Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers.

Objectives: The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.

Background: Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level.

Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography.

A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative.

Effects of smoking on fatty acid composition of phospholipid sperm membrane and the malondialdehyde levels in human seminal plasma.

The aim of this study was to investigate fatty acids composition of sperm phospholipids, level of lipoperoxidation represented by malondialdehyde and to examine differences between recent smokers and nonsmokers. The levels of malondialdehyde were in the group of all patients 1.51 ± 0.

A monitoring of allantoin, uric acid, and malondialdehyde levels in plasma and erythrocytes after ten minutes of running activity.

Uric acid is the final product of human purine metabolism. It was pointed out that this compound acts as an antioxidant and is able to react with reactive oxygen species forming allantoin. Therefore, the measurement of allantoin levels may be used for the determination of oxidative stress in humans.

[Role of fatty acids in sperm membrane].

One of causes of male infertility is reduced sperm motility. Reactive oxygen species (ROS) play significant role for physiological sperm function. Oxidative stress occurs when the production of potentially destructive ROS exceeds the natural antioxidant defences, resulting in cell damage.