Protocol for assaying irreversible inhibitors of thioredoxin reductase 1.

Selenoprotein thioredoxin reductase 1 (TXNRD1) is a promising therapeutic target, with several inhibitors reported to inhibit TXNRD1 activity. These inhibitors have the potential for applications such as anti-tumor medications. Here, we present a protocol for assessing irreversible inhibitors of TXNRD1.

Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif.

Thioredoxin reductase (TXNRD) is a selenoprotein that plays a crucial role in cellular antioxidant defense. Previously, a distinctive guiding bar motif was identified in TXNRD1, which influences the transfer of electrons. In this study, utilizing single amino acid substitution and Excitation-Emission Matrix (EEM) fluorescence spectrum analysis, we discovered that the guiding bar communicates with the FAD and modulates the electron flow of the enzyme.

Benzophenanthridine Alkaloid Chelerythrine Elicits Necroptosis of Gastric Cancer Cells via Selective Conjugation at the Redox Hyperreactive C-Terminal Sec Residue of Cytosolic Selenoprotein Thioredoxin Reductase.

Targeting thioredoxin reductase (TXNRD) with low-weight molecules is emerging as a high-efficacy anti-cancer strategy in chemotherapy. Sanguinarine has been reported to inhibit the activity of TXNRD1, indicating that benzophenanthridine alkaloid is a fascinating chemical entity in the field of TXNRD1 inhibitors. In this study, the inhibition of three benzophenanthridine alkaloids, including chelerythrine, sanguinarine, and nitidine, on recombinant TXNRD1 was investigated, and their anti-cancer mechanisms were revealed using three gastric cancer cell lines.

A review on the synthesis of metal oxide nanomaterials by microwave induced solution combustion.

With the increasing awareness of environmental protection and health, the preparation of metal oxide nanomaterials by environmentally friendly methods is favored by more and more researchers both at home and abroad. The preparation of metal oxide nanomaterials by a microwave-induced solution combustion synthesis method can significantly reduce the reaction time, energy consumption, and the use of toxic chemicals, which is an energy-saving, environmentally friendly method for nanomaterials synthesis. In addition, the microwave-induced solution combustion synthesis (MISCS) method has many advantages such as fast reaction speed, high selectivity, small product size, and homogeneous composition.

The molecular characteristics of avian influenza viruses (H9N2) derived from air samples in live poultry markets.

Objective: To study the molecular characteristics of H9N2-subtype avian influenza viruses (AIVs) isolated from air samples collected in live poultry markets (LPMs) and explore their sequence identities with AIVs that caused human infection.

Methods: Weekly surveillance of H9N2-subtype AIVs in the air of LPMs was conducted from 2015 to 2016. H9-positive samples were isolated from chicken embryos.

[Study of the bacterial community structure of microbiota in bacterial vaginosis using amplified ribosomal DNA restriction analysis].

Objective: To study the bacterial community structure of the microbiota in the vaginal fluid from patients with bacterial vaginosis.

Methods: The composition of bacteria in the samples of vaginal fluid from 3 patients with bacterial vaginosis and 1 normal premenopausal control was investigated by amplified ribosomal DNA restriction analysis(ARDRA).

Results: Lactobacillus species were the predominant bacteria in the woman without bacterial vaginosis.