceutical industries. Based on their origin and composition, surfactants can be divided into three different categories: (i) synthetic surfactants; (ii) bio-based surfactants; and (iii) microbial biosurfactants. The first group is the most widespread and cost-effective.
View Article and Find Full Text PDFIn this study, the recovery of polyphenols from olive oil mill and winery waste was investigated. The performance of ultrasound assisted extraction (UAE), microwave assisted extraction (MAE), and pressurized liquid extraction (PLE) was assessed using ethanol-water mixtures, which are compatible with food, nutraceutical, and cosmetic applications. The extraction efficiency from olive pomace and lees samples was evaluated in terms of total polyphenol content (TPC), determined by high performance liquid chromatography (HPLC) and Folin-Ciocalteu assay.
View Article and Find Full Text PDFBiosurfactants are surface-active compounds that are produced by microorganisms, which in addition to their surfactant capacity, can possess interesting antimicrobial activities that are used in their incorporation into the agrifood industry. In this work, the preservative capacity of a novel biosurfactant extract obtained from a residual stream of the corn-milling industry was evaluated against two different fungi ( and ) under different biosurfactant concentrations (0.33-0.
View Article and Find Full Text PDFBackground: This is a novel work that includes the use of a biosurfactant extract obtained from corn milling industry (named BS-CSW), with antimicrobial activity, as ingredient in formulations to treat acne vulgaris.
Methodology: With this purpose, it was established an incomplete experimental design with 3 independent variables, based on the concentration of non-nano zinc oxide (ZnO) (0-2%), BS-CSW (0-5%) and salicylic acid (0-2%).
Results: This design allowed to obtain a theoretical model that calculates the inhibitory effect on (also named ) for any formulation carried with the ingredients appointed before.
The cosmetic industry provides a wide variety of shampoos to treat dandruff, containing insoluble ingredients such as Zn pyrithione. However, the solubility of this active ingredient is quite limited in both water and oil media; thus, antidandruff formulations must include a large amount of chemically synthesized stabilizing ingredients to avoid their precipitation. In this work, the stabilization of Zn pyrithione in O/W emulsions using a biosurfactant (BS) extract and Tween 80 is studied.
View Article and Find Full Text PDFThe increase of crop production along with stricter requirements on food security have augmented the demand of new and eco-friendly bactericides. Most of the bactericides used at the moment consist of persistent organic substances, representing a risk for environmental and human health. For instance, agriculture bactericides used for crop protection includes copper-based, dithiocarbamate and amide bactericides, which are not biodegradable, resulting in the necessity of further research about the production of new active principles that attack microorganisms without producing any harmful effect on human health or environment.
View Article and Find Full Text PDFThe use of composts and vermicomposts as adsorbents is an important topic of study in the field of environmental remediation. These materials are rich in organic matter and have functional groups that can interact with organic and inorganic compounds. They also contain microorganisms that can promote biodegradation of organic substances.
View Article and Find Full Text PDFOne of the most important challenges for pharmaceutical and cosmetic industries is solubilization and preservation of their active ingredients. Therefore, most of these formulations contain irritant chemical additives to improve their shelf-life and the solubility of hydrophobic ingredients. An interesting alternative to chemical surfactants and preservatives is the use of biosurfactants; thus, their surfactant properties and composition make them more biocompatible than their chemical counterparts.
View Article and Find Full Text PDFA new and promising biosurfactant extracted from corn steep liquor has been used for the green synthesis of gold and silver nanoparticles (NPs) in a one-step procedure induced by temperature. Most of the biosurfactants proposed in the literature are produced by pathogenic microorganisms; whereas the biosurfactant used in the current work was extracted from a liquid stream, fermented spontaneously by lactic acid bacteria, which are "generally recognized as safe" (GRAS) microorganisms. The reduction of a gold precursor in the presence of a biosurfactant gives rise to a mixture of nanospheres and nanoplates with distinct optical features.
View Article and Find Full Text PDFIn this work a comparative bioadsorption study between a biocomposite consisting of hydrolysed vineyard pruning waste entrapped in calcium alginate spheres and non entrapped vineyard residue was carried out. Results have demonstrated that the biocomposite based on lignocellulose-calcium alginate spheres removed 77.3% of dyes, while non entrapped lignocellulose eliminated only removed 27.
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