Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization.

Background: Polydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the potential molecular mechanisms of PD in osteoarthritis have not been clearly elucidated.

Anisotropic Microparticles with a Controllable Structure via Soap-Free Seeded Emulsion Polymerization.

Anisotropic particles have a wide range of applications in materials science such as emulsion stabilization, oil-water separation, and catalysis due to their asymmetric structure and properties. Nevertheless, designing and synthesizing large quantities of anisotropic particles with controlled morphologies continue to present considerable challenges. In this study, we successfully synthesized anisotropic microspheres using a soap-free seed emulsion polymerization method.

Extracellular vesicles promote the infection and pathogenicity of Japanese encephalitis virus.

Japanese encephalitis virus (JEV) is a neurotropic zoonotic pathogen that poses a serious threat to public health. Currently, there is no specific therapeutic agent available for JEV infection, primarily due to the complexity of its infection mechanism and pathogenesis. Extracellular vesicles (EVs) have been known to play an important role in viral infection, but their specific functions in JEV infection remain unknown.

ZFP36L2 Is a Potential Prognostic Marker of IL1β Osteosarcoma.

Osteosarcoma stands as the predominant bone malignancy afflicting children and young adults. Despite strides in treatment, the enduring reality is that the long-term survival rates for patients grappling with recurrences and metastases linger at a mere 30%. This underscores the pressing demand for novel prognostic markers and therapeutic avenues to improve outcomes and offer hope to those battling this formidable disease.

Production of virus-like particles of FMDV by 3C protease cleaving precursor polyprotein P1 in vitro.

Nonstructural protein 3C, a master protease of Picornaviridae, plays a critical role in viral replication by directly cleaving the viral precursor polyprotein to form the viral capsid protein and antagonizing the host antiviral response. Additionally, 3C protease, as a tool enzyme, is involved in regulating polyprotein expression. Here, the 3C mutant gene (3Cm), fused with a small ubiquitin-like modifier (SUMO) tag at the N-terminal and featuring a mutation at position 127, was inserted into the cold-shock plasmid pCold of Escherichia coli for expression.