FISH Still a Very Important Tool to Monitor Breast Cancer: Review of Recent Literature, Alternative Methods, and Proposed Techniques.

Fluorescent in situ hybridization has been the definitive modality in testing for overexpression of the Human Epidermal Growth Factor Receptor 2 (HER2) for decades to guide the appropriate treatment for cancer patients. In more recent years innovation and new techniques have been developed to supplant or even replace FISH as a standard method for biomarker testing. Alternative testing methods such polymerase chain reaction (PCR), next-generation sequencing (NGS), and other in situ hybridization (ISH)-derived techniques such as chromogenic-ISH (CISH) have been shown in multiple publications to have high concordance with FISH in addition to advantages in economics, logistics and practicality to the point where CISH and derived methods appear to have eclipsed FISH as a testing method of choice after immunohistochemistry (IHC).

Veterans to workplace: Keys to successful transition.

The purpose of the current research effort was two-fold: (a) determine what behaviors successful veteran job candidates perform during the job application process that distinguish them from less successful ones, and (b) identify the behaviors of successfully employed veterans that distinguish them from those that are less successful or unable to maintain civilian employment. To address these issues, a research initiative was undertaken by the City of Clarksville, TN; Montgomery County, TN; the Tennessee Department of Labor; and the Tennessee Department of Veterans Services. Researchers conducted a qualitative study in the environs of Nashville, TN and Clarksville, TN, where Fort Campbell a United States Army installation, is located.

Comparative study of ATR and DRIFT infrared spectroscopy techniques in the analysis of soil samples.

The primary goal of this investigation is to determine the feasibility of using vibrational spectroscopy techniques for the analysis of soil samples in order to characterize a specific area and hence create a soil map for use in forensic investigation. We compare spectra of "artifacts" which are exclusive to a single location or area rather than focus on the general intrinsic components of soil. Comparison of these artifacts may allow the creation of a map of different regions and thus allow matching of soil evidence to these regions (spectra).

Identification of client involvement in sex trafficking in Mississippi.

Objective: Sex trafficking is an unrelenting problem in Mississippi. No quantitative data currently exist on the prevalence of sex trafficking or the identification of victims in the state.

Method: This study used the Trafficking in Victims Identification Tool (TVIT) (Short Version) to identify the extent to which a sample of clients (n = 28) receiving services at a non-profit social services agency in Jackson, Mississippi, were also victims of sex trafficking.

MOTOR IMAGERY, PHYSICAL PRACTICE, AND MEMORY: THE EFFECTS ON PERFORMANCE AND WORKLOAD.

-The theoretical explanations used to explain changes in performance during motor imagery and physical practice conditions are inconsistent when memory retrieval is and is not required. This study measured performance time and workload during acquisition, a retention test requiring memory retrieval, and a retention test not requiring memory retrieval using a key-pressing task. The participants were assigned to physical practice with or without instructions to learn or motor imagery with or without instructions to learn.

Association among functional-movement ability, fatigue, sedentary time, and fitness in 40 years and older active duty military personnel.

Identifying potential modifiable determinants of functional movement ability and fatigue may inform efforts to maintain constant physical readiness, especially in active duty military over 40 years of age, who are largely sedentary throughout their work day. The primary aim of this study was to determine the associations among conventional fitness measures (body composition, flexibility, and strength), sedentary behavior (sitting time), functional movement ability, and fatigue in military personnel. Volunteer active duty personnel 40 years of age and older (n = 569 males; n = 121 females; mean ± SD for total sample = 44.

Expression of constitutively active CDK1 stabilizes APC-Cdh1 substrates and potentiates premature spindle assembly and checkpoint function in G1 cells.

Mitotic progression in eukaryotic cells depends upon the activation of cyclin-dependent kinase 1 (CDK1), followed by its inactivation through the anaphase-promoting complex (APC)/cyclosome-mediated degradation of M-phase cyclins. Previous work revealed that expression of a constitutively active CDK1 (CDK1AF) in HeLa cells permitted their division, but yielded G1 daughter cells that underwent premature S-phase and early mitotic events. While CDK1AF was found to impede the sustained activity of APC-Cdh1, it was unknown if this defect improperly stabilized mitotic substrates and contributed to the occurrence of these premature M phases.

A two process memory-based account for mental and physical practice differences.

The authors investigated the underlying processing structures for mental and physical practice. Participants mentally or physically performed 4 tasks during practice. Halfway through practice, 2 tasks were switched from mental to physical practice, or vice versa.

Effects of auditory feedback on movement time in a Fitts task.

The purpose of the present experiment was to investigate the role of auditory feedback and its impact on movement time in a standard Fitts task. Feedback was given at the moment of target acquisition. A 2-way analysis of variance found significant differences between feedback groups at all three indexes of difficulty (F(2, 40) = 156.

Behavior analysis in the era of medicalization: the state of the science and recommendations for practitioners.

Recent decades have witnessed an increase in biological explanations of common disorders such as depression and anxiety, a phenonmenon termed "medicalization" (Conrad, 2007). Behavior analysts may find it difficult to implement non-drug treatment with a populace that has become inundated with biological explanations and a preference for medication as the treatment of choice. Research frequently cited in support of medicalization includes studies of drug effectiveness, as well as family studies and studies of brain structure and function.

Influence of Angus and Belgian Blue bulls mated to Hereford x Brahman cows on growth, carcass traits, and longissimus steak shear force.

