Turtle Girdles: Comparing the Relationships Between Environment and Behavior on Forelimb Function in Loggerhead Sea Turtles (Caretta caretta) and River Cooters (Pseudemys concinna).

Locomotion in water and on land impose dramatically different demands, yet many animals successfully move in both environments. Most turtle species perform both aquatic and terrestrial locomotion but vary in how they use their limbs. Freshwater turtles use anteroposterior movements of the limbs during walking and swimming with contralateral fore- and hindlimbs moving in synchrony.

Intraosseous contrast administration for coelomic computed tomography angiography in green iguanas (Iguana iguana): Preliminary findings show promise as an alternative to the intravenous route.

Computed tomography is commonly used to evaluate the reptile coelom. One challenge in the acquisition of contrast-enhanced CT studies in reptiles is the difficulty in placing intravenous catheters due to the poor conspicuity of peripheral vessels. To overcome these limitations, intraosseous catheterization of the long bones (humerus, femur, and tibia) can be used.

Correlations between local geoclimatic variables and hatchling body size in the sea turtles Caretta caretta and Chelonia mydas.

It has been widely demonstrated that air and sand temperatures influence the anatomy of sea turtle hatchlings. We examined the impact of precipitation during the nesting season on the hatchling body size of loggerhead and green turtles from 37 beaches worldwide. Longitudinal data collected between 2012 and 2018 from Florida (US) and from a sample on Bõa Vista Island (Cabo Verde) carried out in 2019 showed that loggerhead body size at hatching was negatively correlated with precipitation, while precipitation was not correlated with hatchling body size in green turtles.

Baseline Skin Microbiota of the Leatherback Sea Turtle.

The integumentary system of the leatherback sea turtle () is the most visible and defining difference of the species, with its smooth and waxy carapace and finely scaled skin, distinguishing it from the other six sea turtle species. The skin is the body's largest organ and serves as a primary defense against the outside world and is thus essential to health. To date, we have begun to understand that the microorganisms located on the skin aid in these functions.

Novel Microsatellite Tags Hold Promise for Illuminating the Lost Years in Four Sea Turtle Species.

After hatching, sea turtles leave the nest and disperse into the ocean. Many years later, they return to their natal coastlines. The period between their leaving and their returning to natal areas, known as the "Lost Years", is poorly understood.

Aneurysm Associated with Vascular Wall Degeneration in Bearded Dragons ().

This study describes the clinical, gross, and histologic findings in 17 cases of aneurysms in bearded dragons (). The clinical presentation ranged from incidental to sudden and unexpected death. The affected vasculature was predominantly arterial; however, based on the topographical locations of the lesions, gross structure, and drainage, some veins were likely involved.

Adaptation of sea turtles to climate warming: Will phenological responses be sufficient to counteract changes in reproductive output?

Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.

3D visualization processes for recreating and studying organismal form.

The study of biological form is a vital goal of evolutionary biology and functional morphology. We review an emerging set of methods that allow scientists to create and study accurate 3D models of living organisms and animate those models for biomechanical and fluid dynamic analyses. The methods for creating such models include 3D photogrammetry, laser and CT scanning, and 3D software.

Comparative and Functional Anatomy of the Ectothermic Sauropsid Heart.

The heart development, form, and functional specializations of chelonians, squamates, crocodilians, and birds characterize how diverse structure and specializations arise from similar foundations. This review aims to summarize the morphologic diversity of sauropsid hearts and present it in an integrative functional and phylogenetic context. Besides the detailed morphologic descriptions, the integrative view of function, evolution, and development will aid understanding of the surprising diversity of sauropsid hearts.

Two heads are not always better than one: Craniofacial and axial bifurcation in cheloniid embryos and hatchlings (Chelonia mydas and Caretta caretta).

Morphological data on craniofacial and axial bifurcation in sea turtles is not well documented in the literature. Here, we use micro-computed tomography (μ-CT) imaging to describe the body, skull, and vertebral morphology in axially-bifurcated cheloniid sea turtle embryos and hatchlings (Chelonia mydas and Caretta caretta) from south Florida beaches. We describe three types of craniofacial and axial bifurcations: bifacial, bicephalic, and bicephalic with biaxial duplication ranging from facial bones to the sacrum.

First Atlantic satellite tracks of 'lost years' green turtles support the importance of the Sargasso Sea as a sea turtle nursery.

In-water behaviour and long-term movements of oceanic-stage juvenile sea turtles are not well described or quantified. This is owing to technological or logistical limitations of tracking small, fast-growing animals across long distances and time periods within marine habitats. Here, we present, to our knowledge, the first long-term offshore tracks of oceanic green turtles () in western North Atlantic waters.

No evidence of selection against anomalous scute arrangements between juvenile and adult sea turtles in Florida.

Variations in the number and arrangement of scutes often are used for species identification in hard-shelled sea turtles. Despite the conserved nature of scute arrangements, anomalous arrangements have been noted in the literature for over a century, with anomalies linked to sub-optimal environmental conditions in the nest during development. Long-held assumptions suggest that anomalous scute arrangements are indicative of underlying physiological or morphological anomalies, with presumed long-term survival costs to the individual.

Linking Ecology, Morphology, and Behavior to Conservation: Lessons Learned from Studies of Sea Turtles.

