Hematopoietic malignancies associated with increased Stat5 and Bcl-x(L) expressions in Ink4a/Arf-deficient mice.

The INK4a/ARF locus, which encodes the two distinct proteins p16(INK4a) and p14(ARF), is frequently altered in various hematological malignancies as well as in other types of cancers in humans. In this study, we surveyed tumors that had spontaneously developed in Ink4a/Arf-deficient mice with an inbred FVB/NJ genetic background. We found that an Ink4a/Arf-deficiency exerted more severe effects on the induction of hematopoietic malignancies in mice with an inbred FVB/NJ genetic background than in mice with a mixed genetic background.

[Determination of trace silver by flame atomic absorption spectrometry using chitosan enriching method].

A method for the determination of trace silver by flame atomic absorption spectrometry using chitosan enriching method has been investigated. The conditions that influence the adsorption of silver by chitosan were studied. The optimal flow rate of the sample solution was 1 mL x min(-1), and the optimal sorption (96.

[Determination of Levofloxacin by chemiluminescence method].

A novel chemiluminescence method for the determination of Levofloxacin is based on its enhancement effect on thereaction of luminol with H2O2. The calibration graph is linear over the range of 5.53 x 10(-11)-2.

[Determination of microamounts of palladium by triple-wavelength overlapping spectrophotometry].

The method for the determination of microamounts of palladium with 2-(3,5-dichlolor-2-pyridylazo)-5-dimethylaminoaniline (3,5-diCl-PADMA) by triple-wavelength overlapping spectrophotometry has been developed. It's shown that in the medium of 0.02-1.

[Determination of trace germanium in coal by spectrophotometry with 2,4-dichlorophenylfluorone].

A method for the determination of germanium in coal samples by spectrophotometry has been developed. The color reaction of germanium with 2,4-dichlorophenylfluorone (2,4-DCIPF) in the presence of cetyltrimethylammonium bromide (CTMAB) has been studied. The results show that Ge(IV) reacts on 2,4-DCIPF and CTMAB to form a multicomponent complex in a 1.

[Soil biochemical characters of Pinus koraiensis and Pinus sylvestriformis plantations under different elevated CO2 concentration].

The aim of this study was to determine the dynamics of soil microbial biomass C, cellulose decomposition rate and catalase activity in Pinus koraiensis and Pinus sylvestriformis plantations exposed to elevated CO2 (700 mg x L(-1) and 500 mg x L(-1)) during the growth season. The five years results indicated that in Autumn, the soil microbial biomass C of Pinus koraiensis and Pinus sylvestriformis plantations was in the order of ambient CO2-chamber > 500 mg x L(-1) CO2-chamber > 700 mg x L(-1) CO2-chamber. The monthly dynamics of cellulose decomposition rate in 5 and 10 cm depth soils showed that for both Pinus sylvestriformis and Pinus koraiensis plantations, it was always higher under 500 mg x L(-1) CO2 than under 700 mg x L(-1) CO2 in growth season.

Activity of green tea polyphenol epigallocatechin-3-gallate against ovarian carcinoma cell lines.

Purpose: A constituent of green tea, (-)-epigallocatechin-3-gallate (EGCG), is known to possess anti-cancer properties. In this study, the time-course of the anticancer effects of EGCG on human ovarian cancer cells were investigated to provide insights into the molecular-level understanding of the growth suppression mechanism involved in EGCG-mediated apoptosis and cell cycle arrest.

Materials And Methods: Three human ovarian cancer cell lines (p53 negative, SKOV-3 cells; mutant type p53, OVCAR-3 cells; and wild type p53, PA-1 cells) were used.

Two-promoter vector is highly efficient for overproduction of protein complexes.

The use of bicistronic vectors, which contain two target genes under one promoter, has been the most common practice for the heterologous production of binary protein complexes. The major problem of this method is the much lower expression of the second gene compared with that of the first gene next to the promoter. We tested a simple idea of whether inclusion of an additional promoter in front of the second gene may remove the problem.

Temporospatial sequence of cellular events associated with etoposide-induced neuronal cell death: role of antiapoptotic protein Bcl-X(L).

Etoposide-induced death comprises such nuclear events as the formation of topoisomerase II-DNA cleavable complex and cytosolic events including caspase activation. By first establishing the temporospatial death sequence triggered by etoposide in a neuronal cell line, MN9D overexpressing Bcl-X(L) (MN9D/Bcl-X(L)) or control vector (MN9D/Neo), we examined whether formation of this complex is primarily responsible for cell death and at which strategic points and how Bcl-X(L) blocks etoposide-induced neuronal death. Etoposide induced death that was dependent on caspase, cycloheximide, and calpain in MN9D/Neo cells.

Odontogenic ghost cell carcinoma: a case report with reference to the relation between apoptosis and ghost cells.

The neoplastic variant of calcifying odontogenic cyst has various designations, and its malignant counterpart has been reported as aggressive epithelial odontogenic ghost cell tumor or odontogenic ghost cell carcinoma. We present a case of odontogenic ghost cell carcinoma with reference to the relation between the ghost cells and apoptosis. A 33-year-old man complained of a mandibular mass.

Adenosine-supplemented blood cardioplegia attenuates postischemic dysfunction after severe regional ischemia.

Background: Various studies have reported that the administration of adenosine (ADO) in cardioplegia reduces myocardial ischemic injury, but this timing may not utilize ADO's potential against myocardial reperfusion injury. This study tested the hypothesis that ADO-supplemented blood cardioplegia (BCP) or ADO administered during reperfusion reduces postischemic dysfunction after severe regional ischemia.

Methods And Results: After 75 minutes of left anterior descending coronary artery occlusion, total cardiopulmonary bypass was initiated; cold (4 degrees C) antegrade BCP (8:1 blood:crystalloid) was delivered every 20 minutes for the first 3 doses, and 27 degrees C BCP was delivered for the terminal infusion.

Curcumin causes the growth arrest and apoptosis of B cell lymphoma by downregulation of egr-1, c-myc, bcl-XL, NF-kappa B, and p53.

It has been well known that curcumin is a powerful inhibitor of proliferation of several tumor cells. However, the molecular basis of the anti-proliferative effect of curcumin has not been investigated in detail. In this paper, we present evidence to show that curcumin inhibited proliferation of a variety of B lymphoma cells.