Steers and heifers were generated from Angus (A) and Belgian Blue (BB) sires mated to Brahman x Hereford (B x H) F(1) cows to characterize their growth, carcass traits, and LM shear force. A total of 120 B x H cows purchased from 2 herds and 35 bulls (14 A and 21 BB) produced calves during the 5-yr project. After the stocker phase, a representative sample of A- and BB-sired heifers and steers were transported to the Iberia Research Station to be fed a high-concentrate diet.

Effect of horn fly (Haematobia irritans) control on growth and reproduction of beef heifers.

The influence of horn fly control with commercially available ear tags was studied on beef replacement heifers (n = 670) for growth and reproductive performance. The study was conducted at five sites in Louisiana over 3 yr. Heifers used were yearling replacement females that were exposed to fertile bulls during a limited spring breeding season that coincided with the horn fly season.

Cow-calf and feedlot performances of Brahman-derivative breeds.

A study was conducted to compare Brangus, Beefmaster, Gelbray, and Simbrah breed influences for economically important traits. Brangus (9), Beefmaster (12), Gelbray (10), and Simbrah (7) sires were used in purebred and crossbred (Brahman x Hereford F1 cows) matings to generate calves (326) in eight breed groups. Beefmaster cows were of similar size (448 kg), Brangus and Gelbray cows were 11% heavier (501 and 503 kg), and Simbrah cows were 21% heavier (548 kg) compared to Brahman x Hereford F1 cows (452 kg).

Primary myelodysplastic syndrome with normal cytogenetics: utility of 'FISH panel testing' and M-FISH.

The myelodysplastic syndromes (MDS) are a clinically heterogeneous group of hematologic disorders. Cytogenetic analysis is crucial as it can provide both diagnostic and prognostic information. Herein, 32 cytogenetically normal patients with primary MDS were analyzed both by multiple FISH probes on interphase nuclei (FISH panel testing) and by M-FISH (metaphase nuclei).

Comparison of peripheral blood interphase cytogenetics with bone marrow karyotype analysis in myelofibrosis with myeloid metaplasia.

In a prospective study of 42 patients with myelofibrosis with myeloid metaplasia (MMM), peripheral blood (PB) and bone marrow (BM) interphase cytogenetics and PB CD34 enumeration were performed concomitantly with BM karyotype analysis. Interphase cytogenetics was performed with a panel of fluorescence in situ hybridization (FISH) probes that were capable of detecting most of the known recurrent cytogenetic lesions in MMM. There was a close concordance in the results of interphase cytogenetics between PB and BM, regardless of the PB CD34 count.

Diagnostic utility of fluorescence in situ hybridization in mantle-cell lymphoma.

Mantle-cell lymphoma (MCL) has a poorer prognosis than other small B-cell lymphomas, thus a definitive diagnosis is essential. The t(11;14)(q13;q32) associated with MCL juxtaposes portions of CCND1 (11q13) and IGH (14q32), resulting in over-expression of cyclin D1. In this study, a highly sensitive two-colour fluorescence in situ hybridization (FISH) method was developed to detect t(11;14)(q13;q32) in nuclei isolated from paraffin-embedded tissue.

Optimizing preparation of normal dendritic cells and bcr-abl+ mature dendritic cells derived from immunomagnetically purified CD14+ cells.

The goal of this work was to optimize dendritic cell (DC) preparations obtained from patients suffering from chronic myeloid leukemia (CML) and compare them with DC prepared from normal CD14+ mononuclear cells (MNC). We studied normal DC and bcr-abl+ leukemic DC (CML-DC) yields, expression of membrane molecules, differentiation status, and ability to stimulate T cells. We isolated DC precursors from PBMC by CD14-specific immunoadsorption and cultured them for 7 days in GM-CSF and IL-4, followed by a 3-day incubation to fully differentiate the cells.

Monosaccharides are not detected in whole or isthmic bovine oviductal fluid collected throughout the estrous cycle, as analyzed by HPLC.

In the bovine oviduct, monosaccharides may play a role in the preparation of gametes for fertilization. Sperm are sequestered in the isthmic region of the oviduct where capacitation, requisite biochemical changes in sperm membranes, may take place. Retention of spermatozoa in the oviductal isthmus is dependent on a carbohydrate recognition system between oviductal epithelium and sperm membrane lectins.

Differences in susceptibility to gastrointestinal nematode infection between Angus and Brangus cattle in south Louisiana.

Breed susceptibility to nematode infection was evaluated in Angus (Bos taurus) and Brangus (B. indicus crossbreed) cattle. A cow-calf herd and a yearling replacement heifer herd were monitored during one grazing season.

Atypical BCR and ABL D-FISH patterns in chronic myeloid leukemia and their possible role in therapy.

D-FISH uses DNA probes with fluorescence in situ hybridization to detect two fusion signals in cells with a t(9;22)(q34;q11.2) from patients with chronic myeloid leukemia (CML). Using D-FISH, 147 patients with CML were studied and considerable macro genetic variation was observed among their Ph-chromosomes.

Guidelines for buccal smear collection in breast-fed infants.

Buccal smear analysis is a noninvasive, fast, and relatively inexpensive diagnostic method. It is used commonly where rapid gender identification is necessary or, more recently, for detection of aneusomy, microdeletion syndromes, and a variety of polymerase chain reaction-based molecular genetic tests. Previously we have shown that maternal cells can contaminate buccal smears taken from breast-fed infants, resulting in difficulty with test interpretation.