Relationship between serum vitamin C and serum uric acid in people with different BMIs: results from the NHANES 2017-2018 and Mendelian randomization study.

Objective: To examine the association of overweight/obesity and serum vitamin C (serum VC) with serum uric acid (SUA) and to assess causality using Mendelian randomization (MR).

Methods: 4,772 participants from the National Health and Nutrition Examination Survey (NHANES), 2017-2018 were included in this study. Multivariate linear regression, variance inflation factor and quantile regression were used to analyze the relationships between overweight/obesity and serum VC and SUA levels.

The validity of the subspecies, Lee, from complete mitochondrial genome.

The complete mitochondrial genome (mtgenome) was determined from the emerged-pupa shell (Noninvasive sampling) of Lee. It was 15,234 base pairs in length and contained 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. By taking and as outgroups, a maximum-likelihood phylogenetic tree was constructed among five geographical populations of based on 13 PCGs and two rRNA genes.

Intrinsically ESIPT-exhibiting and enhanced emission in polymer nanoparticles as signaling for sensing nitrite.

A straightforward approach to the fabrication of intrinsically excited-state intramolecular proton transfer (ESIPT)-fluorescent polymer nanoparticles (e-PNPs) was developed. The e-PNPs were obtained by self-assembly of the homopolymers derived from 4-aminosalicylic acid in aqueous solution. By incorporating ESIPT modules into polymer nanoparticles, the ESIPT reaction can be endowed with moderate hydrophobic micro-environment by nanoparticle scaffolds, eliciting enhanced ESIPT emission.

Physiological alterations of GS-CHO cells in response to adenosine monophosphate treatment.

Growth-arrested strategies (e.g. hypothermia and hyperosmolarity) have been widely employed to enhance cell-specific productivity (qP) in mammalian cell culture bioprocess.

Intrinsically fluorescent and highly functionalized polymer nanoparticles as probes for the detection of zinc and pyrophosphate ions in rabbit serum samples.

Intrinsically fluorescent polymer nanoparticles (F-PNPs) were synthetized from 2-hydroxy-5-methylisophthalaldehyde and melamine by solvothermal method. F-PNPs can emit strong yellow green fluorescence at 542 nm without the conjugation to any external fluorescent agent and surface modification. Owing to the abundant amino and hydroxyl groups on their surface, the F-PNPs possess multiple binding sites, good biocompatibility and excellent water-solubility.

Highly cysteine-selective fluorescent nanoprobes based on ultrabright and directly synthesized carbon quantum dots.

Strongly green fluorescent carbon dots (CQDs) have been directly synthesized from 2,4-diaminophenylhydrazine and 2-hydroxy-5-methylisophthalaldehyde through a facile solvothermal method. The novel CQDs exhibit high fluorescence quantum yield and excellent water solubility due to the abundant amino and hydroxy groups on their surface. The use of the as-prepared CQDs combined with Cu constructed a "turn-on" switch cysteine-responsive nanoprobe.

A specific nanoprobe for cysteine based on nitrogen-rich fluorescent quantum dots combined with Cu.

As a new member of the carbon quantum-dot family, fluorescent nitrogen-rich quantum dots (NRQDs) were prepared by a mixed solvothermal method using 2-azidoimidazole and aqueous ammonia as reactants. These NRQDs are rich in nitrogen up to 40.2%, which are endowed with high fluorescence quantum yield, good photostability, water-solubility and favourable biocompatibility.

The relationship of metabolic burden to productivity levels in CHO cell lines.

The growing demand for recombinant therapeutics has driven biotechnologists to develop new production strategies. One such strategy for increasing the expression of heterologous proteins has focused on enhancing cell-specific productivity through environmental perturbations. In this work, the effects of hypothermia, hyperosmolarity, high shear stress, and sodium butyrate treatment on growth and productivity were studied using three (low, medium, and high producing) CHO cell lines that differed in their specific productivities of monoclonal antibody.

Understanding central carbon metabolism of rapidly proliferating mammalian cells based on analysis of key enzymatic activities in GS-CHO cell lines.

The central carbon metabolism (glycolysis, the pentose phosphate pathway [PPP], and the tricarboxylic acid [TCA] cycle) plays an essential role in the supply of biosynthetic precursors and energy. How the central carbon metabolism changes with the varying growth rates in the in vitro cultivation of rapidly proliferating mammalian cells, such as cancer cells and continuous cell lines for recombinant protein production, remains elusive. Based on relationships between the growth rate and the activity of seven key enzymes from six cell clones, this work reports finding an important metabolic characteristic in rapidly proliferating glutamine synthetase-Chinese hamster ovary cells.

[Determination of volatile organic compounds in workplace by portable gas-chromatography].

Objective: To establish a portable gas chromatography (GC) method for the on-site rapid determination of epoxyethane, furan, dichloromethane, benzene, toluene in workplace.

Methods: By using dynamic dilutor, the volatile organic compounds (VOCs) standard gas was prepared and drawn to the capillary column of GC (PID) for separation and analysis. Based on the retention time and peak area, the VOCs was identified and quantified.