Fluorescence and colorimetric rapid dual-signal "on-off-on" switching detection of ascorbic acid based on TSPP/DCIP.

A fluorescence and colorimetric dual-signal sensing system for the determination of ascorbic acid (AA) in complex media was developed by using 5,10,15, 20-tetrakis(4-sulfonyl) porphyrin (TSPP) as a sensing probe and sodium 2,6-dichloroindophenol (DCIP) as a bridge. A fluorescence resonance energy transfer (FRET) effect occurred when DCIP was added to the TSPP solution, resulting in the quenching of the fluorescence signal of TSPP and a change in the color of the solution from pink to blue. The DCIP in the system reacted with AA in a redox reaction to produce colorless phenol imine, the color of the solution changed from blue to green causing an obvious colorimetric response, and the TSPP/DCIP sensing system's fluorescence signal restored owing to AA introduced.

Preparation of copper nanoparticles fluorescent probes and detection of hydrogen peroxide and glucose.

Fluorescent copper nanoparticles (CuNPs) was synthesized by one-step chemical reduction method using ascorbic acid (AA) and copper sulfate (CuSO⋅5HO) as raw materials, which had good water solubility and fluorescence properties. A green, simple and safe CuNPs@Fe fluorescence probe was developed for the detection of hydrogen peroxide and glucose using Fe as a bridge. The prepared CuNPs could obtain the maximum fluorescence emission wavelength at 440 nm when the excitation wavelength was 360 nm.

One-Step Formation of Protein-Based Tubular Structures for Functional Devices and Tissues.

Tubular biological structures consisting of extracellular matrix (ECM) proteins and cells are basic functional units of all organs in animals and humans. ECM protein solutions at low concentrations (5-10 milligrams per milliliter) are abundantly used in 3D cell culture. However, their poor "printability" and minute-long gelation time have made the direct extrusion of tubular structures in bioprinting applications challenging.

[Expression of survivin protein in prostatic carcinoma tissues and its correlation with apoptosis of cancer cells].

Objective: To investigate the expression of survivin protein in the tissues of prostatic carcinoma and its correlation with apoptosis of cancer cells.

Methods: Expression of survivin protein and apoptosis index(AI) were detected by immunohistochemical and terminal deoxynucleotidyl transterase-mediated dUTP biotin nich end labeling(TUNEL) technique in the tissues of 42 cases of prostatic carcinima (PCa) and 10 cases of normal prostate (NP).

Results: Survivin prosteins were expressed in 34 of the 42 (80.

[A survey of the long-term incidence rate of benign prostate hyperplasia after vasectomy].

Objective: To study the relationship of the long-term incidence rate of benign prostate hyperplasia (BPH) after vasectomy to age and time after operation.

Method: Questionnaire survey, physical examination, and ultrasonography were conducted among 850 healthy males, aged 55 - 76 who underwent vasectomy more than 20 years before and 390 healthy males, aged 53 - 75, who had not undergone vasectomy in Yichang, Hubei province, selected by randomized cluster sampling. The data such as international prostate symptom scoring (IPSS), quality of life (QOL) index, maximum urine flow rate (MFR), and average flow rate (AFR), digital rectal examination (DRE), volume of prostate gland (V), and residual urine (R) were analyzed.

[Experimental study of vasoligation in the treatment and prevention of benign prostatic hyperplasia].

Objectives: To investigate the possibility and efficacy of vasoligation and deferent vein ligation in treating and preventing benign prostatic hyperplasia(BPH).

Methods: 40 male pooches were divided into A, B, C and D groups randomly. Each group has 10 pooches group A and B were made into the model of BPH.