The Role and Mechanism of CREBH Regulating SIRT3 in Metabolic Associated Fatty Liver Disease.

Aims: To investigate the effect of cAMP response element-binding protein H (CREBH) on metabolic associated fatty liver disease by regulating sirtuin 3 (SIRT3).

Main Methods: Two mouse models of fatty liver induced by a methionine-choline deficient (MCD) diet and a high-fat (HF) diet and an in vitro model of palmitic acid (PA) induced lipid-overloaded hepatocytes were constructed to detect the expression of CREBH, SIRT3, total acetylation, and downstream protein interactions and lipid metabolism phenotype, which were further validated in CREBH mice and lentivirus-overexpressing CREBH hepatocytes.

Key Findings: In fatty liver and lipid overload models, the expressions of CREBH and SIRT3 were down-regulated and their expression was positively correlated, accompanied by an increase in the level of total protein acetylation.

Complex optical field reconstruction by separating the Fourier spectrum.

A complex optical field in a Fourier plane is experimentally recovered by separating the real and imaginary parts of the Fourier spectrum in real time without an additional inverted object function U(-x, -y), and the signs of both parts are derived by differentiating the real spectrum.