Divergent populations of HIV-infected naïve and memory CD4+ T-cell clones in children on antiretroviral therapy.

Background: Naïve cells comprise 90% of the CD4+ T-cell population in neonates and exhibit distinct age-specific capacities for proliferation and activation. We hypothesized that HIV-infected naïve CD4+ T-cell populations in children on long-term antiretroviral therapy (ART) would thus be distinct from infected memory cells.

Methods: Peripheral blood naïve and memory CD4+ T cells from 8 children with perinatal HIV on ART initiated at age 1.

A Dual-Responsive Fe₃O₄@ZIF-8 Nanoplatform Combining Magnetic Targeting and pH Sensitivity for Low Back Pain Therapy.

Low back pain (LBP) resulting from sciatic nerve compression presents major challenges in pain management, as traditional therapies provide only short-term relief and pose risks of systemic toxicity. In this study, an innovative FeO@ZIF-8-RVC (FZR) dual-responsive nanoplatform is introduced that integrates magnetic targeting with pH-sensitive, sustained drug release to overcome these limitations. The FZR nanoplatform encapsulates ropivacaine (RVC) within the ZIF-8-coated FeO core, enabling precise and prolonged analgesia at the injury site through magnetic guidance and acid-triggered release.

Jiawei Kongsheng Zhenzhong Pill (JKZP) Alleviates Chronic Cerebral Hypoperfusion-Induced Hippocampal Synaptic Damage via S100A10/tPA/BDNF Pathway.

Aims: To evaluate the effects of Jiawei Kongsheng Zhenzhong Pill (JKZP) on rats with ischemic mild cognitive impairment (MCI) and investigate the underlying mechanisms.

Methods: The components of JKZP were analyzed using Q-Orbitrap high-resolution mass spectrometry (HRMS). The MCI rat model was prepared through gradual bilateral common carotid artery occlusion (BCCAO).

Emodin-8-O-β-D-glucopyranoside alleviates cholestasis by maintaining intestinal homeostasis and regulating lipids and bile acids metabolism in mice.

Cholestatic liver disease(CLD) is caused by impaired bile flow due to obstruction of the biliary tract, and long-term exposure to bile acids in the liver triggers inflammation, eventually leading to liver toxicity and liver fibrosis. Emodin-8-O-β-D-glucopyranoside(EG) is anthraquinone compound that is widely found in traditional Chinese medicine. It possessed antioxidative and anti-inflammatory activities.

Aortic strain, flow pattern and wall shear stress in a patient-specific compliant aorta replica using Shake-the-Box.

High-fidelity in vitro flow simulator in combination with high-dimensional flow visualization techniques can offer precise and comprehensive evaluation of aortic hemodynamics. However, it is particularly challenging to create a fully transparent aorta replica that faithfully mimics the aortic curvature and stiffness. In this study, we successfully manufactured a patient-specific compliant aorta phantom with a dilated ascending aorta that can be used in vitro hemodynamic study.

The anti-cancer activity of Dioscin: an update and future perspective.

Natural drugs have the advantages of multi-pathway, multi-target, low toxicity, and high efficiency, which make them widely used and effective in anti-tumor therapy. Dioscin is a steroidal saponin compound that can be extracted from Dioscaceae plants. In recent years, it has been found that Dioscin has potent anti-tumor effects, can inhibit tumor cell proliferation, induce apoptosis and autophagy, inhibits tumor cell metastasis, reverses multidrug resistance, and increases sensitivity to anticancer drugs, and thus inhibit tumor progression.

Transforming the tradition of discrete milk yield correction factors: A continuous 1-step DeLorenzo-Wiggans method.

Over the past decades, various methods have been proposed to estimate daily milk yields from partial yields. Many of these methods divide milking interval time into varied classes, assuming that the yield correction factors are constant within classes but vary between classes. The DeLorenzo and Wiggans (D-W) method has been widely used in the United States, typically following a 2-step process.

Metabolic dependency mapping identifies Peroxiredoxin 1 as a driver of resistance to ATM inhibition.

Metabolic pathways fuel tumor progression and resistance to stress conditions including chemotherapeutic drugs, such as DNA damage response (DDR) inhibitors. Yet, significant gaps persist in how metabolic pathways confer resistance to DDR inhibition in cancer cells. Here, we employed a metabolism-focused CRISPR knockout screen and identified genetic vulnerabilities to DDR inhibitors.

Effects of MeCP2 on chronic seizures and cognitive function in mice with temporal lobe epilepsy.

Mutations in methyl CpG binding protein 2 (MeCP2) are linked to Rett syndrome, in which epilepsy is one of the most well-described disorders. However, little is known about the specific role of MeCP2 during epileptogenesis. Our previous study has demonstrated that MeCP2 has a unique control on the development of mossy fiber sprouting (MFS) in the epileptic hippocampus.

Establishment of a novel large-scale targeted metabolomics method based on NFSWI-DDA mode utilizing HRMS and TQ-MS.

Metabolites identification is the major bottleneck in untargeted LC-MS metabolomics, primarily due to the limited availability of MS information for most detected metabolites in data dependent acquisition (DDA) mode. To solve this problem, we have integrated the iterative, interval, and segmented window acquisition concepts to develop an innovative non-fixed segmented window interval data dependency acquisition (NFSWI-DDA) mode, which achieves comparable MS coverage to data independent acquisition (DIA) mode. This acquisition strategy harnesses the strengths of both DDA and DIA, which could provide extensive coverage and excellent reproducibility of MS spectra.

