[Effects of lead stress on anti-oxidative enzyme activities and isoenzymes in cucumber seedlings].

With hydroponics and polyacrylamide gel electrophoresis (PAGE), this paper studied the effects of lead stress on the activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), and their isoenzymic band numbers in cucumber seedlings. The results showed that the isoenzymic bands and expression quantity of POD decreased gradually with increasing lead concentration. The SOD activity increased with increasing lead concentration within the range of 0-500 mg Pb x L(-1), reached the maximum at 7th day, and then decreased rapidly, being lower than the control of the same period.

[Effect of ursolic acid on caspase-3 and PARP expression of human MCF-7 cells].

Objective: To study the effect of ursolic acid (UA), apentacyclic triterpene acid, on MCF-7 cell apoptosis, and probable mechanism involved by detecting the expressions of caspase-3 and poly ADP-ribose polymerase(PARP) at protein level.

Method: MCF-7 cells were cultured with different concentrations of UA. Growth inhibition of UA on MCF-7 cells was evaluated by MTT assay.

Study on the interaction of bovine serum albumin with acid cyanine 5R and its application in analysis.

A supramolecular complex of bovine serum albumin (BSA) with acid cyanine 5R (AC 5R, C.I. acid blue 113, C.

[Bidens maximowicziana's adsorption ability and remediation potential to lead in soils].

Bidens maximowicziana's adsorption ability and remediation potential to lead were studied. The results show: (1) The Bidens maximowicziana has a strong adsorption to lead, the concentration of lead in plants increased linearly with the increase of lead concentration in soil. Then maximum concentration was 1509.

[Hysteretic characteristics of atrazine desorption from fluvo-aquic soil].

Kinetic batch experiments are carried out in order to investigate the effects of a wide range of atrazine initial concentrations and consecutive desorption time on the desorption characteristics of atrazine from fluvo-aquic soil. The results reveal that, atrazine concentrations in soil solution gradually decreased with desorption time increasing, whose relationship can be expressed with empirically exponential functions. After consecutive five steps (or 5 days), when atrazine initial concentrations increased from 50 microg x L(-1) to 2000 microg x L(-1), the average desorption percentages were 23.

[Spectrum studies and application of the reaction system phenanthroline-EBT-H2O2].

A kinetic spectrophotometric method for the determination of trace phenanthroline was proposed. The method is based on the inhibition of phenanthroline to the discoloring reaction of EBT oxidized by hydrogen peroxide in the dilute acetic acid solution. At the same time, the kinetic parameters were studied in detail.

[Spectral study on the interaction mechanism between thionine and calf thymus DNA].

The interaction mechanism of thionine (TH) and calf thymus DNA (CT-DNA) was studied with UV-Vis absorption spectoscopy, fluorescence spectroscopy, circular dichroism (CD), and X-ray photoelectron spectroscopy (XPS). It was demonstrated that in the pH 7.2 phosphate buffer solution, the interaction of TH and CT-DNA is in the intercalative mode.

[Fading spectrophotometric determination of heparin with brilliant cresyl blue].

A new fading spectrophotometric method for determining trace heparin is proposed in the present paper. In pH 3.0 Britton-Robinson (B-R) buffer solution, brilliant cresyl blue can react with heparin resulting in the decrease of absorbance of BCB at 592 nm.

[Highly sensitive spectrofluorimetric determination of trace amounts of nitrite with N-(1-naphthyl) ethylenediamine].

A simple and sensitive fluorescence quenching method for the determination of trace nitrite was developed. The method is based on the reaction of NED with nitrite in acidic medium to form a new compound, which has weak fluorescence. The linear range was 1.

[Resonance-scattering spectral determination of H2O2 using rhodamine 6G association particles].

Under the conditions of 0.02 mol x L(-1) HCl-4.0 x 10(-4) mol x L(-1) KI-1.

Involvement of Bcl-2 family, cytochrome c and caspase 3 in induction of apoptosis by beauvericin in human non-small cell lung cancer cells.

Beauvericin (BEA), a cyclic hexadepsipeptide from Codyceps cicadae, possesses anti-convulsion, anti-arrhythmia, sedation, and anti-tumor activities. It has been reported that BEA induces apoptosis in several cancer cell lines. However, the molecular mechanism underlying the BEA-induced apoptotic process is not yet clearly understood.

[The in vitro cytotoxicity and in vivo toxicity of doxorubicin antiresistant stealth liposomes].

Aim: Multidrug resistance ( MDR) as a major obstacle to successful clinical cancer chemotherapy, searching a novel effective antiresistant drug would be necessary.

Methods: A novel doxorubicin anti-resistant stealth liposomes (DARSLs) was prepared by co-encapsulating doxorubicin (DOX) and verapamil (VER) into stealth liposomes with ammonium sulfate gradient remote loading approach. In vitro cytotoxity of various DOX formulations and in vivo toxicity of DARSLs were evaluated using DOX-resistant rat prostate cancer cell line (MLLB2), human uterus sarcoma cell line (MES-SA/DX5) and normal SD rats, separately.

