Synergistic Effects of Caffeoylquinic Acid Compounds with Levofloxacin Against Uropathogenic : Insights from Molecular Dynamics Simulations, Antibiofilm, and Antimicrobial Assessments.

Urinary tract infections (UTIs), primarily caused by uropathogenic (UPEC), have high morbidity and recurrence rates. Resistance to levofloxacin hydrochloride (LEV), a commonly used treatment for UTIs, is increasingly problematic, exacerbated by biofilm formation mediated by interactions between cyclic di-GMP (c-di-GMP or CDG) and YcgR. In this study, we identified three caffeoylquinic acid compounds from -chlorogenic acid (CGA), sibiricose A5 (Si-A5), and 3--caffeoylquinic acid methyl ester (CAM)-that target YcgR through molecular docking.

Estimation of winter wheat LAI based on color indices and texture features of RGB images taken by UAV.

Background: Leaf area index (LAI) is an important indicator for assessing plant growth and development, and is also closely related to photosynthesis in plants. The realization of rapid accurate estimation of crop LAI plays an important role in guiding farmland production. In study, the UAV-RGB technology was used to estimate LAI based on 65 winter wheat varieties at different fertility periods, the wheat varieties including farm varieties, main cultivars, new lines, core germplasm and foreign varieties.

Synthesis, biological activities and mechanism studies of 1,3,4-oxadiazole analogues of petiolide A as anticancer agents.

In order to develop new natural product-based anticancer agents, a series of 1,3,4-oxadiazole analogues based on petiolide A were prepared and evaluated for their anticancer activities by MTT method. The structures of all analogues were characterized by various spectral analyses, and B9 was further confirmed by X-ray crystallography. Among all the synthesized compounds, B1 displayed the most promising growth inhibitory effect on colon cancer cells (HCT116) with the IC value of 8.

Two New Compounds Isolated from the Itea omeiensis and Their Anti-oxidant Activities.

Two new compounds (1-2) together with ten known compounds (3-12) were isolated for the first time from the 95 % EtOH extract of aerial parts of Itea omeiensis. Their structures were elucidated based on extensive spectroscopic analyses and comparison with published data. The structure of 1 was further confirmed through single-crystal X-ray diffraction analysis, and circular dichroism (CD) spectrum in combination with acid hydrolysis was employed for the absolute configuration determination of 2.

Exploring the hypoglycemic mechanism of chlorogenic acids from Pyrrosia petiolosa (Christ) Ching on type 2 diabetes mellitus based on network pharmacology and transcriptomics strategy.

Ethnopharmacological Relevance: Pyrrosia petiolosa (Christ) Ching (YBSW) is a Traditional Chinese medicine rich in chlorogenic acids. It is an important component in many Traditional Chinese medicinal hypoglycemic formulas and is commonly used by the Miao people to treat diabetes with good efficacy. Our previous research has suggested that chlorogenic acids may be the active ingredients in YBSW.

Liver protein and metabolite biolabels reveal hepatoprotective effects and active compounds of Eucommiae folium: Exploration of new application of herb based on multi-omics and bioinformatics.

A biolabel-led research based on multi-omics and bioinformatics was applied to analyze the application value of Eucommiae folium (EF) in liver cirrhosis, as well as the mechanism of action and material basis. Multi-omics were used to analyze the biolabels and key pathways of EF intervention in liver tissue. Subsequently, based on the information, bioinformatics was used to analyze the application value of EF in liver disease, as well the mechanism of action and material basis.

Sophora tonkinensis and active compounds inhibit mitochondrial impairments, inflammation, and LDLR deficiency in myocardial ischemia mice through regulating the vesicle-mediated transport pathway.

Ancient Chinese medicine literature and modern pharmacological studies show that Sophora tonkinensis Gagnep. (ST) has a protective effect on the heart. A biolabel research based on omics and bioinformatics and experimental validation were used to explore the application value of ST in the treatment of heart diseases.

Improvement of the YOLOv5 Model in the Optimization of the Brown Spot Disease Recognition Algorithm of Kidney Bean.

The kidney bean is an important cash crop whose growth and yield are severely affected by brown spot disease. Traditional target detection models cannot effectively screen out key features, resulting in model overfitting and weak generalization ability. In this study, a Bi-Directional Feature Pyramid Network (BiFPN) and Squeeze and Excitation (SE) module were added to a YOLOv5 model to improve the multi-scale feature fusion and key feature extraction abilities of the improved model.