Here we describe examples of studies that have contributed both to a basic understanding of the biology of imperiled marine turtles, and to their management and conservation. Key elements include, first and foremost, correctly identifying species that differ strikingly in their morphology at different life stages because with growth, they change size by several orders of magnitude and have accompanying shape changes. We also review comprehensive field studies documenting the need for management actions to correct abnormal shifts in sex ratios caused by climate change.

Identifying Sex of Neonate Turtles with Temperature-dependent Sex Determination via Small Blood Samples.

Temperature-dependent sex determination, present in most turtle species, is a mechanism that uses temperature to direct the sex of the embryo. The rapid increase of global temperatures highlights the need for a clear assessment of how sex ratios of organisms with TSD are affected. In turtles with TSD, quantifying primary sex ratios is challenging because they lack external dimorphism and heteromorphic sex chromosomes.

Female loggerhead sea turtles ( L.) rarely remate during nesting season.

The goal of this study was to assess the consequences of single versus multiple paternity by identifying paternity of clutches per female to identify whether there were detectable costs or benefits. Multiple mating can occur when the benefits of mating outweigh the costs, but if costs and benefits are equal, no pattern is expected. Previous research on loggerhead sea turtle () populations found male-biased breeding sex ratios and multiple mating by many females nesting in southwestern Florida.

The impact of sand moisture on the temperature-sex ratio responses of developing loggerhead (Caretta caretta) sea turtles.

All species of sea turtles exhibit a cooler male/warmer female temperature-sex ratio response. Field and experimental studies on loggerhead sea turtle sex ratios suggest that increased sand moisture impacts sea turtle sex ratios with, and perhaps beyond, a cooling effect. This study examines how varying sand moisture impacts the embryo's response to temperature.

Pectoral and pelvic girdle rotations during walking and swimming in a semi-aquatic turtle: testing functional role and constraint.

Pectoral and pelvic girdle rotations play a substantial role in enhancing stride length across diverse tetrapod lineages. However, the pectoral and pelvic girdle attach the limbs to the body in different ways and may exhibit dissimilar functions, especially during locomotion in disparate environments. Here, we tested for functional differences between the forelimb and hindlimb of the freshwater turtle during walking and swimming using X-ray reconstruction of moving morphology (XROMM).

A new approach for measuring temperature inside turtle eggs.

For turtles, the thermal environment experienced during development plays critical roles in many biological processes. While the temperature inside an egg is assumed to match the substrate temperature, many factors such as evaporative cooling, metabolic heating and the insulating properties of extra-embryonic components can lead to thermal differences. However, no method developed to date has allowed for measurement of the embryonic temperature in live chelonian eggs.

Breeding sex ratio and population size of loggerhead turtles from Southwestern Florida.

Species that display temperature-dependent sex determination are at risk as a result of increasing global temperatures. For marine turtles, high incubation temperatures can skew sex ratios towards females. There are concerns that temperature increases may result in highly female-biased offspring sex ratios, which would drive a future sex ratio skew.

Hydric environmental effects on turtle development and sex ratio.

Experimental and field studies of different turtle species suggest that moisture influences embryonic development and sex ratios, wetter substrates tend to produce more males, and drier substrates produce more females. In this study, we used Trachemys scripta elegans to test the effect of moisture on embryonic development and sex ratios. T.

Environmental Causation of Turtle Scute Anomalies in ovo and in silico.

The turtle shell is often described as an evolutionary novelty that facilitated the radiation of the clade Testudines. The scutes, or keratinous plates, of the turtle shell are hypothesized to be patterned by reaction-diffusion dynamics, and this property of their development provides explanatory power to mechanisms of anomalous variation. A mathematical model of scute development predicts that anomalous variation in the phylogenetically stable pattern of scutes is achieved by environmental influence on the developmental program.

Experimental assessment of the effects of moisture on loggerhead sea turtle hatchling sex ratios.

Many reptiles have temperature-dependent sex determination (TSD). Sex determination in marine turtles is described by a cool-male, warm-female pattern. Nest sand temperature strongly influences sea turtle embryo development and sex differentiation.

An Immunohistochemical Approach to Identify the Sex of Young Marine Turtles.

Marine turtles exhibit temperature-dependent sex determination (TSD). During critical periods of embryonic development, the nest's thermal environment directs whether an embryo will develop as a male or female. At warmer sand temperatures, nests tend to produce female-biased sex ratios.

Seasonal Variation in Sea Turtle Density and Abundance in the Southeast Florida Current and Surrounding Waters.

Assessment and management of sea turtle populations is often limited by a lack of available data pertaining to at-sea distributions at appropriate spatial and temporal resolutions. Assessing the spatial and temporal distributions of marine turtles in an open system poses both observational and analytical challenges due to the turtles' highly migratory nature. Surface counts of marine turtles in waters along the southern part of Florida's east coast were made in and adjacent to the southeast portion of the Florida Current using standard aerial surveys during 2011 and 2012 to assess their seasonal presence.

The ontogeny of morphological defenses in Kemp's ridley (Lepidochelys kempii) and loggerhead (Caretta caretta) sea turtles.

Marine turtles are large reptiles that compensate for high juvenile mortality by producing hundreds of hatchlings during a long reproductive lifespan. Most hatchlings are taken by predators during their migration to, and while resident in, the open ocean. Their survival depends upon crypticity, minimizing movement to avoid detection, and foraging efficiently to grow to a size too difficult for predators to either handle or swallow.