Effects of ferulic acid on E-selectin expression in activated endothelial cell and leukocyte-endothelial cell adhesion.

Aim: To study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion.

Methods: The effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal.

[The interaction of vitamin B6 with the human serum albumin].

The Human Serum Albumin (HSA) can emit fluorescence (lamda(em) = 350 nm) under irradiation of ultraviolet light (lamda(ex) = 296 nm). After the vitamin B6 (B6) was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that ro (fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to tau(i) (fluorescence life time of HSA with B6).

In vitro efficacy of AdTRAIL gene therapy of bladder cancer is enhanced by trichostatin A-mediated restoration of CAR expression and downregulation of cFLIP and Bcl-XL.

Current therapies for bladder cancer are suboptimal and adenoviral gene therapy has been explored as an alternative treatment. In this study, we evaluated the in vitro efficacy of an adenovirus expressing TNF-related apoptosis-inducing ligand (AdTRAIL). At low concentrations of virus, T24 cells were more resistant to AdTRAIL-induced apoptosis than 5637 bladder carcinoma cells.

[Identification of antiviral activity of Toddalia asiatica against influenza type A virus].

Objective: To identify antiviral activity of Toddalia asiatica against influenza virus type A in vitro.

Method: More than two hundred Chinese medicinal herb extracts were screened for antiviral activities against influenza A/PR/8/34 (H1N1) virus using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for virus induced cytopathic effect (CPE) in a primary screening. Positive samples were picked up and were subjected to quantitative real-time polymerase chain reaction (PCR) to quantify reduction of H1N1 virus genomic RNA.

Mini-electrochemical detector for microchip electrophoresis.

This paper presents the development of a mini-electrochemical detector for microchip electrophoresis. The small size (3.6 x 5.

[Study on determination of desloratadine in human serum and its pharmacokinetics by HPLC/MS].

Objective: To study the determination of desloratadine in human serum and its pharmacokinetics in healthy volunteers.

Methods: A single oral dose of 10 mg desloratadine was given to 18 healthy volunteers. The serum concentrations of desloratadine were determined by HPLC-MS assay.

[Nanometer-size titanium dioxide separation/preconcentration and ICP-AES for the determination of Cd, Co and Zn].

The adsorption behavior of Cd, Co and Zn on nanometer-size TiQ2 was investigated. At pH 9.0, Cd, Co and Zn were retained on the nanometer-size TiO2, then eluted with 0.

[The study of in vitro refolding conditions for humanized anti-CTLA4-scFv expressed in E. coli].

The purpose of the study is to explore the in vitro refolding protocol for humanized anti-CTLA4-scFv expressed in E. coli. The inclusion bodies are denatured and then diluted or dialyzed into a refolding buffer.

[Nitrate vertical transport and simulating model in saturated soils in typical region].

By simulating soil column in laboratory, the nitrate vertical transport characteristics in farmland soil in Fengqu region of Henan Province were studied. The results show that, under saturated soil condition, the different concentrations of nitrate (100 mg x L(-1) 200mg x L(-1)) had no obvious influence on the breakthrough curves (BTCs) of nitrate vertical transport. When the soil columns were eluted by nitrate solution containing co-existing cations (K+ and Ca2+), the BTCs of nitrate vertical transport in the topsoil(0 - 30cm) of yellow fluvo-aquic soil and aeolian sandy soil were very similar.

[Bioavailability of cadmium in seawater to Paralichthys olivaceus].

With exposure experiment, this paper studied the accumulation and elimination of cadmium (Cd) in the viscera, muscle and gill of Paralichthys olivaceus, and the effects of TOC concentration in seawater on the Cd accumulation. The results showed that when the exposure concentration of Cd was 0.5 mg x L(-1), its accumulation in test tissues increased with time, and reached equilibrium on the 10th day.

[Apoptosis of human carcinoma of mouth floor KB cells and multidrug resistant KBv200 cells induced by azide methyl anthraquinone derivative].

Aim: To determine the effects of azide methyl anthraquinone derivative (AMAD) on growth inhibition and inducing apoptosis of multidrug resistant (MDR) KBv200 cells and parental drug-sensitive KB cells.

Methods: Cytotoxicity was determined by tetrazolium (MTF) assay. Reactive oxygen species (ROS) levels and mitochondrial membrane potential (deltapsi(m)) in cells were labeled with DCFH-DA and DiOC6 and tested by flow cytometry.

[Effects of acid rain on nitrogen content in the water body of Wenzhou Sanyang wetland].

In order to understand the effects of acid rain on the nitrogen (N) content in the water body of Wenzhou Sanyang wetland, this paper measured the concentrations of different N forms in the wetland, of which, NH4+-N was 2.90-10.75 mg x L(-1), average in 5.

[Speciation analysis of selenium by flow-injection catalytic spectrophotometry].

A new flow-injection catalytic spectrophotometric method was proposed for the determination of Se(IV) based on its catalytic effect on the reduction reaction of azure I by Na2S in pH 7.0 citric-acid sodium-hydrogen-phosphate buffer solution. Some different schematic diagrams of FIA were compared.