Different Internal Fixation Methods for Sanders Type II and III Calcaneal Fractures: A 5-Year Retrospective Study and Finite Element Analysis.

Objective: Soft tissue defects and postoperative wound healing complications related to calcaneus fractures may result in significant morbidity. The aim of this study was to investigate whether percutaneous minimally invasive screw internal fixation (PMISIF) can change this situation in the treatment of calcaneal fractures, and aimed to explore the mechanical effects of different internal fixation methods on Sanders type III calcaneal fractures through finite element analysis.

Methods: This retrospective analysis focused on 83 patients with Sanders II and III calcaneal fractures from March 2017 to March 2022.

BMP6, a potential biomarker of inflammatory fibrosis and promising protective factor for dilated cardiomyopathy.

Background: Dilated cardiomyopathy (DCM) stands as one of the most prevalent and severe causes of heart failure. Inflammation plays a pivotal role throughout the progression of DCM to heart failure, while age acts as a natural predisposing factor for all cardiovascular diseases. These two factors often interact, contributing to cardiac fibrosis, which is both a common manifestation and a pathogenic driver of adverse remodeling in DCM-induced heart failure.

Air nanobubble simultaneously enhances hydrolysis and methane yield of sludge temperature phased-anaerobic digestion.

Nanobubble water (NBW) or temperature-phased anaerobic digestion assisted by microbial electrolysis cell (MEC-TPAD) can promote sludge hydrolysis and methanogenesis. However, the role of the combined application of NBW and MEC-TPAD in terms of anaerobic performance and related microbial properties remains unclear. This study investigated the impact of Air-NBW on hydrolysis and methanogenesis of dewatered sludge MEC-TPAD.

The dCas9/crRNA linked immunological assay (dCLISA) for sensitive, accurate, and facile drug resistance gene analysis.

The rapid and reliable diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is essential for preventing the spread of MRSA infections and guiding therapeutic strategies. However, there is still a huge challenge in further simplifying MRSA detection procedures and improving detection selectivity to reduce false-positive results. In this study, we developed a derivative CRISPR-associated protein 9/CRISPR-derived RNA Linked Immunological Assay (dCLISA) for the sensitive and specific detection of MRSA.

YTHDF2-mediated mA modification of ONECUT2 promotes stemness and oxaliplatin resistance in gastric cancer through transcriptionally activating TFPI.

Aims: Chemoresistance results in poor outcomes of patients with gastric cancer (GC). This study aims to identify oxaliplatin resistance-related cell subpopulations in the tumor microenvironment (TME) and decipher the involved molecular mechanisms.

Methods: Through single-cell RNA sequencing, a unique ONECUT2TFPI GC cell subset was identified in the oxaliplatin-resistant TME.