Publications by authors named "X A Casabiell"
Background: A therapeutic approach for the treatment of glucocorticoid-induced skeletal muscle atrophy should be based on the knowledge of the molecular mechanisms determining the unbalance between anabolic and catabolic processes and how to re-establish this balance. Here, we investigated whether the obestatin/GPR39 system, an autocrine signalling system acting on myogenesis and with anabolic effects on the skeletal muscle, could protect against chronic glucocorticoid-induced muscle atrophy.
Methods: In this study, we used an in vivo model of muscle atrophy induced by the synthetic glucocorticoid dexamethasone to examine the liaison molecules that define the interaction between the glucocorticoid receptor and the obestatin/GPR39 systems.
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View Article and Find Full Text PDFBackground: This study was performed to test the therapeutic potential of obestatin, an autocrine anabolic factor regulating skeletal muscle repair, to ameliorate the Duchenne muscular dystrophy (DMD) phenotype.
Methods And Results: Using a multidisciplinary approach, we characterized the ageing-related preproghrelin/GPR39 expression patterns in tibialis anterior (TA) muscles of 4-, 8-, and 18-week-old mdx mice (n = 3/group) and established the effects of obestatin administration at this level in 8-week-old mdx mice (n = 5/group). The findings were extended to in vitro effects on human immortalized DMD myotubes.
Article Synopsis
- The study explores how the obestatin/GPR39 signaling system may protect against muscle atrophy induced by glucocorticoids, which are linked to poor patient outcomes.
- Researchers used mouse C2C12 myotube cultures and human KM155C25 myotubes to examine the effects of this system on muscle degradation and found notable differences between mouse and human responses.
- The findings highlight the importance of the obestatin/GPR39 system in regulating various pathways that control protein degradation during muscle-wasting conditions.
Article Synopsis
- Obestatin/GPR39 signaling is crucial in promoting skeletal muscle growth and repair through both G-protein-dependent and -independent pathways, involving various proteins.
- This signaling helps determine the oxidative fiber type in muscles, impacting their formation and metabolic properties.
- Increased activity of Mef2 and PGC-1α from obestatin signaling enhances muscle oxidative capacity and myofiber growth, indicating its role in muscle fiber-type programming.