Antigen-independent single-cell circulating tumor cell detection using deep-learning-assisted biolasers.

Circulating tumor cells (CTCs) in the bloodstream are important biomarkers for clinical prognosis of cancers. Current CTC identification methods are based on immuno-labeling, which depends on the differential expression of specific antigens between the cancer cells and white blood cells. Here we present an antigen-independent CTC detection method utilizing a deep-learning-assisted single-cell biolaser.

The complete chloroplast genome sequence of (Primulaceae) and its phylogeny.

W.B Ju, B. Xu & X.

In Situ Ions Induced Formation of KF-Rich SEI Layers toward Ultrastable Life of Potassium-Ion Batteries.

Engineering F-rich solid electrolyte interphase (SEI) layers is regarded as an effective strategy to enable the long-term cycling stability of potassium-ion batteries (KIBs). However, in the conventional KPF carbonate electrolytes, it is challenging to form F-containing SEI layers due to the inability of KPF to decompose into KF. Herein, AlCl is employed as a novel additive to change the chemical environment of the KPF carbonate electrolyte.

Organic Molecules Mimic Alkali Metals Enabling Spontaneous Harpoon Reactions with Halogens.

The harpoon mechanism has been a milestone in molecular reaction dynamics. Until now, the entity from which electron harpooning occurs has been either alkali metal atoms or non-metallic analogs in their excited states. In this work, we demonstrate that a common organic molecule, octamethylcalix[4] pyrrole (omC4P), behaves just like alkali metal atoms, enabling the formation of charge-separated ionic bonding complexes with halogens omC4P ⋅ X (X=F-I, SCN) via the harpoon mechanism.

Anion-Activated Bases and Nucleophiles Characterized by Photoelectron Spectroscopy.

Negative ion photoelectron spectra at 20 K along with ab initio [CCSD(T)] and M06-2X density functional theory calculations are reported for a series of six basic and nucleophilic pyridine derivatives with an anionic substituent [i.e., 3- and 4-PyrBX, where X = F, 4--BuCH, 4-MeOCH, and 3,5-(MeO)CH].

Glycolysis Promotes Angiotensin II-Induced Aortic Remodeling Through Regulating Endothelial-to-Mesenchymal Transition via the Corepressor C-Terminal Binding Protein 1.

Background: Endothelial dysfunction plays a crucial role in aortic remodeling. Aerobic glycolysis and endothelial-to-mesenchymal transition (EndoMT) have, respectively, been suggested to contribute to endothelial dysfunction in many cardiovascular diseases. Here, we tested the hypothesis that glycolytic reprogramming is critical for EndoMT induction in aortic remodeling through an epigenetic mechanism mediated by a transcriptional corepressor CtBP1 (C-terminal binding protein 1), a sensor of glycolysis-derived NADH.

The Effect of Using Premedication of Simethicone/Pronase With or Without Postural Change on Visualization of the Mucosa Before Endoscopy: A Prospective, Double Blinded, Randomized Controlled Trial.

Introduction: To investigate the efficacy and safety of preprocedural simethicone (S) and pronase (P) for optimal mucosal visualization during esophagogastroduodenoscopy with sedation. The effect of postural change combined with premedication on mucosal visibility was also examined.

Methods: The study randomized 496 patients into 8 groups based on the type of premedication provided and whether a postural change occurred.

Theoretical investigation on the elusive structure-activity relationship of bioinspired high-valence nickel-halogen complexes in oxidative fluorination reactions.

Very recently, bioinspired high-valence metal-halogen complexes have been proven to be competent oxidants in the C-H bond activation and heteroatom dihalogenation reactions. However, the structure-activity relationship of such active species and the reaction mechanisms of oxidations mediated by these oxidants are still elusive. In this study, density functional theory (DFT) calculations were performed to systematically study the oxidizing ability of the high-valence Ni-X (X = F and Cl) complexes EtN[Ni(Cl/F)(L)], (1, Et = ethyl, L = '-(2,6-dimethylphenyl)-2,6-pyridinedicarboxamide), such as the reaction mechanism of fluorination of 1,4-cyclohexadiene (CHD) by 1 in the presence of AgF and the reaction mechanism of difluorination of triphenyl phosphine (PPh) by 1.

Comparison of σ/ π-hole aerogen-bonding interactions based on CH···NgOX (Ng = Kr, Xe; X = F, Cl, Br) complexes.