Advances in Research on Southern Corn Rust, a Devasting Fungal Disease.

Southern corn rust (SCR), caused by the obligate biotrophic fungus Underw., represents one of the most devastating threats to maize production, potentially resulting in yield losses exceeding 50%. Due to global climate change and cropping practices, epiphytotics of SCR have been increasingly reported, and are progressively spreading from tropical and subtropical maize growing areas to higher latitude areas.

Genome-Wide Identification of the Trihelix Transcription Factor Family and Functional Analysis of in Maize.

The trihelix transcription factor, which is a plant-specific family, play a critical role in plant growth and development and stress responses. Drought is the main limiting factor affecting yield of maize (). However, the identification and characterization of this gene family in maize and its biological functions in response to drought stress have not been reported.

Quantitative redox proteomics links thioredoxin to heavy ion resistance in Deinococcus radiodurans.

Heavy ion radiotherapy is an effective treatment for tumors, but its therapeutic efficacy is limited in cancer cells with radiation resistance. Deinococcus radiodurans, well known for its extremely resisting various stresses, was used to explore radioresistant mechanism. We used quantitative redox proteomics to track the dynamic changes in the global redox state after C irradiation.

Prediction Model of Diabetes Remission at 1-Year after Sleeve Gastrectomy and Comparison with other Models.

Background: Although numerous prediction models are available for diabetes remission following metabolic bariatric surgery, few are based on sleeve gastrectomy (SG). This study aimed to establish a predictive model for type 2 diabetes mellitus (T2DM) remission following SG and evaluate the efficacy of existing predictive models.

Methods: Patient data were gathered from a cohort study titled "Longitudinal Study of Bariatric Surgery in Western China.

Dynamic parallel traction theoretical model for the application and validation in femoral neck fractures - a finite element analysis.

Study Objective: This study aims to validate the application effects of a novel theoretical model of dynamic parallel traction in the treatment of femoral neck fractures through three-dimensional finite element analysis. By simulating the femoral neck fracture model, we explore the promotional effect of dynamic parallel traction on fracture healing.

Method: A digital 3D femur model was constructed using high-resolution computed tomography data of the lower limbs of a 70-year-old elderly subject.

Research on the timing for subsequent water flooding in Alkali-Surfactant-Polymer flooding in Daqing Oilfield based on automated machine learning.

Determining the optimal timing for subsequent water flooding in Alkali-Surfactant-Polymer (ASP) flooding is essential to maximizing both the technical and economic outcomes of oilfield blocks. This study identified eight critical parameters that influence the benefits of ASP flooding and established parameter ranges based on data from completed blocks and actual field measurements. The optimal timing for subsequent water flooding was determined by evaluating cumulative net profit variations throughout the ASP flooding lifecycle.

ACAT1/SOAT1 maintains adipogenic ability in preadipocytes by regulating cholesterol homeostasis.

Maintaining cholesterol homeostasis is critical for preserving adipocyte function during the progression of obesity. Despite this, the regulatory role of cholesterol esterification in governing adipocyte expandability has been understudied. Acyl-coenzyme A (CoA):cholesterol acyltransferase/Sterol O-acyltransferase 1 (ACAT1/SOAT1) is the dominant enzyme to synthesize cholesteryl ester in most tissues.

Integration WGCNA with LC-MS data for evaluating the processing status and transformation rules of Ligustri Lucidi Fructus: A novel strategy for evaluating the processing technology of traditional Chinese medicines.

Ligustri Lucidi Fructus (LLF) is a traditional Chinese medicine (TCM) to treat hepatopathy and osteopathy. Wine-processed LLF (WLLF) was much more widely used than raw LLF (RLLF) in clinical practice, however, there is no consensus on processing time. To investigate the processing status of WLLF and transformation rules during processing, a UHPLC-Q-Orbitrap-MS method combined with data-independent acquisition (DIA) mode was firstly established and 227 compounds were identified or tentatively identified.

Programmed BRD9 Degradation and Hedgehog Signaling Activation via Silk-Based Core-Shell Microneedles Promote Diabetic Wound Healing.

Wound healing impairment in diabetes mellitus is associated with an excessive inflammatory response and defective regeneration capability with suppressed Hedgehog (Hh) signaling. The bromodomain protein BRD9, a subunit of the non-canonical BAF chromatin-remodeling complex, is critical for macrophage inflammatory response. However, whether the epigenetic drug BRD9 degrader can attenuate the sustained inflammatory state of wounds in diabetes remains unclear.

An Integrated Framework for Drought Stress in Plants.

With global warming, drought stress is becoming increasingly severe, causing serious impacts on crop yield and quality. In order to survive under adverse conditions such as drought stress, plants have evolved a certain mechanism to cope. The tolerance to drought stress is mainly improved through the synergistic effect of regulatory pathways, such as transcription factors, phytohormone, stomatal movement, osmotic substances, sRNA, and antioxidant systems.

Molecular Subtypes Based on Mitochondrial Oxidative Stress-related Gene Signature and Tumor Microenvironment Infiltration Characterization of Colon Adenocarcinoma.

Background: As the most common subtype of colorectal cancer, colorectal adenocarcinoma (COAD) still needs better prognostic stratification methods and new intervention targets. The mitochondrial stress response, linked to mitochondrial homeostasis and cancer metabolism, warrants further investigation.

Methods: We identified mitochondrial oxidative stress-related genes (MOS) associated with COAD prognosis through the TCGA and GEO databases.