Cardioprotection of Eucommiae Folium: the extract and pharmaceutically active compounds attenuate hyperglycemia, mitochondrial dysfunction, calcium dyshomeostasis, insulin resistance, and oxidative stress in diabetic cardiomyopathy db/db mice.

Objectives: A biolabel research based on multi-omics, informatics, molecular docking, and experimental verification was used to investigate the cardioprotective effect and pharmaceutically active compounds of Eucommiae Folium (EF).

Key Findings: Based on the biolabel research pattern, metabonomics, proteomics, and bioinformatics indicated that EF has a therapeutic potential for a variety of heart diseases, especially cardiomyopathy, and the most critical mechanism involved is the diabetic cardiomyopathy pathway. Bioinformatics, cheminformatics, and molecular docking showed that 24 EF compounds may play a therapeutic role in diabetic cardiomyopathy via this pathway.

A novel method for maize leaf disease classification using the RGB-D post-segmentation image data.

Maize ( L.) is one of the most important crops, influencing food production and even the whole industry. In recent years, global crop production has been facing great challenges from diseases.

Two new 2-arylbenzo[]furans from and their anti-hepatocellular carcinoma and anti-oxidant effects.

Two new 2-arylbenzo[]furans (-) and ten known compounds (-) were identified from the 95% EtOH extract of the branches and leaves of for the first time. Their structures were determined mainly based on extensive analyses of UV, IR, 1D/2D NMR and HRMS spectra. The results of MTT assays demonstrated the anti-tumor potential of compound with good selectivity, which displayed moderate inhibitory effects on proliferation of SK-hep-1 cells with IC value of 22.

Hydroxide-Mediated SAr Rearrangement for Synthesis of Novel Depside Derivatives Containing Diaryl Ether Skeleton as Antitumor Agents.

A simple and efficient hydroxide-mediated SAr rearrangement was reported to synthesize new depside derivatives containing the diaryl ether skeleton from the natural product barbatic acid. The prepared compounds were determined using H NMR, C NMR, HRMS, and X-ray crystallographic analysis and were also screened in vitro for cytotoxicity against three cancer cell lines and one normal cell line. The evaluation results showed that compound possessed the best antiproliferative activity against liver cancer HepG2 cell line and low toxicity, which made it worth further study.

Study on 2-arylbenzo[]furans from and their fragmentation patterns with Q-Orbitrap mass spectrometry.

One new 2-arylbenzo[]furan named iteafuranal F () as well as two known analogues () were isolated from the 95% EtOH extract of aerial parts of . Their chemical structures were constructed based on extensive analyses of UV, IR, 1D/2D NMR and HRMS spectra. Antioxidant assays revealed significant superoxide anion radical scavenging capacity of with IC value of 0.

Semisynthesis, Biological Evaluation and Molecular Docking Studies of Barbatic Acid Derivatives as Novel Diuretic Candidates.

Barbatic acid, a compound isolated from lichen, has demonstrated a variety of biological activities. In this study, a series of esters based on barbatic acid (-) were designed, synthesized, and evaluated for their diuretic and litholytic activity at a concentration of 100 μmol/L in vitro. All target compounds were characterized using H NMR, C NMR, and HRMS, and the spatial structure of compound was confirmed using X-ray crystallography.

Eucommiae Folium and Active Compounds Protect Against Mitochondrial Dysfunction-Calcium Overload in Epileptic Hippocampal Neurons Through the Hypertrophic Cardiomyopathy Pathway.

Epilepsy is a chronic brain disease and often occurs suddenly for no reason. Eucommiae folium (EF), an edible herb, can be used in the treatment of various kinds of brain diseases in clinic. From the perspective of safety and efficacy, EF is especially suitable for the treatment of chronic brain diseases.

Non-destructive monitoring of maize LAI by fusing UAV spectral and textural features.

Leaf area index (LAI) is an essential indicator for crop growth monitoring and yield prediction. Real-time, non-destructive, and accurate monitoring of crop LAI is of great significance for intelligent decision-making on crop fertilization, irrigation, as well as for predicting and warning grain productivity. This study aims to investigate the feasibility of using spectral and texture features from unmanned aerial vehicle (UAV) multispectral imagery combined with machine learning modeling methods to achieve maize LAI estimation.