The geometric structure, energy properties, and electronic properties of the aerogen-bonding interaction formed by CH and NgOX (Ng = Kr, Xe; X = F, Cl, Br) have been studied at the B2PLYP-D3(BJ)/ aug-cc-pVTZ (PP) level. Two kinds of aerogen-bonding interactions were observed among the title systems: the σ-hole and the π-hole complexes. The σ-hole aerogen-bonding complex has a binding energy in the range of - 6.

A highly immunogenic live-attenuated vaccine candidate prevents SARS-CoV-2 infection and transmission in hamsters.

The highly pathogenic and readily transmissible SARS-CoV-2 has caused a global coronavirus pandemic, urgently requiring effective countermeasures against its rapid expansion. All available vaccine platforms are being used to generate safe and effective COVID-19 vaccines. Here, we generated a live-attenuated candidate vaccine strain by serial passaging of a SARS-CoV-2 clinical isolate in Vero cells.

A tumor-specific pro-IL-12 activates preexisting cytotoxic T cells to control established tumors.

It is a challenge to effectively reactivate preexisting tumor-infiltrating lymphocytes (TILs) without causing severe toxicity. Interleukin-12 (IL-12) can potently activate lymphocytes, but its clinical use is limited by its short half-life and dose-related toxicity. In this study, we developed a tumor-conditional IL-12 (pro-IL-12), which masked IL-12 with selective extracellular receptor–binding domains of the IL-12 receptor while preferentially and persistently activating TILs after being unmasked by matrix metalloproteinases expressed by tumors.

Electronic Structure and Spectroscopic Properties of Group-7 Tri-Oxo-Halides MOX (M = Mn-Bh, X = F-Ts).

The 24 trioxide halide molecules MOX of the manganese group (M = Mn-Bh; X = F-Ts), which are iso-valence-electronic with the famous MnO ion, have been quantum-chemically investigated by quasi-relativistic density-functional and ab initio correlated approaches. Geometric and electronic structures, valence and oxidation numbers, vibrational and electronic spectral properties, energetic stabilities of the monomers in the gas phase, and the decay mode of MnOF have been investigated. The light Mn-3d species are most strongly electron-correlated, indicating that the concept of a closed-shell Lewis-type single-configurational structure [Mn(d) O(p) F(p)] reaches its limits.

FTO (Fat-Mass and Obesity-Associated Protein) Participates in Hemorrhage-Induced Thalamic Pain by Stabilizing Toll-Like Receptor 4 Expression in Thalamic Neurons.

Background And Purpose: Hemorrhage-caused gene changes in the thalamus likely contribute to thalamic pain genesis. RNA N6-methyladenosine modification is an additional layer of gene regulation. Whether FTO (fat-mass and obesity-associated protein), an N6-methyladenosine demethylase, participates in hemorrhage-induced thalamic pain is unknown.

Gaseous cyclodextrin--dodecaborate complexes χCD·BX (χ = α, β, and γ; X = F, Cl, Br, and I): electronic structures and intramolecular interactions.

A fundamental understanding of cyclodextrin-closo-dodecaborate inclusion complexes is of great interest in supramolecular chemistry. Herein, we report a systematic investigation on the electronic structures and intramolecular interactions of perhalogenated closo-dodecaborate dianions B12X122- (X = F, Cl, Br and I) binding to α-, β-, and γ-cyclodextrins (CDs) in the gas phase using combined negative ion photoelectron spectroscopy (NIPES) and density functional theory (DFT) calculations. The vertical detachment energy (VDE) of each complex and electronic stabilization of each dianion due to the CD binding (ΔVDE, relative to the corresponding isolated B12X122-) are determined from the experiments along α-, β- and γ-CD in the form of VDE (ΔVDE): 4.

Efficacy and Safety of Thermal Ablation for Treatment of Solitary T1N0M0 Papillary Thyroid Carcinoma: A Multicenter Retrospective Study.

Background Microwave ablation (MWA) and radiofrequency ablation (RFA) have recently attracted interest as minimally invasive treatment modalities for papillary thyroid carcinoma (PTC). However, the ablation outcomes of T1N0M0 PTC are not well characterized. Purpose To evaluate the efficacy and safety of thermal ablation (MWA or RFA) of solitary T1N0M0 PTC in patients who were ineligible for (due to presence of comorbid cardiovascular disease, renal failure, other malignancy, etc) or who refused surgery.

Suppressing the Voltage Decay Based on a Distinct Stacking Sequence of Oxygen Atoms for Li-Rich Cathode Materials.

