Characterization of Key Lipid Components in the Cell Membrane of Freeze-Drying Resistant Strains Using Nontargeted Lipidomics.

Lactic acid bacteria (LAB) are usually freeze-dried into powder for transportation and storage, with the bacterial membrane playing a crucial role in this process. However, different strains exhibit different levels of freeze-drying resistance in their cell membranes. In this study, () strains 1F20, K56, and J5, demonstrating survival rates of 59.

The impact of neoadjuvant therapy on the prognostic value of preoperative neutrophil-to-lymphocyte ratio for colorectal liver metastases: a multi-center cohort study.

Background: Neutrophil-to-lymphocyte ratio (NLR) is a promising prognostic marker for patients undergoing hepatectomy for colorectal liver metastases (CRLM). However, its prognostic value in patients receiving neoadjuvant therapy (NAT) has not been sufficiently addressed.

Methods: From 2013 to 2023, a cohort of 692 patients with CRLM receiving hepatectomy were enrolled in five centers.

Biomarkers.

Background: The development of reliable blood biomarkers for neurodegenerative diseases (NDDs) has been hindered by the lack of tools with sufficient sensitivity to detect low concentrations of brain-derived proteins in plasma or serum in a highly multiplexed manner. NULISA™ (NUcleic acid-Linked Immuno-Sandwich Assay) has emerged as a promising solution, with attomolar sensitivity and capable of high multiplexing in a fully automated system. In this study, we introduce NULISA CNS Disease Panel 120, a 120-plex NULISAseq assay for profiling key hallmarks of NDDs in both blood and cerebrospinal fluid (CSF).

Biomarkers.

Background: Growing evidence indicates that people with neurodegenerative diseases have altered metabolic status, but the association between metabolic age (MetAge), as assessed by circulating plasma metabolomics, and dementia remains unclear. We aimed to investigate the association between MetAge and risk of dementia and to explore whether genetic background plays a role in these associations.

Method: From the UK Biobank, 153,436 dementia-free adults aged ≥55 (mean age 62.

Biomarkers.

Background: The present study aimed to establish a panel of plasma proteins that are associated with cognitive performance and amyloid-β burden and establish their utility for distinguishing early Alzheimer's disease (AD) from healthy controls.

Method: Plasma from fifty patients with early AD (all amyloid-positive) and forty-nine healthy controls (HCs) were assayed using quantitative mass spectrometry. The weighted correlation network analysis (WGCNA) was used to explore the association between plasma protein levels and cognitive performance and filter out the high-performance plasma proteomic biomarker candidates.