Study on Hyperspectral Monitoring Model of Total Flavonoids and Total Phenols in Tartary Buckwheat Grains.

Tartary buckwheat is a common functional food. Its grains are rich in flavonoids and phenols. The rapid measurement of flavonoids and phenols in buckwheat grains is of great significance in promoting the development of the buckwheat industry.

Identification of structural variations related to drought tolerance in wheat (Triticum aestivum L.).

Structural variations are common in plant genomes, affecting meiotic recombination and distorted segregation in wheat. And presence/absence variations can significantly affect drought tolerance in wheat. Drought is a major abiotic stress limiting wheat production.

[Hyperspectral monitoring on proline content in winter wheat under water stress].

Frequent occurrence of drought disaster will seriously affect the growth and development of winter wheat (). We set different water stress treatments (80%, 60%, 45%, 35%, 30% of field water capacity) to simulate the severity of drought disaster. We measured free proline content (Pro) of winter wheat, and investigated the responses of Pro to canopy spectral reflectance under water stress.

Qualitative and quantitative method for quality control of Itea ilicifolia based on antioxidant Q-markers.

Itea ilicifolia Oliv is a folk medicine with antioxidant potential. In this study, the fingerprints of 14 batches of I. ilicifolia were established by HPLC with 17 common peaks.

Nitrogen fertilizer application rates and ratios promote the biochemical and physiological attributes of winter wheat.

Improper optimization of the rates and ratios of nitrogen application reduces grain yields and increases the nitrogen loss, thereby affecting environmental quality. In addition, scarcer evidence exists on the integrative approach of nitrogen, which could have effects on the biochemical and physiological characteristics of wheat. Treatments were arranged as nitrogen (N) rates of 00, 75, 150, 225, and 300 kg ha in the main plots, and different nitrogen ratios were organized in subplots at 5:5:0:0 and 6:4:0:0, which were applied at the sowing, jointing, flowering, and grain filling stages.

Natural products as sources of acetylcholinesterase inhibitors: Synthesis, biological activities, and molecular docking studies of osthole-based ester derivatives.

Osthole is a natural coumarin compound which isolated from Cusson, has extensive pharmacological activities and could be used as a leading compound for drug research and development. In a continuous effort to develop new acetylcholinesterase inhibitors from natural products, eighteen osthole esters were designed, synthesized, and confirmed by H NMR, C NMR and HRMS. The anti-AChE activity of These derivatives was measured at a concentration of 1.

Omics combined with network pharmacology reveal the neuroprotective mechanism of Sophora tonkinensis based on the biolabel research pattern: The treatment of Parkinson's disease against oxidative stress and neuroexcitatory toxicity.

Based on the biolabel research pattern, omics and network pharmacology were used for exploring the neuroprotection of Sophora tonkinensis (ST) in the treatment of brain diseases. Multi-omics were applied to investigate biolabels for ST intervention in brain tissue. Based on biolabels, the therapeutic potential, mechanism and material basis of ST for treating brain diseases were topologically analyzed by network pharmacology.

Terpenoids as anti-inflammatory substances inhibiting COX-2 isolated from the fibrous roots of (Lour.) Harms.

A new sesquiterpene, 1-carbonyl-2,8-dihydroxy-11-oxabicyclo [4,4,1] germacra- 2(3),4(5),6(7),8(9)-tetraene () and four known compounds (3, 23)-3-caffeoyl-23-coumaroylhederagenin (), (3, 23)-dicoumaroylhederagenin (), morettinone (), 24-ehylcholesta-3,6-dione () were isolated from the ethyl acetate layer of the fibrous root of (Lour.) Harms. The structure of compound was characterized by its H-NMR, C-NMR, DEPT, HMBC, HSQC spectrums, and the structures of the known compounds were determined by comparison of their spectroscopic data with those reported by the literatures.

Improving Winter Wheat Photosynthesis, Nitrogen Use Efficiency, and Yield by Optimizing Nitrogen Fertilization.

Wheat is the third most producing crop in China after maize and rice. In order to enhance the nitrogen use efficiency (NUE) and grain yield of winter wheat, a two-year field experiment was conducted to investigate the effect of different nitrogen ratios and doses at various development stages of winter wheat ( L.).