Li-rich cathode materials possess a much higher theoretical energy density than all intercalated cathode materials currently reported and thus are considered as the most promising candidate for next-generation high-energy density Li-ion batteries. However, the rapid voltage decay and the irreversible phase transition of O3-type Li-rich cathode materials often lessen their actual energy density and limit their practical applications, and thus, effectively suppressing the voltage decay of Li-rich cathodes becomes the hotspot of the current research. Herein, the F-doped O2-type Li-rich cathode materials LiMnNiCoOF (F-O2-LRO) are designed and prepared based on the P2-type sodium-ion cathode materials NaLi(MnNiCo)O (Na-LRO) by ion exchange.

Acute Kidney Injury Sensitizes the Brain Vasculature to Ang II (Angiotensin II) Constriction via FGFBP1 (Fibroblast Growth Factor Binding Protein 1).

Acute kidney injury (AKI) causes multiple organ dysfunction. Here, we identify a possible mechanism that can drive brain vessel injury after AKI. We induced 30-minute bilateral renal ischemia-reperfusion injury in C57Bl/6 mice and isolated brain microvessels and macrovessels 24 hours or 1 week later to test their responses to vasoconstrictors and found that after AKI brain vessels were sensitized to Ang II (angiotensin II).

Decomposition of highly persistent perfluorooctanoic acid by hollow Bi/BiOIF: Synergistic effects of surface plasmon resonance and modified band structures.

Perfluorooctanoic acid (PFOA) is highly stable due to the strong CF bond and extremely difficult to be removed by conventional photocatalysts. In this study, Bi doped BiOIF solid solutions with hollow microsphere structure were prepared through a facile one-step hydrothermal method. Compared with pure BiOI and BiOF, the band gap of the Bi/BiOIF solid solutions was significantly reduced, thus promoting the visible light absorbance.

Spectroscopic evidence for intact carbonic acid stabilized by halide anions in the gas phase.

This work shows elusive carbonic acid being effectively stabilized in the gas phase by interacting with halide anions X (X = F, Cl, Br, and I). The formed HCO·X complexes, characterized by negative ion photoelectron spectroscopy and ab initio calculations, all contain intact trans-trans carbonic acid binding onto the respective halide via two identical strong ionic O-HX hydrogen bonds. For X = Cl, Br, and I, the complex spectra exhibit the corresponding X signature by simply shifting to the higher binding energy side, while an extremely 2 eV wide broader band is observed for X = F.

Homeostatic and early-recruited CD101 eosinophils suppress endotoxin-induced acute lung injury.

Introduction: Acute lung injury (ALI) is a fatal but undertreated condition with severe neutrophilic inflammation, although little is known about the functions of eosinophils in the pathogenesis of ALI. Our objectives were to investigate the roles and molecular mechanisms of eosinophils in ALI.

Methods: Pulmonary eosinophils were identified by flow cytometry.

Using Artificial Intelligence to Detect COVID-19 and Community-acquired Pneumonia Based on Pulmonary CT: Evaluation of the Diagnostic Accuracy.

Background Coronavirus disease 2019 (COVID-19) has widely spread all over the world since the beginning of 2020. It is desirable to develop automatic and accurate detection of COVID-19 using chest CT. Purpose To develop a fully automatic framework to detect COVID-19 using chest CT and evaluate its performance.

Probing Orientation-Specific Charge-Dipole Interactions between Hexafluoroisopropanol and Halides: A Joint Photoelectron Spectroscopy and Theoretical Study.

The interactions between hexafluoroisopropanol (HFIP) and halogen anions X (F, Cl, Br, and I) have been investigated using negative ion photoelectron (NIPE) spectroscopy and ab initio calculations. The measured NIPE spectrum of each [HFIP·X] (X = Cl, Br, and I) complex shows a pattern identical to the corresponding X by shifting to the high electron binding energy side, indicative of the formation of the [HFIP···X] structure in which X interacts with HFIP via charge-dipole interactions. However, the spectrum of [HFIP·F] appears completely different from that of F and is more similar to the spectrum of the deprotonated HFIP anion (HFIP).

Association of Lipids With Ischemic and Hemorrhagic Stroke: A Prospective Cohort Study Among 267 500 Chinese.

Background and Purpose- Previous results on the association between lipids and stroke were controversial. We investigated the association of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C ), high-density lipoprotein cholesterol (HDL-C), and triglyceride with stroke. Methods- Six cohort studies in China with 267 500 participants were included.