Silver-coated PMMA nanoparticles-on-a-mirror substrates as high-performance SERS sensors for detecting infinitesimal molecules.

Surface-enhanced Raman scattering (SERS) technology has attracted more and more attention due to its high sensitivity, low water interference, and quick measurement. Constructing high-performance SERS substrates with high sensitivity, uniformity and reproducibility is of great importance to put the SERS technology into practical application. In this paper, we report a simple fabrication process to construct dense silver-coated PMMA nanoparticles-on-a-mirror SRES substrates.

Alleviating batch effects in cell type deconvolution with SCCAF-D.

Cell type deconvolution methods can impute cell proportions from bulk transcriptomics data, revealing changes in disease progression or organ development. But benchmarking studies often use simulated bulk data from the same source as the reference, which limits its application scenarios. This study examines batch effects in deconvolution and introduces SCCAF-D, a computational workflow that ensures a Pearson Correlation Coefficient above 0.

Sub-millimeter fiberscopic robot with integrated maneuvering, imaging, and biomedical operation abilities.

Small-scale continuum robots hold promise for interventional diagnosis and treatment, yet existing models struggle to achieve small size, precise steering, and visualized functional treatment simultaneously, termed an "impossible trinity". This study introduces an optical fiber-based continuum robot integrated imaging, high-precision motion, and multifunctional operation abilities at submillimeter-scale. With a slim profile of 0.

Light-dependent modulation of protein localization and function in living bacteria cells.

Most bacteria lack membrane-enclosed organelles and rely on macromolecular scaffolds at different subcellular locations to recruit proteins for specific functions. Here, we demonstrate that the optogenetic CRY2-CIB1 system from Arabidopsis thaliana can be used to rapidly direct proteins to different subcellular locations with varying efficiencies in live Escherichia coli cells, including the nucleoid, the cell pole, the membrane, and the midcell division plane. Such light-induced re-localization can be used to rapidly inhibit cytokinesis in actively dividing E.

Accurate stacking engineering of MOF nanosheets as membranes for precise H sieving.

Two-dimensional (2D) metal-organic framework (MOF) nanosheet membranes hold promise for exact molecular transfer due to their structural diversity and well-defined in-plane nanochannels. However, achieving precise regulation of stacking modes between neighboring nanosheets in membrane applications and understanding its influence on separation performance remains unrevealed and challenging. Here, we propose a strategy for accurately controlling the stacking modes of MOF nanosheets via linker polarity regulation.